111,893 research outputs found
CATALITIC ACTIVITY OF IMMOBILIZED FUMARASE
No full textIn order to evaluate the influence of the immobilization techniques of fumarase on its immobilization yield and on its
catalytic activity, it was covalently bonded to the surface of polymers (consisting of suitably functionalized ethylene–vinyl
alcohol copolymers and functionalized poly(acrylamides), and physically entrapped into cross-linked poly(acrylamide) gels.
The kinetic parameters of the hydration reaction of fumarate to l-malate were obtained by determining the UV absorbance
variation of the fumarate double bond at 290 nm.
When the enzyme is covalently bonded, both activity and stability of the enzymatic preparations are low; however, when fumarase is bonded to ethylene–vinyl alcohol copolymers by less denaturating and more spacing coupling agents (as glutaraldehyde), a better residual enzyme activity was obtained, and it was seen that this latter depended on the amount of bonded enzyme. Also the influence of the hydrophilicity of the polymer matrix on the amount of bonded enzyme and on its activity was evaluated. Satisfactory results were obtained by physical entrapment of the enzyme into poly(acrylamide) gel, with quantitative immobilization yields, a rather good enzyme activity (η = 38 ± 5%), and a constant catalytic activity, under operative conditions, for several days.
The inhibiting effect of methanol concentrations up to 20% (v/v), in the reaction medium, was also evaluated for the
different immobilized enzyme preparation
Combining two model systems of psychosis: The effects of schizotypy and sleep deprivation on oculomotor control and psychotomimetic states
No full textThis is the peer reviewed version of the following article: Meyhöfer, I, Steffens, M, Faiola, E, Kasparbauer, A‐M, Kumari, V, Ettinger, U. Combining two model systems of psychosis: The effects of schizotypy and sleep deprivation on oculomotor control and psychotomimetic states. Psychophysiology. 2017; 54: 1755– 1769., which has been published in final form at https://doi.org/10.1111/psyp.12917. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived VersionsHumboldt Research Awar
author-bios-SRD-19-0063.R1 – Supplemental material for The Network Structure of Police Misconduct
No full textSupplemental material, author-bios-SRD-19-0063.R1 for The Network Structure of Police Misconduct by George Wood, Daria Roithmayr and Andrew V. Papachristos in Socius</p
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Intensity And Energy Expenditure Of Sports In Elite Paralympic Athletes With Locomotor Disabilities
No full textSymposium Lessons Learned on the Road to Vancouver (Olympic and Paralympic Friday 3:15PM-5:15PM Andrew Pipe,FACSM,University of Ottawa
Sports Medicine Issues Committee) Heart Institute
Randall L.Wilber,FACSM,United States Olympic
Committee
Jos de Koning,FACSM,University of Amsterdam
Donald McKenzie,University of British Columbia
Mark Aubry,Ottawa Sport Medicine Centre
Marco Bernardi,University of Rom
Atrial fibrillation following chemotherapy for stage IIIE diffuse large B-cell gastric lymphoma in a patient with myotonic dystrophy (Steinert's disease)
No full textOxaliplatin/rituximab combination in the treatment of intermediate-low grade non-Hodgkin's lymphoma of elderly patients
No full textHigh and intermediate grade non-Hodgkin's lymphomas (NHL) require treatments with aggressive chemotherapy schedules. However, low grade NHLs display a low chemoresponsiveness and patients aged >65 years often do not tolerate anthracycline and corticosteroid-containing chemotherapy regimens. Therapeutic options in this subset of patients are watchful waiting, oral alkylating agents, purine nucleoside analogues, combination chemotherapy, interferon and monoclonal antibodies. The approval of rituximab, an unconjugated chimeric antibody against the CD20 antigen for the treatment of B-cell NHL marked a milestone in the development of antibody treatment. Moreover, promising results have also been found with oxaliplatin in patients with NHL and reversible, cumulative, peripheral sensory neuropathy is the principle dose-limiting factor of oxaliplatin therapy. On the basis of these considerations we have performed a feasibility study in NHL in patients aged >65 years using as schedule: 130 mg/m2 oxaliplatin every 21 days and 375 mg/m2 rituximab weekly. We have enrolled 8 patients, 2 males and 6 females (mean age 69.2+/-3.1 years; median, 67 years) affected by intermediate or high grade stage III/IV NHL. Six patients have cardiac abnormalities (myocardial function between 45 and 50%) and 1 increase of transaminasemia due to active chronic hepatitis. All the patients included in the study were treated for at least 3 cycles and 31 cycles were completed. We have recorded grade I/II (CTC) neurotoxicity in 30%, grade I anemia in 25% and grade I neutropenia in 20% of the patients. No infusional reactions, liver or renal toxicity neither nausea and/or vomiting were recorded. One complete response, 3 partial response and 3 minimal response were obtained at 11 months of median time follow-up. These results demonstrate the feasibility of this schedule which offers a suitable alternative regimen to treat elderly patients with NHL and shows a good efficacy and an acceptable toxicity profile
Once-per-cycle pegfilgrastim in breast cancer patients treated with docetaxel/epidoxorubicin/cyclophosphamide
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