1,720,975 research outputs found
Sulle tracce del Sale. CD-Rom multimediale
No full textNell'ambito dell’iniziativa "Sulle tracce del Sale" organizzata dal Museo "Gemma 1786" del Museo del Dipartimento di Scienze della Terra dell'Università di Modena e Reggio Emilia, è stato realizzato il CD-Rom multimediale "Sulle tracce del Sale" in collaborazione con l'ISA Venturi di Modena.Il CD-Rom, che descrive gli aspetti storici, sociali e culturali di questo bene prezioso come presentati all'interno del percorso espositivo realizzato presso il Museo "Gemma 1786", è stato distribuito come gadget ai visitatori la mostra ed è scaricabile dal sito web del Progetto TED della Provincia di Modena:http://ted.scuole.provincia.modena.it/progetti/sulle_tracce_del_sale/index.ht
Avian metapneumovirus RT-nested-PCR: A novel false positive reducing inactivated control virus with potential applications to other RNA viruses and real time methods.
No full textUsing reverse genetics, an avian metapneumovirus (AMPV) was modified for use as a positive control for
validating all stages of a popular established RT-nested PCR, used in the detection of the two major AMPV
subtypes (A and B). Resultant amplicons were of increased size and clearly distinguishable from those
arising from unmodified virus, thus allowing false positive bands, due to control virus contamination of
test samples, to be identified readily. Absorption of the control virus onto filter paper and subsequent
microwave irradiation removed all infectivity while its function as an efficient RT-nested-PCR template
was unaffected. Identical amplicons were produced after storage for one year. The modified virus is likely
to have application as an internal standard as well as in real time methods. Additions to AMPV of RNA
from other RNA viruses, including hazardous examples such HIV and influenza, are likely to yield similar
safe RT-PCR controls
Utilizzo di Reverse Genetics per la messa a punto di un controllo positivo per la rilevazione e distinzione, mediante RT Nested PCR, di Metapneumovirus aviare sottotipo A e B
No full textQuesto lavoro descrive l’utilizzo di metodiche di Reverse Genetics per la produzione di un virus geneticamente modificato in grado di generare nella RT Nested PCR standard precedentemente descritta, amplificati di dimensioni maggiori rispetto a quelli generati da virus non modificati. Tale virus, impiegato come controllo positivo, rende possibile evidenziare immediatamente eventuali contaminazioni
Unexpected pattern in expression of AMPV proteins: possible explanations and implications
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
USE OF REVERSE GENETICS TO DEVELOP A POSITIVE CONTROL VIRUS FOR RT-NESTED PCR DETECTION OF SUBTYPE A AND B AVIAN METAPNEUMOVIRUS (AMPV)
No full textA standardized RT-nested PCR of high sensitivity has been in use in our laboratory for some time to detect avian metapneumovirus subtype A and B. Until now we have avoided the use of positive control viruses (CV) because of the risk of contamination
leading to sample false positives. The paper describes the production and testing of a modified virus which in our standard PCR produces RT-nested PCR amplicons of increased sizes compared to those generated from unmodified viruses, thus enabling cross contamination to the sample tested to be instantly detectable. A DNA copy of an AMPV subtype A genome was modified by site directed mutagenesis to introduce the subtype B G gene primer sequence (G9+B) at the equivalent position in the
subtype A G gene. To increase amplicon sizes, an insertion was introduced between binding sites for the opposing primer pairs (outer pair G1+ and G6-, inner pairs G8+A and G5- or G9+B and G5-). PCR from the modified DNA produced nested amplicons
of 463 and 556 bp compared to 268 for unmodified subtype A and 361 for unmodified subtype B viruses. Using reverse genetics the modified DNA generated a virus which, after RNA extraction, gave the same 463 and 556 bp amplicons in RT-nested PCRs. For further convenience virus was absorbed onto filter paper, dried and
inactivated by microwave treatment, then stored in flip top tubes. RNA extracted from papers were henceforth used as reliable, efficient and distinguishable RT-nested PCR positive controls
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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