87,089 research outputs found
Eratomyia risaralda Amorim & Rindal 2010
No full textrisaralda Amorim & Falaschi, 2010: 56, (M terminalia), (F terminalia). Type locality: Colombia, Risaralda SFF, Otún Quimbaya, El Molinillo, 2,220 m, HT M (IavH). Refs.: Amorim & Falaschi, 2012: 3 (cat.). Material examined. COLOMBIA: Risaralda: SFF [Santuario de Fauna y Flora], Otún, Quimbaya, El Molinillo, 04º 43 ’S 75 º 34 ’W, 2220 m, 1 male HT, 2 females PT, 17.ii–04.iii. 2003, G. López Leg., (IavH, MZSP).Published as part of Falaschi, Rafaela Lopes & Amorim, Dalton De Souza, 2016, FAMILY RANGOMARAMIDAE, pp. 46-49 in Zootaxa 4122 (1) on page 48, DOI: 10.11646/zootaxa.4122.1.8, http://zenodo.org/record/26249
A estátua de Atenas na Palmeira de Eurymedon em Delfos. Arte, história e recepção de uma famosa oferta votiva
No full textThis paper intends to investigate the fortune of the Eurymedon palm
tree and the gilded statue of Athena that surmounted it, dedicated by the Athenians
in the sanctuary of Delphi after the battle at the Eurymedon. It will focus in particular
on the descriptions of the statue in two imperial sources, Plutarch and Pausanias. The
meanings and the values of the statue through time will be considered, encompassing
historical, religious, philosophical and art historical aspects, as well as discussing
the state of preservation of the monument. Moreover, the comparison between the
literary sources will yield a better understanding of the history of their transmission
and a clearer assessment of the impact of this new reading on our knowledge of the
Athena statue
Variations of DNA polymerase-alfa and -beta during prolonged stimulation of human lymphocytes.
No full textStimulation of human lymphocytes with phytohemagglutinin is known to induce an increase in overall DNA polymerase activity (DNA nucleotidyltransferase; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7). Previous work [Pedrali Noy, G., Dalprà, L. Pedrini, A. M., Ciarrocchi, G., Giulotto, E., Nuzzo, F. & Falaschi, A. (1974) Nucleic Acids Res. 1, 1183] has shown that two subsequent waves of induction of DNA polymerase can be observed in this system; a first wave occurs in parallel with the increase in DNA replication rate; a second one occurs when the DNA synthesis rate is returned to minimal levels; the second peak is parallel to a maximum in DNA ligase and DNase levels. In the present work we have measured the levels of the DNA polymerases-alpha and -beta in phytohemagglutinin-stimulated lymphocytes during a 12-day period; both enzymes are present at detectable levels at time zero; in correspondence to the peak of DNA synthesis rate (between the fourth and fifth day) a peak of DNA polymerase-alpha is observed, increasing by a factor of approximately 20-fold over the zero time value; subsequently, the level of DNA polymerase-alpha decreases in parallel with DNA synthesis rate. The DNA polymerase-beta is also increased in correspondence to the peak in DNA synthesis rate, but reaches its maximum at later times, between the eighth and tenth day of incubation. The capacity of stimulated lymphocytes to perform repair synthesis following UV damage was measured in the same cells used for the enzyme activity determinations; this capacity also shows two maxima: a first one correlated with the peak in DNA replication rate, and a second one correlated with the peak of DNA polymerase-beta. These data suggest a certain tendency to the specialization of functions in human cell DNA polymerases; the alpha-enzyme seems mainly correlated with DNA replication, whereas the beta-enzyme seems more correlated with the ability of the cell to perform repair type synthesis
Variations of DNA polymerase-alpha and -beta during prolonged stimulation of human lymphocytes
No full textStimulation of human lymphocytes with phytohemagglutinin is known to induce an increase in overall DNA polymerase activity (DNA nucleotidyltransferase; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7). Previous work [Pedrali Noy, G., Dalpra, L. Pedrini, A. M., Ciarrocchi, G., Giulotto, E., Nuzzo, F. & Falaschi, A. (1974) Nucleic Acids Res. 1, 1183] has shown that two subsequent waves of induction of DNA polymerase can be observed in this system; a first wave occurs in parallel with the increase in DNA replication rate; a second one occurs when the DNA synthesis rate is returned to minimal levels; the second peak is parallel to a maximum in DNA ligase and DNase levels. In the present work we have measured the levels of the DNA polymerases-alpha and -beta in phytohemagglutinin-stimulated lymphocytes during a 12-day period; both enzymes are present at detectable levels at time zero; in correspondence to the peak of DNA synthesis rate (between the fourth and fifth day) a peak of DNA polymerase-alpha is observed, increasing by a factor of approximately 20-fold over the zero time value; subsequently, the level of DNA polymerase-alpha decreases in parallel with DNA synthesis rate. The DNA polymerase-beta is also increased in correspondence to the peak in DNA synthesis rate, but reaches its maximum at later times, between the eighth and tenth day of incubation. The capacity of stimulated lymphocytes to perform repair synthesis following UV damage was measured in the same cells used for the enzyme activity determinations; this capacity also shows two maxima: a first one correlated with the peak in DNA replication rate, and a second one correlated with the peak of DNA polymerase-beta. These data suggest a certain tendency to the specialization of functions in human cell DNA polymerases; the alpha-enzyme seems mainly correlated with DNA replication, whereas the beta-enzyme seems more correlated with the ability of the cell to perform repair type synthesis
Recovery of vanadium from a previously burned heavy oil fly ash
No full textThe recovery of vanadium from heavy oil fly ash having a high carbon content was performed using a four-step process consisting of a preliminary burning in order to reduce the carbonaceous fraction, followed by an acid leaching and an oxidative precipitation of vanadium pentoxide. The preliminary burning was conducted in the temperature range 650 to 1150 °C, below the initial deformation temperature (IDT) of the fly ash. The temperature of the preliminary burning step was revealed to be a significant parameter. Above 950 °C various phenomena (fusion, volatilisation of V, formation of V-Ni refractory compounds) occurred that adversely affected the recovery of vanadium. The burning temperature of 850 °C was found to be the best as a result of the trade-off between the overall vanadium recovery yield (83%) and the V2O5 weight percentage in the precipitate (84.8%)
Continuously Variable Transition Probability HMM for Speech Recognition
No full textA new duration intrinsic model for improved speech recognition by HMM techniques is presented. Assuming an exponentially decaying time dependency of the states loop probability, the duration density can be factorized and a path early pruning theorem demonstrated. As a consequence, computational complexity is greatly reduced with respect to explicit models, whereas recognition performances improve considerably
High-resolution mapping of the origin of DNA replication in the hamster dihydrofolate reductase gene domain by competitive PCR.
No full textBy the use of a highly sensitive mapping procedure allowing the identification of the start sites of DNA replication in single-copy genomic regions of untreated, exponentially growing cultured cells (M. Giacca, L. Zentilin, P. Norio, S. Diviacco, D. Dimitrova, G. Contreas, G. Biamonti, G. Perini, F. Weighardt, S. Riva, and A. Falaschi, Proc. Natl. Acad. Sci. USA 91:7119-7123, 1994), the pattern of DNA replication of the Chinese hamster dihydrofolate reductase (DHFR) gene domain was investigated. The method entails the purification of short stretches of nascent DNA issuing from DNA replication origin regions and quantification, within this sample, of the abundance of different adjacent segments by competitive PCR. Distribution of marker abundance peaks around the site from which newly synthesized DNA had emanated. The results obtained by analysis of the genomic region downstream of the DHFR single-copy gene in asynchronous cultures of hamster CHO K1 cells are consistent with the presence of a single start site for DNA replication, located approximately 17 kb downstream of the gene. This site is coincident with the one detected by other studies using different techniques in CHO cell lines containing an amplified DHFR gene domain
La cistatina C sierica come marker di funzione renale nella valutazione del rischio cardiovascolare.
No full textFusarium oxysporum f. sp. dianthi transformed with marker genes as a tool for studying resistance in Dianthus
No full textFusarium oxysporum Schlechtend:Fr. f. sp. dianthi (Prill. & Delacr.) W. C. Snyd. & H. N. Hans. (Fod), the causal agent of a vascular disease known as Fusarium wilt, represents the most important pathogen of carnation (Dianthus caryophyllus L.), causing severe yield losses in susceptible cultivars. The pathogen is present in the soil profile in which carnation roots are distributed. Various biological races of Fod are currently known on the basis of their selective virulence against different carnation cultivars. In Italy, as in the rest of Europe, race 2 of the pathogen is the most prevalent1. The most important factor influencing wilt development is the cultivar resistance; different carnation cultivars are known to show from low to high levels of resistance against the different races of Fod.
Reporter proteins as Green Fluorescent Protein (GFP) and the Red Fluorescent Protein (DsRedFP) have been previously used to follow interactions of filamentous fungi with other organisms2 3. In this work, a highly pathogenic F. oxysporum f. sp. dianthi (Fod) strain has been transformed with genes coding for GFP and DsRedFP, as a tool to evaluate the colonization process and localize the pathogen in the root structure of carnation plants. Expression of both proteins resulted in bright green or red cytoplasmic fluorescence of mycelium when observed under fluorescent light. Stable transformants, selected on the basis of brightness and stability of fluorescence, were inoculated on roots of a partially resistant cultivar of carnation. Root and vessel colonization was evaluated, at regular times, by fluorescent microscopic observation of plant tissue sections. Autofluorescence of the carnation root was very high at the emission wavelength of the DsRed-Express so it was not possible to distinguish infecting hyphae within plant tissues. GFP transformed Fod was easily visualized on and within root tissues. The fungus invades the root apex without any preferential entry point and its growth seems to proceed randomly in the sub-apical zone. The hyphae are confined within the vascular cylinder by the endodermis cells beginning from the zone of differentiation of vascular tissues and are able to grow inside vessels.
The availability of a marked F. oxysporum f. sp. dianthi strain allows the monitoring of carnation root colonisation and may be a powerful tool for studying resistance processes easily and accurately. Furthermore the possibility to follow plant-microbe interactions can help in defining biological control measures by beneficial antagonistic microorganisms
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