1,720,976 research outputs found
Characterisation of a specific phycocyanin-hydrolysing protease purified from Spirulina platensis
No full textA novel protease has been identified, purified and partiallycharacterised from complete medium grown Spirulina platensis,which could be responsible for the selective proteolysis ofphycobiliproteins. It is an 80 kDa homodimeric enzyme; itsN-terminal sequence is not related to any known proteasesequence. It hydrolyses native phycocyanins in both crudeextracts and reconstructed systems with purified Allo- orC-phycocyanin. It is inactive on several native proteins, includingribulose-1,5-bisphosphate carboxylase. The twophycocyanins are degraded at different velocities since Cphycocyaninis the better substrate, in agreement with theearlier observations on the progress of the phycobilisomedisassembly. Specificity for synthetic substrates and inhibitorsstrongly suggests its assignment to the serine-proteasefamily. The enzyme, however, is insensitive to the commerciallyavailable protein inhibitors of trypsin-like proteases.[...
5' Nucleotidase: some properties of guinea pig skeletal muscle and bovine milk serum enzyme
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Proteolysis in embryogenesis of Phaseolus coccineus: possible control by ionic strength
No full textThe presence of both alkaline and acidic proteinase activities has been evidenced in the suspensor of 11 mm long seeds of Phaseolus coccineus.
The acidic activity is mainly localized in the basal giant cells and is regulated by low molecular weight thermostable inhibitors. There is evidence from in vitro experiments that ionic strength may regulate the activity
Evidence for the inducibility of the phycocyaninase in the cyanobacterium Arthrospira platensis (Spirulina Platensis)
No full textGoing Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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