196,462 research outputs found
Sulle tracce del Sale. CD-Rom multimediale
No full textNell'ambito dell’iniziativa "Sulle tracce del Sale" organizzata dal Museo "Gemma 1786" del Museo del Dipartimento di Scienze della Terra dell'Università di Modena e Reggio Emilia, è stato realizzato il CD-Rom multimediale "Sulle tracce del Sale" in collaborazione con l'ISA Venturi di Modena.Il CD-Rom, che descrive gli aspetti storici, sociali e culturali di questo bene prezioso come presentati all'interno del percorso espositivo realizzato presso il Museo "Gemma 1786", è stato distribuito come gadget ai visitatori la mostra ed è scaricabile dal sito web del Progetto TED della Provincia di Modena:http://ted.scuole.provincia.modena.it/progetti/sulle_tracce_del_sale/index.ht
Evaluation of a wavelet-based compression algorithm applied to the silicon drift detectors data of the ALICE experiment at CERN
No full textThis paper evaluates the performances of a wavelet-based compression algorithm applied to the data produced by the silicon drift detectors of the ALICE experiment at CERN. This compression algorithm is a general purpose lossy technique, in other words, its application could prove useful even on a wide range of other data reduction's problems. In particular the design targets relevant for our wavelet- based compression algorithm are the following ones: a high- compression coefficient, a reconstruction error as small as possible and a very limited execution time. Interestingly, the results obtained are quite close to the ones achieved by the algorithm implemented in the first prototype of the chip CARLOS, the chip that will be used in the silicon drift detectors readout chain
Avian metapneumoviruses expressing Infectious Bronchitis virus genes are stable and induce protection
No full textThe study investigates the ability of subtype A Avian metapneumovirus (AMPV) to accept foreign genes and be used as a vector for delivery of Infectious bronchitis virus (IBV) QX genes to chickens. Initially the GFP gene was added to AMPV at all gene junctions in conjunction with the development of cassetted full length DNA AMPV copies. After recombinant virus had been recovered by reverse genetics, GFP positions supporting gene expression while maintaining virus viability in vitro, were determined. Subsequently, either S1 or nucleocapsid (N) genes of IBV were positioned between AMPV M and F genes, while later a bivalent recombinant was prepared by inserting S1 and N at AMPV MF and GL junctions respectively. Immunofluorescent antibody staining showed that all recombinants expressed the inserted IBV genes in vitro and furthermore, all recombinant viruses were found to be highly stable during serial passage. Eyedrop inoculation of chickens with some AMPV-IBV recombinants at one-day-old induced protection against virulent IBV QX challenge 3 weeks later, as assessed by greater motility of tracheal cilia from chickens receiving the recombinants. Nonetheless evidence of AMPV/IBV seroconversion, or major recombinant tracheal replication, were largely absent
Unexpected pattern in expression of AMPV proteins: possible explanations and implications
No full textAvian metapneumovirus RT-nested-PCR: A novel false positive reducing inactivated control virus with potential applications to other RNA viruses and real time methods.
No full textUsing reverse genetics, an avian metapneumovirus (AMPV) was modified for use as a positive control for
validating all stages of a popular established RT-nested PCR, used in the detection of the two major AMPV
subtypes (A and B). Resultant amplicons were of increased size and clearly distinguishable from those
arising from unmodified virus, thus allowing false positive bands, due to control virus contamination of
test samples, to be identified readily. Absorption of the control virus onto filter paper and subsequent
microwave irradiation removed all infectivity while its function as an efficient RT-nested-PCR template
was unaffected. Identical amplicons were produced after storage for one year. The modified virus is likely
to have application as an internal standard as well as in real time methods. Additions to AMPV of RNA
from other RNA viruses, including hazardous examples such HIV and influenza, are likely to yield similar
safe RT-PCR controls
Utilizzo di Reverse Genetics per la messa a punto di un controllo positivo per la rilevazione e distinzione, mediante RT Nested PCR, di Metapneumovirus aviare sottotipo A e B
No full textQuesto lavoro descrive l’utilizzo di metodiche di Reverse Genetics per la produzione di un virus geneticamente modificato in grado di generare nella RT Nested PCR standard precedentemente descritta, amplificati di dimensioni maggiori rispetto a quelli generati da virus non modificati. Tale virus, impiegato come controllo positivo, rende possibile evidenziare immediatamente eventuali contaminazioni
Avian metapneumoviruses expressing Infectious Bronchitis virus genes are stable and induce protection
No full textThe study investigates the ability of subtype A Avian metapneumovirus (AMPV) to accept foreign genes
and be used as a vector for delivery of Infectious bronchitis virus (IBV) QX genes to chickens. Initially
the GFP gene was added to AMPV at all gene junctions in conjunction with the development of cassetted
full length DNA AMPV copies. After recombinant virus had been recovered by reverse genetics, GFP
positions supporting gene expression while maintaining virus viability in vitro, were determined. Subsequently,
either S1 or nucleocapsid (N) genes of IBV were positioned between AMPV M and F genes,
while later a bivalent recombinant was prepared by inserting S1 and N at AMPV MF and GL junctions
respectively. Immunofluorescent antibody staining showed that all recombinants expressed the inserted
IBV genes in vitro and furthermore, all recombinant viruses were found to be highly stable during serial
passage. Eyedrop inoculation of chickens with some AMPV-IBV recombinants at one-day-old induced
protection against virulent IBV QX challenge 3 weeks later, as assessed by greater motility of tracheal
cilia from chickens receiving the recombinants. Nonetheless evidence of AMPV/IBV seroconversion, or
major recombinant tracheal replication, were largely absent
sj-xlsx-4-vet-10.1177_03009858231217224 – Supplemental material for Highly pathogenic avian influenza virus H5N1 infection in skua and gulls in the United Kingdom, 2022
No full textSupplemental material, sj-xlsx-4-vet-10.1177_03009858231217224 for Highly pathogenic avian influenza virus H5N1 infection in skua and gulls in the United Kingdom, 2022 by Fabian Z. X. Lean, Marco Falchieri, Natalia Furman, Glen Tyler, Caroline Robinson, Paul Holmes, Scott M. Reid, Ashley C. Banyard, Ian H. Brown, Catherine Man and Alejandro Núñez in Veterinary Pathology</p
sj-xlsx-5-vet-10.1177_03009858231217224 – Supplemental material for Highly pathogenic avian influenza virus H5N1 infection in skua and gulls in the United Kingdom, 2022
No full textSupplemental material, sj-xlsx-5-vet-10.1177_03009858231217224 for Highly pathogenic avian influenza virus H5N1 infection in skua and gulls in the United Kingdom, 2022 by Fabian Z. X. Lean, Marco Falchieri, Natalia Furman, Glen Tyler, Caroline Robinson, Paul Holmes, Scott M. Reid, Ashley C. Banyard, Ian H. Brown, Catherine Man and Alejandro Núñez in Veterinary Pathology</p
Test of the end-ladder prototype board of the ALICE SDD experiment
No full textThe paper presents an end-ladder card prototype of
the data acquisition chain of the ALICE SDD experiment. The
prototype includes most of the electronics devices that will be
applied to ALICE SDD experiment. The card interfaces with
the front-end electronics and with the counting room detector
data link via the interface card named CARLOS_rx. The
end_ladder PCB has been fully tested by providing control
signals and input vectors via a pattern generator and by
collecting output data via the detector data link
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