1,721,002 research outputs found
Label-Free Neurosurgical Pathology with Stimulated Raman Imaging: A Dataset
No full textThe goal of brain tumor surgery is to maximize tumor removal without injuring critical brain structures. Achieving this goal is challenging since it can be difficult to distinguish tumor from non-tumor tissue. While standard histopathology provides information that could assist tumor delineation, it cannot be performed iteratively during surgery as freezing, sectioning, and staining of the tissue require too much time. Stimulated Raman scattering (SRS) microscopy is a powerful label-free chemical imaging technology that enables rapid mapping of lipids and proteins within a fresh specimen. This information can be rendered into pathology-like images. Although this approach has been used to assess the density of glioma cells in murine orthotopic xenografts models and human brain tumors, tissue heterogeneity in clinical brain tumors has not yet been fully evaluated with SRS imaging. Here we profile 41 specimens resected from 12 patients with a range of brain tumors. By evaluating large-scale stimulated Raman imaging data and correlating this data with current clinical gold standard of histopathology for 4,422 fields of view, we capture many essential diagnostic hallmarks for glioma classification. Notably, in fresh tumor samples we observe additional features, not seen by conventional methods, including extensive lipid droplets within glioma cells, collagen deposition in gliosarcoma, and irregularity and disruption of myelinated fibers in areas infiltrated by oligodendroglioma cells. This dataset is released in Harvard Dataverse to foster diagnostic training and to permit additional interrogation. Our work establishes the methodology and provides a significant collection of reference images for label-free neurosurgical pathology. For more details, please refer to our paper published on Cancer Research (doi: 10.1158/0008-5472.CAN-16-0270)
Label-Free Neurosurgical Pathology with Stimulated Raman Imaging: A Dataset
No full textThe goal of brain tumor surgery is to maximize tumor removal without injuring critical brain structures. Achieving this goal is challenging since it can be difficult to distinguish tumor from non-tumor tissue. While standard histopathology provides information that could assist tumor delineation, it cannot be performed iteratively during surgery as freezing, sectioning, and staining of the tissue require too much time. Stimulated Raman scattering (SRS) microscopy is a powerful label-free chemical imaging technology that enables rapid mapping of lipids and proteins within a fresh specimen. This information can be rendered into pathology-like images. Although this approach has been used to assess the density of glioma cells in murine orthotopic xenografts models and human brain tumors, tissue heterogeneity in clinical brain tumors has not yet been fully evaluated with SRS imaging. Here we profile 41 specimens resected from 12 patients with a range of brain tumors. By evaluating large-scale stimulated Raman imaging data and correlating this data with current clinical gold standard of histopathology for 4,422 fields of view, we capture many essential diagnostic hallmarks for glioma classification. Notably, in fresh tumor samples we observe additional features, not seen by conventional methods, including extensive lipid droplets within glioma cells, collagen deposition in gliosarcoma, and irregularity and disruption of myelinated fibers in areas infiltrated by oligodendroglioma cells. This dataset is released in Harvard Dataverse to foster diagnostic training and to permit additional interrogation. Our work establishes the methodology and provides a significant collection of reference images for label-free neurosurgical pathology. For more details, please refer to our paper published on Cancer Research (doi: 10.1158/0008-5472.CAN-16-0270)
Figs. 1–4. Bledius hei, new species. 1 in A New Species of the Bledius kosempoensis Group (Coleoptera: Staphylinidae, Oxytelinae) from China1
No full textFigs. 1–4. Bledius hei, new species. 1) Aedeagus, dorsal view; 2) aedeagus, lateral view; 3) abdominal segments 9 and 10, male, dorsal view; 4) spermatheca.Published as part of Zheng, Fa-Ke, 2004, A New Species of the Bledius kosempoensis Group (Coleoptera: Staphylinidae, Oxytelinae) from China1, pp. 452-455 in The Coleopterists Bulletin 58 (3) on page 453, DOI: 10.1649/705, http://zenodo.org/record/534529
Figs. 1–4. Bledius hei, new species. 1 in A New Species of the Bledius kosempoensis Group (Coleoptera: Staphylinidae, Oxytelinae) from China1
No full textFigs. 1–4. Bledius hei, new species. 1) Aedeagus, dorsal view; 2) aedeagus, lateral view; 3) abdominal segments 9 and 10, male, dorsal view; 4) spermatheca.Published as part of Zheng, Fa-Ke, 2004, A New Species of the Bledius kosempoensis Group (Coleoptera: Staphylinidae, Oxytelinae) from China1, pp. 452-455 in The Coleopterists Bulletin 58 (3) on page 453, DOI: 10.1649/705, http://zenodo.org/record/534529
A new species of the genus Oxyporus Fabricius (Coleoptera: Staphylinidae: Oxyporinae) from Yunnan, China
No full textLi, Guo-Feng, Zhou, Yuan, Zheng, Fa-Ke (2011): A new species of the genus Oxyporus Fabricius (Coleoptera: Staphylinidae: Oxyporinae) from Yunnan, China. Zootaxa 3067: 65-68, DOI: 10.5281/zenodo.20304
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
FIGURE 1 in A new species of the genus Oxyporus Fabricius (Coleoptera: Staphylinidae: Oxyporinae) from Yunnan, China
No full textFIGURE 1. Oxyporus puerius sp. nov. A. male habitus, dorsal view. B. female habitus, dorsal view. C. male sternite 8. D. female sternite 8.Published as part of Li, Guo-Feng, Zhou, Yuan & Zheng, Fa-Ke, 2011, A new species of the genus Oxyporus Fabricius (Coleoptera: Staphylinidae: Oxyporinae) from Yunnan, China, pp. 65-68 in Zootaxa 3067 on page 66, DOI: 10.5281/zenodo.20304
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