575 research outputs found

    Spectrophotometric methods for the measurement of soil pH: A reappraisal

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    The pH of aqueous soil extracts is generallymeasured potentiometrically by glass electrode (GE). Spectrophotometric methods have also been used till around the '80s, and then they have apparently been abandoned. However, the use of microplates and spectrophotometers able to read absorbance values in them can significantly increase the speed of the data collection (saving analysis time), thus justifying their reappraisal. Three spectrophotometric methods are proposed in this work: a one-indicator (OISM), a separated three-indicator (STISM) and a mixed three-indicator (MTISM) spectrophotometric method. They are based on the addition of one or three colorimetric indicators (methyl red, bromocresol violet, and bromothymol blue) to the aqueous extract. The pHismeasured through its absorption properties in the visible region. The analysis of 60 soil samples showed that STISMand MTISMresults correlate well with the ones obtained by GE. The STISMmethod, beingmore simple and general than the other two methods, is proposed for quick routine analyses. The repeatability, reproducibility and accuracy of STISM(and of GE, for comparison)were evaluated bymeasuring several times the pH of a series of soil samples and of a certified reference soil. The standard deviations of STISM results were slightly worse than those of GE,whereas the accuracywas slightly better, indicating that STISMand GE have overall similar performances. STISM method is much faster than GE one: the analysis time saving is around 2 min per sample, and it becomes very high when hundreds of samples have to be analysed. It follows that STISM can represent an advantageous alternative to GE for rapid and accurate soil pH measurements

    Development of a Soil Health Index based on the ecological soil functions for organic carbon stabilization with application to alluvial soils of northeastern Italy.

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    There is a need to assess soil health quantitatively to assist with sustainable soil management in agroecosystems. The objective of this research was to develop a farm- level soil health index (SHI) to help identify the most sustainable management practices including C sequestration and agroecosystem resilience to climate change. For all indexing phases we used the Soil Management Assessment Framework. The SHI was created using a minimum data set (MDS) of physical, chemical and biochemical soil indicators chosen via expert opinion (EO-MDS) (24 indicators) and via the results of PCA (PCA-MDS) (16 indicators). Each observed value was converted into a score (0 to 1) by using site-specific non-linear scoring algorithms. Indicator scores were incorporated into a composite SHI which quantified the ecological performance of 5 soil functions: habitat and biodiversity, water movement and availability, filtering and buffering, nutrient cycling, physical stability and support, and long-term C stabilization. For both MDS evaluations, we assessed agroecosystems managed organically (OS) or conventionally (CS). Results from our study showed that OS had significantly higher (P< 0.05) SHI scores compared to the CS. SHI, with EO-MDS, was more efficient (P=0.028) than the PCA-MDS (P=0.039) when determining the effect of soil management practices on soil health. Non-linear transformation was a useful technique and represented soil ecological functionality effectively. Five out of six soil functions had significantly greater (P< 0.05) ecosystem performance in OS compared to CS, except for water movement and availability. The benefit for OS lead to a greater soil health status, allowing the system to be more resilient to climate change, to efficiently provide nutrients to plants, and to sustain an active soil food web for the long-term stabilization of C. The SHI may help optimize and provide extensive insight into all aspects of soil health which are intricately linked to soil-based ecological functions at the farm level

    The Spatial Representation of Angles

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    We investigated whether angle magnitude, similarly to numerical quantities\ud (i.e., the spatial-numerical association of response codes effect),\ud is associated to the side of response execution.\ud In addition, we investigated whether this association has the properties of a spatially oriented mental line,\ud since angles are taught in a right-to-left progression.\ud We tested two groups of participants: civil engineering students\ud (high familiarity with angles) and psychology students (low familiarity with angles).\ud In Experiment 1, participants were asked to judge the continuity of the angles’ arms (continuous vs. dashed).\ud Magnitude of the angles was task-irrelevant.\ud In Experiment 2, they were asked to judge whether the presented angles were smaller or larger than a right angle (90°).\ud Therefore, the angle magnitude was relevant for performing the task.\ud Overall, engineering students responded faster with their left hand to large angles and\ud with their right hand to small angles.\ud Conversely, psychology students did not show any reliable differences between left- and right-hand responses.\ud In the case of engineering students, the spatial association has a right-to-left (counter clockwise) direction,\ud suggesting the influence of education and practice on the mental representation of angle magnitude

    Interactions of prion proteins with soil

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    Prions, are proteinaceous particles recognized as the agents of a class of neurodegenerative disorders, called transmissible spongiform encephalopathies (TSE), or prion diseases. Epidemiological data suggest that TSE-contaminated environments may serve as source of infectivity, but there is no information about adsorption of prions onto soil. We carried out experiments by mixing, healthy, or scrapieinfected hamster brains homogenates with three types of soil suspended in different buffers: (i) two saline buffers, i.e., phosphate buffer solution (PBS) and CaCl2 solution; (ii) a mix of nondenaturing detergents, i.e., Triton X-100 and sodium deoxycholate (DOC) solution; (iii) a non-ionic detergent, i.e., lauryl maltoside; (iv) two anionic detergents, i.e., Sarkosyl or sodium dodecyl sulphate (SDS); and (v) a chaotropic agent, i.e., urea. The unbound prion proteins were detected in the supernatants (after centrifugation of soil suspension) by Western blotting. Results clearly demonstrate that both the no infectious (PrP C ) and infectious (PrP Sc ) forms are adsorbed by all soils. Only 1% sodium dodecylsulphate (SDS) partially impeded the association of PrP C , but not that of PrP Sc with the sandy loam soil. Agents with different interacting properties towards hydrophilic and/or hydrophobic domains failed to extract PrP Sc from sediments of soil–brain homogenate mixtures. The strong interaction of PrP Sc with soil favors the accumulation of prions in soils, especially if amended with prion-containing organic fertilizers and/or whenever TSE-affected animal carcasses, placenta, and excreta in general are buried or laid at the soil surface

    El Tlacuache Núm. 365 (2009). 365 Año 9 (2009) mayo. El Tlacuache

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    Rubén Jaramillo: las intenciones del crimen por Aura Hernández. - Jaramillo y la disputa el sentido de la vida por Flavio Barbosa. - De campesinos a obreros: una comunidad surgida entre las flores por Rafael Gutiérrez
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