1,721,129 research outputs found
Applications of two-dimensional electrophoresis technology to the study of atherosclerosis
No full textOxidative Modifications in Advanced Atherosclerotic Plaques: A Focus on In Situ Protein Sulfhydryl Group Oxidation
Full text linkDetermination of arterial proteoglycan uronic acids as their per-O-benzoylated derivatives by HPLC
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Overview of Current Proteomic Approaches for Discovery of Vascular Biomarkers of Atherosclerosis
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The levels of adenine nucleotides and pyridine coenzymes in red blood cells from the newborn, determined simultaneously by HPLC
No full textA Method for Urinary Trypsin Inhibitor (UTI) Purification Combining Anion-Exchange Chromatography Enrichment and Preparative SDS-PAGE
No full text: This chapter describes a method for the purification of urinary trypsin inhibitor (UTI), a small chondroitin sulfate proteoglycan with Ser-proteinase inhibitory activity, excreted at high levels into urine following an inflammatory condition. The method consists of two fractionation steps: an anion-exchange chromatography and a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by Coomassie Brilliant Blue G-250 gel staining. Several UTI bands are excised from gel, minced, destained, and dehydrated for extraction with SDS-containing buffer, at 60 °C for 24 h. This allows for obtaining a highly purified UTI sample useful for both structural and functional studies
Biomaterials for heart valve bioprostheses: acellular pericardium scaffold from three mammal species
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