1,720,955 research outputs found
Zymographic Analysis of Matrix Metalloproteinases (MMP-2 and MMP-9) in Cerebrospinal Fluid and Sera from Patients with Multiple Sclerosis
No full textPOLYPHENOLS AS A POTENTIAL STRATEGY IN OVERCOMING THERAPY RESISTANCE IN ANAPLASTIC THYROID CANCER THROUGH THE SOX2-SOX17 MOLECULAR SWITCH
No full textAnaplastic thyroid cancer (ATC) is a rare and undifferentiated endocrine tumor, without reliable therapies and poor prognosis. Due to severe early metastasis and the rapid fatal course, surgery is rarely performed, while radio and chemotherapy exhibit low efficacy. Therapy resistance is largely attributed to a loss of thyroid cell function due to the
downregulation of thyroid terminal differentiation genes, among them sodium-iodide symporter (NIS), transcription termination factor (TTF-1), and thyroid peroxidase (TPO).
Disrupting the circuit that sustains the acquisition of a dedifferentiated state in ATC, remains the main challenge in order to resensitize cells to conventional or novel therapies. We
previously identified a cancer stem cell (CSC) subpopulation derived from ATC, characterized by high expression of several stem cell markers, such as SOX2, OCT4, and NANOG. We proposed a potential upstream role of SOX2 in regulating cell proliferation and tumor progression. In contrast, SOX17 displayed anti-tumoral properties in papillary thy-
roid cancer (PTC). Its low expression positively correlates with increased PTC cell migration and invasion. However, the impact of the SOX family on thyroid cancer is unclear.
Our previous in vitro studies shown that resveratrol (RSV), a natural polyphenol, could affect the stem cell features, promoting the differentiation towards the epithelial lineage by modulation of the SOX2/SOX17 balance in limbal primary mesenchymal stem cells. In this regard, two ATC cell lines, SW1736 and 8505c, were used to investigate the effects of
RSV and two natural analogues, 3,4’,5-trans-trimethoxystil-bene (3-MET-OX) and isorhapontigenin (ISOR-H-PG). Polyphenol treatments did not induce apoptosis, whereas a
slowdown in the cell cycle in anaplastic thyroid cells compared with normal thyroid cells (Nthy-ori) was observed. To better mimic tumor microenvironment, 3D cell culture sys-
tems were employed along the traditional monolayer cultures. In 3D cultures, after 14 days of polyphenol treatments a gradual loss of spheroidal structure was observed, suggesting reduced self-renewal and enhanced differentiation. Moreover, at a molecular level, treatments resulted in a significant up-regulation of SOX17, along with the re-expres-
sion of specific thyroid markers TTF-1, TPO, and NIS, indicating a possible function restoration. In conclusion, our work aims to provide new insight into upstream regulatory
mechanisms in ATC dedifferentiation molecular processes by implementing a 3D cell culture model that more closely resembles the in vivo tumor environment. Our data highlight
the potential antitumor effect of polyphenols as differentiation-inducing agent and a promising therapeutic agent or coadjutant component to existing treatments to overcome
ATC drug resistance. Further investigation will focus on exploring the molecular upstream pathways involved in ATC dedifferentiation, as well as cytoskeletal remodeling patterns, which play a key role in the epithelial-mesenchymal transition (EMT) during the metastatic progression
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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