1,493 research outputs found
Effect of Kinesio Taping on lower back pain
No full textANOTATION Author: Lucie Klesalova Institution: Rehabilition clinic LF in Hradec Kralove Name of the bachelor's thesis: Effects of Kinesio Taping on lower back pain Supervisor: Mgr. Zuzana Hamarova Number of pages: 70 Nuber of annexes: 19 Year: 2015 Key words: kinesiotape, effects of Kinesio Taping, techniques of Kinesio Taping, lowerback pain This bachelor thesis is focused on the theoretical overview of the method of Kinesio Taping. It introduces the effects and ways of kinesiotape applications, it points to the possible indications and contraindications for this method. The practical part examines a set of 15 probands with a lower back pain. The aim was to evaluate whether a single application of kinesiotape could reduce pain in the lower back. The data was collected through questionnaires and kinesiological examination. The results were recorded for better clarity in graphs and tables
Crimp in socialization of people with cerebral palsy
No full textTITLE: Crimp in socialization of people with cerebral palsy AUTHOR: Lucie Falcníková DEPARTMENT: Department of Special Education SUPERVISOR: doc. PaedDr. Vanda Hájková, Ph.D. ABSTRACT: The work is aimed at integration of people with disabilities into society. The aimis to capture and describe one of the most important parts in everyone's lives -integration not only in the class group but also approximation to the socialization into a whole society. The thesis is dividend into two parts.There are theoretical and practical parts. In the theoretical part we can find definitiv of cerebral palsy, its causes, incidence and forms. We also deal with socialization in different areas, whitch most affect the lives of respondents. The last komponent of the theoretical part is the isme of communication of children with cerebral palsy. The practical part describes the methods and results of research which captures socialization of respondents, especially thein perception of socialization. There search methodis a structured interview with open questions. KEYWORDS: Cerebralpalsy, dysarthria, socializationofthechildwithdisabilities, disabiliti, barrier
Application of chromatographic methods for the separation of metabolites of cholesterol
No full textAuthor: Bc. Lucie Tetrevová Title: The application of chromatographic methods for separtion metabolites of cholesterol Language: czech The proposed diploma thesis deals with the development and validation of analytical method for free and esterified cholesterol separation from blood serum and adrenal tissue samples. This method is neccessary due to the different metabolism of free and esterified cholesterol in the body and it enables the research of these particular pathways using stable isotope tracers. The solid phase extraction method was chosen, optimised and validated with following results: precision values 2.14% for serum cholesterol ester, 6.98% for tissue cholesterol esters, 2.91% for serum free cholesterol, 7.48% for tissue free cholesterol, limit of detection 1 mol/l and linear range 1-25 mmol/l were found. The stability of the derivative was tested for 30 days at temperatures 4 C and -25 C. The derivative was found to be stable for the whole time period in both temperatures. Laboratory temperature was not tested due to a high volatility of the solvent used. The developed method was used in experimental studies evaluating the influence of dietary cholesterol and septic shock to cholesterol synthesis rate
Lyme borreliosis
No full textAuthor: Lucie Kohoutova Title: Lyme borreliosis (Diploma thesis) University: Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Field of study: Pharmacy Background: We investigated the presence of Lyme disease in the area of Havlickuv Brod based on laboratory diagnostics of specific or non- specific antibodies in the blood serum and in the liquor. The aim was to define typical laboratory signs of illness and recommend racional methods in diagnosis of Lyme disease. Methods: We used the data from Havlickuv Brod hospital laboratory of immunology. Patients were dividend into the groups according to the results of basic tests (if there were immunoglobulines type M and type G in serum or liquor), sex, age, the day of blood collection, code of diagnosis, patient's doctor and the type of running tests (ELISA, westernblot). These groups were compared. Patients with at least one positive result in basic tests and with any of additional test at the same time were divided in compliance with relations amongst these types of tests. We tried to determine appropriate interval between taking samples during monitoring the dynamics of the disease by contribution- based method. Results: More threatned by Lyme borreliosis are older women and people over the age of fifty- five. The highest number of new..
Relation between dramatic text and inscenation
No full textLucie Kryzová in her thesis addresses the issue of the relation between dramatic text and inscenation. The work is divided into two parts ? theoretical and practical. Firstly, used terminology is defined in the theoretical part. The author deals with crucial theories of Czech theater science (Jiří Veltruský and Otakar Zich?s work). Subsequently, she explores when a controversial relation between dramatic text and scenic piece is created, addresses the directors? apriorism and investigates the question of stylization of visualized theater. The second part has rather personal touch as it deals with author?s personal experience. Lucie tries to apply the findings from the theoretical part in her own work. She describes six semesters of her undergraduate studies of Directory-Dramaturgy
Étude de la régulation de la voie mTOR par l’interleukine 15 et 18 dans les cellules Natural Killer
No full textNatural Killer (NK) cells are cytotoxic innate lymphocytes important in the response against intracellular pathogens and tumor cells. Their activity depends on the integration of a large set of intracellular and environmental cues, including antigenic signals, cytokine stimulation and nutrient availability. This integration is achieved by signaling hubs, such as the metabolic checkpoint kinase mechanistic target of rapamycin (mTOR). mTOR is a conserved protein kinase that controls cellular growth and metabolism in eukaryotic cells and, therefore, is essential for NK cells development and function. The objective of this PhD was to decipher the pathway regulating mTOR in primary NK cells. We show that interleukin 15 (IL-15) and IL-18, two cytokines that are key for NK cells activation, activate the mTOR complex 1 (mTORC1) in a synergic manner. This activation is essential for NK cell proliferation and metabolic reprogramming in vitro and in vivo. Through genetic and pharmacological approaches, we show that IL-15 and IL-18 activate mTORC1 in a non-canonical manner, independently of the molecular inhibitor TSC. Instead, mTORC1 activation relies on the concomitant activation of the PI3K-AKT and ERK pathways by IL-15 and a non-canonical p38-MK2/3 pathway by IL-18 in primary NK cells. These findings shed a new light on the activation of NK cells by cytokines, a knowledge that could be exploited to boost NK cell potential in therapeutic settings.Les cellules Natural Killer (NK) sont des lymphocytes innés cytotoxiques importants dans la réponse aux pathogènes intracellulaires et aux cellules tumorales. Leur activité repose sur l’intégration de nombreux signaux intracellulaires et environnementaux, comme les signaux antigéniques, les cytokines ou encore la disponibilité en nutriment. Ces signaux sont perçus au niveau de centre intégrateur comme la protéine mechanistic target of rapamycin (mTOR). mTOR est une protéine kinase qui contrôle la croissance et le métabolisme cellulaire dans les cellules eucaryotes et qui par conséquent est essentiel au développement et aux fonctions des cellules NK. L’objectifs de ces travaux de thèse était d’étudier la régulation de la voie mTOR dans les cellules NK primaires. Ainsi, nous avons montré que l’interleukine 15 (IL-15) et l’IL-18, deux cytokines essentielles à l’activation des cellules NK, activent le mTOR complex 1 (mTORC1) de manière synergique. Cette activation est essentielle pour la prolifération et l’activation métabolique des cellules NK, à la fois in vitro et in vivo. Par des approches génétique et pharmacologique, nous avons montré que l’IL-15 et l’IL-18 activent mTORC1 de manière non-canonique, indépendamment de la protéine TSC. Ainsi, l’activation de mTORC1 repose sur l’engagement concomitant de la voie PI3K-AKT et ERK par l’IL-15 et la voie p38-MK2/3 par l’IL-18 dans les cellules NK primaire. Ces résultats permettent une meilleure compréhension de l’activation des cellules NK par les cytokines, une connaissance importante pour le développement d’immunothérapies utilisant les cellules NK
The Abstract Author
No full textThe Bachelor's thesis focuses on the concept of so called abstract author. It deals with various forms of abstract author's subject developed in literary theory and narratology: scriptor of R. Barthes, implied author of W. Booth, model author of U. Eco or abstract author of W. Schmid. Futher, the differences between the concepts and the polemics that accompany them (Chatman, Rimmon-Kenanová etc.) are reflected
Étude de la régulation de la voie mTOR par l’interleukine 15 et 18 dans les cellules Natural Killer
No full textLes cellules Natural Killer (NK) sont des lymphocytes innés cytotoxiques importants dans la réponse aux pathogènes intracellulaires et aux cellules tumorales. Leur activité repose sur l’intégration de nombreux signaux intracellulaires et environnementaux, comme les signaux antigéniques, les cytokines ou encore la disponibilité en nutriment. Ces signaux sont perçus au niveau de centre intégrateur comme la protéine mechanistic target of rapamycin (mTOR). mTOR est une protéine kinase qui contrôle la croissance et le métabolisme cellulaire dans les cellules eucaryotes et qui par conséquent est essentiel au développement et aux fonctions des cellules NK. L’objectifs de ces travaux de thèse était d’étudier la régulation de la voie mTOR dans les cellules NK primaires. Ainsi, nous avons montré que l’interleukine 15 (IL-15) et l’IL-18, deux cytokines essentielles à l’activation des cellules NK, activent le mTOR complex 1 (mTORC1) de manière synergique. Cette activation est essentielle pour la prolifération et l’activation métabolique des cellules NK, à la fois in vitro et in vivo. Par des approches génétique et pharmacologique, nous avons montré que l’IL-15 et l’IL-18 activent mTORC1 de manière non-canonique, indépendamment de la protéine TSC. Ainsi, l’activation de mTORC1 repose sur l’engagement concomitant de la voie PI3K-AKT et ERK par l’IL-15 et la voie p38-MK2/3 par l’IL-18 dans les cellules NK primaire. Ces résultats permettent une meilleure compréhension de l’activation des cellules NK par les cytokines, une connaissance importante pour le développement d’immunothérapies utilisant les cellules NK.Natural Killer (NK) cells are cytotoxic innate lymphocytes important in the response against intracellular pathogens and tumor cells. Their activity depends on the integration of a large set of intracellular and environmental cues, including antigenic signals, cytokine stimulation and nutrient availability. This integration is achieved by signaling hubs, such as the metabolic checkpoint kinase mechanistic target of rapamycin (mTOR). mTOR is a conserved protein kinase that controls cellular growth and metabolism in eukaryotic cells and, therefore, is essential for NK cells development and function. The objective of this PhD was to decipher the pathway regulating mTOR in primary NK cells. We show that interleukin 15 (IL-15) and IL-18, two cytokines that are key for NK cells activation, activate the mTOR complex 1 (mTORC1) in a synergic manner. This activation is essential for NK cell proliferation and metabolic reprogramming in vitro and in vivo. Through genetic and pharmacological approaches, we show that IL-15 and IL-18 activate mTORC1 in a non-canonical manner, independently of the molecular inhibitor TSC. Instead, mTORC1 activation relies on the concomitant activation of the PI3K-AKT and ERK pathways by IL-15 and a non-canonical p38-MK2/3 pathway by IL-18 in primary NK cells. These findings shed a new light on the activation of NK cells by cytokines, a knowledge that could be exploited to boost NK cell potential in therapeutic settings
Étude de la régulation de la voie mTOR par l’interleukine 15 et 18 dans les cellules Natural Killer
No full textNatural Killer (NK) cells are cytotoxic innate lymphocytes important in the response against intracellular pathogens and tumor cells. Their activity depends on the integration of a large set of intracellular and environmental cues, including antigenic signals, cytokine stimulation and nutrient availability. This integration is achieved by signaling hubs, such as the metabolic checkpoint kinase mechanistic target of rapamycin (mTOR). mTOR is a conserved protein kinase that controls cellular growth and metabolism in eukaryotic cells and, therefore, is essential for NK cells development and function. The objective of this PhD was to decipher the pathway regulating mTOR in primary NK cells. We show that interleukin 15 (IL-15) and IL-18, two cytokines that are key for NK cells activation, activate the mTOR complex 1 (mTORC1) in a synergic manner. This activation is essential for NK cell proliferation and metabolic reprogramming in vitro and in vivo. Through genetic and pharmacological approaches, we show that IL-15 and IL-18 activate mTORC1 in a non-canonical manner, independently of the molecular inhibitor TSC. Instead, mTORC1 activation relies on the concomitant activation of the PI3K-AKT and ERK pathways by IL-15 and a non-canonical p38-MK2/3 pathway by IL-18 in primary NK cells. These findings shed a new light on the activation of NK cells by cytokines, a knowledge that could be exploited to boost NK cell potential in therapeutic settings.Les cellules Natural Killer (NK) sont des lymphocytes innés cytotoxiques importants dans la réponse aux pathogènes intracellulaires et aux cellules tumorales. Leur activité repose sur l’intégration de nombreux signaux intracellulaires et environnementaux, comme les signaux antigéniques, les cytokines ou encore la disponibilité en nutriment. Ces signaux sont perçus au niveau de centre intégrateur comme la protéine mechanistic target of rapamycin (mTOR). mTOR est une protéine kinase qui contrôle la croissance et le métabolisme cellulaire dans les cellules eucaryotes et qui par conséquent est essentiel au développement et aux fonctions des cellules NK. L’objectifs de ces travaux de thèse était d’étudier la régulation de la voie mTOR dans les cellules NK primaires. Ainsi, nous avons montré que l’interleukine 15 (IL-15) et l’IL-18, deux cytokines essentielles à l’activation des cellules NK, activent le mTOR complex 1 (mTORC1) de manière synergique. Cette activation est essentielle pour la prolifération et l’activation métabolique des cellules NK, à la fois in vitro et in vivo. Par des approches génétique et pharmacologique, nous avons montré que l’IL-15 et l’IL-18 activent mTORC1 de manière non-canonique, indépendamment de la protéine TSC. Ainsi, l’activation de mTORC1 repose sur l’engagement concomitant de la voie PI3K-AKT et ERK par l’IL-15 et la voie p38-MK2/3 par l’IL-18 dans les cellules NK primaire. Ces résultats permettent une meilleure compréhension de l’activation des cellules NK par les cytokines, une connaissance importante pour le développement d’immunothérapies utilisant les cellules NK
Étude de la régulation de la voie mTOR par l’interleukine 15 et 18 dans les cellules Natural Killer
No full textNatural Killer (NK) cells are cytotoxic innate lymphocytes important in the response against intracellular pathogens and tumor cells. Their activity depends on the integration of a large set of intracellular and environmental cues, including antigenic signals, cytokine stimulation and nutrient availability. This integration is achieved by signaling hubs, such as the metabolic checkpoint kinase mechanistic target of rapamycin (mTOR). mTOR is a conserved protein kinase that controls cellular growth and metabolism in eukaryotic cells and, therefore, is essential for NK cells development and function. The objective of this PhD was to decipher the pathway regulating mTOR in primary NK cells. We show that interleukin 15 (IL-15) and IL-18, two cytokines that are key for NK cells activation, activate the mTOR complex 1 (mTORC1) in a synergic manner. This activation is essential for NK cell proliferation and metabolic reprogramming in vitro and in vivo. Through genetic and pharmacological approaches, we show that IL-15 and IL-18 activate mTORC1 in a non-canonical manner, independently of the molecular inhibitor TSC. Instead, mTORC1 activation relies on the concomitant activation of the PI3K-AKT and ERK pathways by IL-15 and a non-canonical p38-MK2/3 pathway by IL-18 in primary NK cells. These findings shed a new light on the activation of NK cells by cytokines, a knowledge that could be exploited to boost NK cell potential in therapeutic settings.Les cellules Natural Killer (NK) sont des lymphocytes innés cytotoxiques importants dans la réponse aux pathogènes intracellulaires et aux cellules tumorales. Leur activité repose sur l’intégration de nombreux signaux intracellulaires et environnementaux, comme les signaux antigéniques, les cytokines ou encore la disponibilité en nutriment. Ces signaux sont perçus au niveau de centre intégrateur comme la protéine mechanistic target of rapamycin (mTOR). mTOR est une protéine kinase qui contrôle la croissance et le métabolisme cellulaire dans les cellules eucaryotes et qui par conséquent est essentiel au développement et aux fonctions des cellules NK. L’objectifs de ces travaux de thèse était d’étudier la régulation de la voie mTOR dans les cellules NK primaires. Ainsi, nous avons montré que l’interleukine 15 (IL-15) et l’IL-18, deux cytokines essentielles à l’activation des cellules NK, activent le mTOR complex 1 (mTORC1) de manière synergique. Cette activation est essentielle pour la prolifération et l’activation métabolique des cellules NK, à la fois in vitro et in vivo. Par des approches génétique et pharmacologique, nous avons montré que l’IL-15 et l’IL-18 activent mTORC1 de manière non-canonique, indépendamment de la protéine TSC. Ainsi, l’activation de mTORC1 repose sur l’engagement concomitant de la voie PI3K-AKT et ERK par l’IL-15 et la voie p38-MK2/3 par l’IL-18 dans les cellules NK primaire. Ces résultats permettent une meilleure compréhension de l’activation des cellules NK par les cytokines, une connaissance importante pour le développement d’immunothérapies utilisant les cellules NK
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