1,720,970 research outputs found
On the presence of a novel-described cell side population enriched in germ stem cell activity in the mouse testis
No full textPhoenix, Arizona – US
Immunohistochemical localization of peroxisomal enzymes during rat embryonic development
No full textPeroxisomes are cytoplasmic organelles involved in a variety of metabolic pathways. Thus far, the morphological and biochemical features of peroxisomes have been extensively characterized in adult tissues. However, the existence of congenital peroxisomal disorders, primarily affecting tissue differentiation, emphasizes the importance of these organelles in the early stages of organogenesis. We investigated the occurrence and tissue distribution of three peroxisomal enzymes in rat embryos at various developmental stages. By means of a highly sensitive biotinyl-tyramide protocol, catalase, acyl-CoA oxidase, and ketoacyl-CoA thiolase were detected in embryonic tissues where peroxisomes had not thus far been recognized, i.e., adrenal and pancreatic parenchyma, choroid plexus, neuroblasts of cranial and spinal ganglia and myenteric plexus, and chondroblasts of certain skeletal structures. In other tissues, i.e., gut epithelium and neuroblasts of some CNS areas, they were identified earlier than previously. In select CNS areas, ultrastructural catalase cytochemistry allowed identification of actively proliferating organelles at early developmental stages in several cell types. Our data show that in most organs maturation of peroxisomes parallels the acquirement of specific functions, mainly related to lipid metabolism, thus supporting an involvement of the organelles in tissue differentiation
Immunohistochemical localization of peroxisomal enzymes during rat embryonic development
No full textPeroxisomes are cytoplasmic organelles involved in a variety of metabolic
pathways. Thus far, the morphological and biochemical features of peroxisomes have been
extensively characterized in adult tissues. However, the existence of congenital peroxisomal
disorders, primarily affecting tissue differentiation, emphasizes the importance of
these organelles in the early stages of organogenesis. We investigated the occurrence and
tissue distribution of three peroxisomal enzymes in rat embryos at various developmental
stages. By means of a highly sensitive biotinyl–tyramide protocol, catalase, acyl-CoA oxidase,
and ketoacyl-CoA thiolase were detected in embryonic tissues where peroxisomes
had not thus far been recognized, i.e., adrenal and pancreatic parenchyma, choroid plexus,
neuroblasts of cranial and spinal ganglia and myenteric plexus, and chondroblasts of certain
skeletal structures. In other tissues, i.e., gut epithelium and neuroblasts of some CNS
areas, they were identified earlier than previously. In select CNS areas, ultrastructural catalase
cytochemistry allowed identification of actively proliferating organelles at early developmental
stages in several cell types. Our data show that in most organs maturation of
peroxisomes parallels the acquirement of specific functions, mainly related to lipid metabolism,
thus supporting an involvement of the organelles in tissue differentiation
Spermatogonial stem cells display a side-population phenotype
No full textXIIIth European Testis Workshop, Dunblane, Scotland, 24-28th April 200
Expression of the adaptor protein m-Numb in mouse male germ cells
No full textJCR Subject Category: Reproductive Biology IF= 3.1
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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