1,720,996 research outputs found
Conserved regions in the T-DNA of different Agrobacterium rhizogenes root inducing plasmids
No full textEpigenetic factors and mitochondrial biology in yeast: A new paradigm for the study of cancer metabolism?
Full text linkBidirectional cross-talk between nuclear and mitochondrial DNA is fundamental for cell homeostasis. Epigenetic mechanisms regulate the inter-organelle communication between nucleus and mitochondria. Recent research highlights not only the retrograde activation of nuclear gene transcription in case of mitochondria dysfunction, but also the role of post-translational modifications of mitochondrial proteins in respiratory metabolism. Here we discuss some aspects and novel findings in Saccharomyces cerevisiae. In yeast, KAT-Gcn5 and DUB-Ubp8 have a role in respiration and are localized, as single proteins, into mitochondria. These findings, beside the canonical and widely known nuclear activity of SAGA complex in chromatin regulation, provide novel clues on promising aspects linking evolutionary conserved epigenetic factors to the re-programmed metabolism of cancer cells
GCN5, a yeast transcriptional coactivator, induces chromatin reconfiguration of HIS3 promoter in vivo
No full textSpecific Interaction Between a Nicotiana tabacum Nuclear Protein and the Agrobacterium rhizogenes rolB Promoter
No full textNuclear protein extracts from Nicotiana tabacum plants and cells culture were utilized in gel retardation assays and in vitro and in viva footprinting experiments in order to identify nuclear proteins, that would bind to the 5' non-coding region of the plant oncogene rolB from the Ri plasmid of Agrobacterium rhizogenes. A factor, RBF1 (Rol Binding Factor) was identified that specifically binds to a sequence located between positions -553 and -530 from the translational start codon. This DNA segment is located within a domain controlling the level of expression of rolB and it's inducibility in the non meristematic cells in the root apex (in particular protoderm and root cap). The binding sequence for RBF1 has been identified. The factor is present in extracts prepared from leaves and cells culture of untransformed N. tabacum as well as in transgenic plants, expressing rolA, rolB, and rolC (Spena et al., 1987), in apparently the same amount. Comparing the in vitro footprinting data with an in vivo high-resolution footprinting, obtained by means of a linear amplification procedure utilizing the polymerase chain reaction, we find substantially the same DNA core sequence recognized by RBF1 protein. Our results point to RBF1 as a plant endogenous factor that could be involved in controlling the level and/or tissue specificity expression of the rolB gene
Does Chlorhexidine Prevent Complications in Extractive, Periodontal, and Implant Surgery? A Systematic Review and Meta-analysis with Trial Sequential Analysis
No full textPURPOSE: This systematic review aimed to assess the effect of chlorhexidine (CHX) in preventing complications after extractive, implant, and periodontal surgery. MATERIALS AND METHODS: The PICO question set for this systematic review was: "Is the use of chlorhexidine formulations able to prevent complications (safety) in patients undergoing procedures of either oral surgery, dental implantology, or periodontology compared to treatment procedures in patients without a chlorhexidine prescription?" Once inclusion and exclusion criteria were established, a search was carried out independently by two researchers on PubMed/MEDLINE, Scopus, and Web of Science. The primary outcomes investigated were the rate of alveolar osteitis and bacteremia after surgical procedures in oral surgery. Meta-analysis and trial sequential analysis (TSA) were performed in order to evaluate the findings. RESULTS: After the selection, the 32 studies that fully met the eligibility criteria were considered in this systematic review. A meta-analysis was only possible for data obtained from studies related to extractive surgery. Meta-analysis and TSA showed a statistically significant decrease in the rate of alveolar osteitis after tooth extraction when CHX was employed compared with placebo treatments or treatments not using CHX (RR = 0.49; 95% CI: [0.40, 0.60], P < .001; I2 = 8%). Focusing on the rate of bacteremia, meta-analysis and TSA showed how the employment of CHX (RR = 0.87; 95% CI: [0.79, 0.96], P = .004; I2 = 4%) decreases the rate of bacteremia after extractive surgery. Data from the literature seem to lack in the evaluation of CHX use for the reduction of complications in periodontology and implant dentistry. CONCLUSION: This systematic review showed with a good power of evidence that CHX employment reduces alveolar osteitis and bacteremia rates after dental extractions
Quinoline Derivative MC1626, a Putative GCN5 Histone Acetyltransferase (HAT) Inhibitor, Exhinits HAT-Independent Activity against Toxoplasma gondii
No full textGoing Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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