1,720,955 research outputs found
The construction and analysis of low TCF11/Nrf1 expressing plasmids : regarding transactivational ability and intracellular localization
No full textTCF11 is a regulatory transcription factor belonging to the CNC-bZIP family. The specific biological function of this protein is still unknown. However, knockout studies in mice have revealed its importance during embryo development, and other studies have also displayed its involvement in the cell’s defense system against oxidants and carcinogens. The transactivating ability and intracellular localization of TCF11 and the isoform Nrf1 have been studied in cells using high expression plasmids. Due to the recent findings that over-expression of TCF11 in transfected cells caused an increase in cell mortality, the need for lower TCF11/Nrf1 expressing plasmids emerged. An additional reason for constructing the low expression plasmids was to study the localization and transactivating abilities of the proteins at levels closer to the endogenous situation. The transactivating ability was estimated by measuring the luciferase activity in COS-1 cells transiently co-transfected with a reporter plasmid and a high or low TCF11/Nrf1 expressing plasmid. The intracellular localization images were acquired by means of epifluorescence and confocal microscopy. The initial low expression constructs proved unsuitable due to the empty vector’s ability to cause indirect activation of the reporter plasmid. The second set of constructs were low expression plasmids that permitted verification of the nuclear detainment of Nrf1. However, further intracellular compartmentalization could not be detected for either Nrf1 or TCF11. In addition, TCF11 displayed a higher transactivating ability compared to Nrf1
Investigating celiac disease using recombinant soluble MHC class II molecules
No full textCeliac disease (CD) is a chronic intestinal inflammation showing a strong association to HLA-DQ2.5. This thesis focused on the use of soluble MHC class II tetramers to examine the underlying mechanism for this selective CD association and also to develop a potential diagnostic tool based on the detection of gluten specific T cells.
DQ2.5 is associated with atypically high amounts of Ii-chain derived CLIP peptides. This phenotype was assigned to an abnormally low interaction with the peptide editing molecule HLA-DM, and high kinetic stability of CLIP peptides to DQ2.5. HLA-DQ2.2 is a highly similar molecule, but does not show strong CD association. Interestingly, this molecule displayed a CLIP low phenotype due to a drastically reduced kinetic stability for CLIP. This was assigned to a polymorphic residue causing the gain/loss of a hydrogen bond involved in peptide binding.
In addition, the application of a short bread challenge allowed for the detection of gluten reactive T cells in peripheral blood of CD patients using flow cytometry and MHC tetramers. This opens for the use of MHC tetramers as a diagnostic tool for diseases where the antigen and MHC restriction elements are known
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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