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İzole Edilmiş Sıçan Pankreas Adacık Hücrelerine in Vitro Trombospondinin Etkisi
No full textPancreas islet cells are very sensitive to hypoxic stress because
of having small amount of anti-oxidant enzyme. Loss of islet cell
viability due to hipoxic damage decreases success of transplantation.
Islet- endothelial co-culture prevents the cell death and improves the
cell function. Furthermore, transplanted islets to portal system are
also in close contact with the endothelial cells. Some of the
endothelium derived substance improve the viability of islet cells and
some of them cause negative effects. Thrombospondin(TSP) is a
multifunctional substance that is released from islet endothelial cells
and its effect on islet cell is not clear.
The aim of this study is to investigate whether TSP, which is an
endothelial factor, is effective on the islet cell’s viability and function.
Isolated islet cells were divided into three groups: in the control
group no substance were added to the normal culture medium. In ER
stress generated group, 20 μg/ml tunicamycin (TUN) and in the third
group 200pM thrombospondin (TSP) was added. Then groups were
incubated for 24 hours. We measured islet cell viability with FDA/PI
and cell function with glucose stimulation. We explored ER stress with
western blot and oxidative stress parameters. We concluded that 200 pM TSP does not affect the cell viability
but cause cell dysfunction. It did not appeare to come by endoplasmic
reticulum stress or oxidative stress.
According to the results, TSP may cause damage in different
mechanisms like exocytosis in the islet cells. The results of this study
may be supported with in vivo experiments, various doses of TSP and
incubation periods. Additional studies are needed to clarify the
cellular mechanisms that cause functional loss.Pankreas adacık hücreleri az miktarda antioksidan enzime sahip
oldukları için hipoksik hasara çok duyarlıdırlar. Kültür ortamına konan
hücrelerin hipoksik hasar nedeniyle canlılıklarını kaybetmesi
transplantasyon başarısını düşürmektedir. Bu sorunu ortadan
kaldırmak için yapılan endotel hücrelerinin adacık hücre kültürüne
eklenmesi adacık hücre canlılık ve fonksiyonunda iyileşmeye neden
olmuştur. Ayrıca portal sisteme transplante edilen adacıklar endotel
hücreler ile yakın temas halindedirler. Endotelden salınan maddelerin
bir kısmı adacık canlılığını artırırken bir kısmı da olumsuz etki
etmektedir. Trombospondin(TSP) de adacık endotelinden salınan çok
fonksiyonlu bir madde olup adacık hücresi üzerindeki etkisi net olarak
bilinmemektedir.
Bu çalışmanın amacı TSP adacık hücre fonksiyon ve canlılığını
etkilemesi muhtemel bir endotelyal faktör olup olmadığını
araştırmaktır.
İzole edilen adacık hücreleri üç gruba ayrıldı. Kontrol grubuna
normal kültür ortamı dışında ek bir madde koyulmadı. Endoplasmik
retikulum (ER) stresi oluşturulacak grubun kültür ortamlarına 20
μg/ml tunikamisin (TUN) ve son grubun kültür ortamlarına 200pM TSP eklenerek 24 saat inkübe edildi. Floresein diasetat/ Propidyum
iyot (FDA/PI) ile canlılık, glukoz stimülasyonu ile fonksiyon
değerlendirildi. Oksidatif stres parametrelerine ve western blot ile ER
stresine bakıldı.
200 pM TSP’nin canlılığı etkilemediği fakat fonksiyon
bozukluğuna neden olduğu sonucuna ulaşıldı. Fonksiyon
bozukluğunun oksidatif hasar veya ER stresi nedeniyle meydana
gelmediği ortaya çıktı.
Bu sonuçlar, TSP’nin adacık hücrelerinde ekzositoz gibi bir
mekanizmayı bozmuş olabileceğini düşündürmektedir. In vivo
çalışmalar, farklı doz ve inkübasyon süreleri ile çalışmanın sonuçları
desteklenebilir. Fonksiyon kaybına neden olan hücresel mekanizmanın aydınlatılması için ek araştırmalara ihtiyaç vardır
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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