1,720,981 research outputs found
Ecological observations of pelagic bacterial and archaeal communities in the Atlantic-Arctic boundary zone
The global climate change has an unprecedented impact on the Arctic Ocean, resulting in warming of the Arctic surface air at much faster rates than the global average. The warming temperatures lead to constantly declining Arctic sea ice cover, which reached in September 2018 the sixth lowest summertime minimum extent in the satellite record (since the late 1970s). Shrinking sea ice has a strong impact on the entire Arctic marine ecosystem, through alterations of the primary production, grazers communities, and subsequently the biological carbon pump. Current predictions of entirely sea-ice free summers in the Arctic Ocean already in the second half of this century urges the need to understand the ongoing oceanographic and biological processes in order to predict how the Arctic ecosystem will respond to further environmental changes. The differentiation between natural temporal ecosystem variability and anthropogenically-induced impact of the climate change requires long-term observations. The Ocean Observing System FRAM (FRontiers in Arctic marine Monitoring), which was established in 2014, is an Arctic long-term observatory for investigating the impact of changing ocean properties and sea ice conditions of the Arctic Ocean on its marine ecosystem. The starting point for the FRAM project was the already existing long-term observatory HAUSGARTEN, situated in the main gateway between the Arctic and the Atlantic Oceans - the Fram Strait. To date, despite their importance for the biogeochemical cycling, very little is known regarding the diversity and function of microbial communities in the Arctic Ocean in general, and specifically in the Fram Strait. In the framework of FRAM, a Molecular Observatory was established, for conducting standardized molecular-based high-resolution observations of the Arctic microbial communities. This thesis was conducted as part of the FRAM Molecular Observatory, and as part of the establishment process of the observatory it contributes to the methodological and procedural standardization required for long-term microbial observations. This thesis provides a first comprehensive overview of currently existing long-term microbial observatories around the world, it provides guidelines for initial steps towards establishing a community network between them, and stresses the urgent need in community efforts towards methods standardization. Furthermore, as part of the methods standardization for long-term microbial observations, this thesis includes a performance comparison between two, broadly used in microbial oceanography, 16S rRNA gene primer sets. The main focus of the thesis is on the ecology of pelagic bacterial and archaeal communities in the Fram Strait. Its overall objective was to investigate the distribution of these communities in the Fram Strait, and to identify environmental drivers of their diversity. The observations of this thesis reveal that sea ice has a strong impact on the development of the seasonal phytoplankton bloom during the summer. As a result, sea ice conditions are affecting the bacterial diversity in surface water, and are leading to a distinct community in sea-ice free and sea-ice covered regions of the Fram Strait. However, the impact of the sea ice is not limited to the surface ocean, as it also heavily affects the vertical export of aggregated organic matter to the deep ocean. The results of this thesis also show that aggregates formed under the sea ice sink faster, and by that provide a stronger vector for transport of bacterial and archaeal taxa to the deep ocean, compared to ice-free waters. Altogether, this thesis contributes to the baseline knowledge needed for further long-term observations of pelagic microbial communities in the Arctic marine ecosystem. Furthermore, it provides an important insight into the strong impact of the sea ice on bacterial and archaeal communities throughout the entire water column, underlining the potential impact of further environmental changes on the Arctic Ocean in the light of prevalent global warming and climate change
Going Beyond Counting First Authors in Author Co-citation Analysis
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Why close a bacterial genome? The plasmid of Alteromonas macleodii HOT1A3 is a vector for inter-specific transfer of a flexible genomic island
Genome sequencing is rapidly becoming a staple technique in environmental and clinical microbiology, yet computational challenges still remain, leading to many draft genomes which are typically fragmented into many contigs. We sequenced and completely assembled the genome of a marine heterotrophic bacterium, Alteromonas macleodii HOT1A3, and compared its full genome to several draft genomes obtained using different reference-based and de-novo methods. In general, the de-novo assemblies clearly outperformed the reference-based or hybrid ones, covering>99% of the genes and representing essentially all of the gene functions. However, only the fully closed genome (~4.5Mbp) allowed us to identify the presence of a large, 148 kbp plasmid, pAM1A3. While HOT1A3 belongs to Alteromonas macleodii, typically found in surface waters (surface ecotype), this plasmid consists of an almost complete flexible genomic island, containing many genes involved in metal resistance previously identified in the genomes of Alteromonas mediterranea (deep ecotype). Indeed, similar to A. mediterranea, A. macleodii HOT1A3 grows at concentrations of zinc, mercury and copper that are inhibitory for other A. macleodii strains. The presence of a plasmid encoding almost an entire flexible genomic island suggests that wholesale genomic exchange between heterotrophic marine bacteria belonging to related but ecologically different populations is not uncommon
koamabayili/VECTRON-author-checklist: VECTRON author checklist
We have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
Author-wise bibliometric analysis based on entropy.</p
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