1,720,989 research outputs found
Nuclear factor kB (NF-kB), nuclear factor interleukin-6 (NF-IL6 or C/EBP b) and nuclear factor interleukin-& (NFIL6 or C/EBPd) are not sufficient to activate the endogenous interleukin-6 gene in the human breast carcinoma cell line MCF-7: coparative analysis with MDA-MB-231 cells, an interleukin-6 expressing human breast carcinoma cell line
No full textProtein synthesis inhibitors cycloeximide and anisomycin induce inerleukin-& gene expression and activate transcription factors Nuclear factor-kB
No full textNuclear factor kB (NF-kB), nuclear factor interleukin-6 (NF-IL6 or C/EBP b) and nuclear factor interleukin-& (NFIL6 or C/EBPd) are not sufficient to activate the endogenous interleukin-6 gene in the human breast carcinoma cell line MCF-7: coparative analysis with MDA-MB-231 cells, an interleukin-6 expressing human breast carcinoma cell line
No full textCloning and functional characterization of the human heterogeneous nuclear ribonucleoprotein type I promoter.
No full textWe have cloned and functionally characterized a portion of the human hnRNP I (heterogeneous nuclear ribonucleoprotein type I) gene containing the promoter elements. HnRNP I is an alternative splicing modulator of tissue-specific transcripts that is expressed in three different isoforms. The DNA sequence at the transcription start site, identified by 5 ' -rapid amplification of cDNA ends, shows a high 'GC' content, lacks canonical TATA sequences and contains multiple putative Sp I and NF1 transcription factor-binding sites, a GATA box and a CAAT box. By means of a chloramphenicol acetyltransferase reporter construct and deletion analyses, we have identified two regions between -770 bp and -206 bp that had a positive effect on expression activity in HeLa cells
Protein synthesis inhibitors cycloeximide and anisomycin induce inerleukin-& gene expression and activate transcription factors Nuclear factor-kB
No full textActivation of the Interleukin-6 promoter by a dominant negative mutant of c-Jun
No full textThe human IL-6 promoter contains multiple regulatory elements such as those binding transcription factors belonging to the NF-kB (–75/–63), C/EBP (-158/-145 and -87/–76) and AP-1 (–283/-277) families. Herein, we report that ectopic expression of c-Jun, C/EBPdelta, and the p65 subunit of NF-kB synergistically activates an IL-6 promoter construct containing only a TATA box and an kB binding site. These results suggest that interactions among NF-kB, C/EBP, and AP-1, which are all activated by the most powerful physiological inducers of the IL-6 gene, namely TNF-alpha and IL-1, may be crucial for maximal activation of the IL-6 promoter in response to the two cytokines. Furthermore, we show that a mutated form of c-Jun lacking the transactivation domain (TAM-67) was a much stronger activator of the IL-6 promoter than c-Jun. In combination with p65 and/or C/EBPdelta, TAM-67 also synergistically activated the IL-6 promoter, while it inhibited TNF-alpha induced AP-1 activity directing an AP-1-responsive reporter plasmid. Lastly, EMSA results strongly suggest the formation of complexes between p65, C/EBPdelta, and/or c-Jun or TAM-67 on the kB site supporting the idea that the functional synergism is determined by a physical interaction. These data provide new insight into the molecular mechanisms regulating the formation of the transcription complex responsible for IL-6 promoter activation
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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