1,720,974 research outputs found
Isolasi dan Identifikasi Aeromonas hydrophila pada Ikan Lele (Clarias gariepinus) Pertambakan Muara Jambi, Provinsi Jambi
Get PDFTujuan penelitian ini adalah untuk mengidentifikasi bakteri Aeromonas hydrophila pada ikan lele dengan gejala klinis pada kolam bioflok di Kabupaten Muara Jambi, Provinsi Jambi. Bakteri diisolasi dari 35 ekor ikan lele yang memiliki gejala infeksi A. hydrophila. Pengambilan sampel dilakukan Purposive random sampling. Identifikasi dilakukan uji biokimia berdasarkan Standar Nasional Indonesia SNI 7303.1-2015 dan dilakukan uji konfirmasi metode PCR taget gen aerA. Hasil penelitian menujukkan bahwa sebanyak 35 ekor ikan terisolasi 12 isolat bakteri A. hydrophila pada uji biokimia. Namun pada pengujian PCR pada agarose gel 0.8% menujukkan 8 Isolat posistif gen aerA pada 309 bp. Hasil yang berbeda dapat diakibatkan karena adanya positif palsu pada saat uji biokimia. Hasil identifikasi A. hydrophila dengan metode PCR dapat digunakan hasil yang lebih akurat dan waktu uji lebih cepat dibandingkan dengan uji biokimia. Identifikasi yang cepat dapat mempermudah dalam pencegahan infeksi lebih efektif dan cepat
Pemakaian Duddingtonia flagrans dan Saccharomyces cerevisiae dalam Mereduksi Larva Infektif Haemonchus contortus (THE STUDY OF DUDDINGTONIA FLAGRANS AND SACCHAROMYCES CEREVISIAE USE ON REDUCING OF INFECTIVE HAEMONCHUS CONTORTUS LARVAE)
No full textThe use of Duddingtonia flagrans as the biological control of nomatode infections has been widelyreported. However, no report is available on the use of yeast Saccharomyces cerviciacae for such purpose.The aim of this study was to ivestigate the use of both fungi to reduce the number of Heamoncus contortusinfective larvae. Agar and fecal media containing the spore of the fungi was inoculated with infected H.contortus larvae (3rd stage). Fecal media containing the fungi was prepared by oral inoculation of sheepwith liquid containing 106, 107 spores of D. flagrans, and 106, 107 spores of D. flagrans, and 106, 1012 sporesof S. cerviciae. The number of larvae trapped in the fungi was counted. The result showed both fungi wereable to reduce the number of infective lave. However, for D. flagrans, beside it able to kill the larvae, it alsoable to trap the larva which did not occur in S. cerviceae. The combination of both fungi can be used to reduceof the number of invected H. contortus larvae
Penggunaan Imunostik sebagai Uji Serologi untuk Deteksi Brucella abortus pada Sapi (APPLICATION IMMUNOSTICK ASSAY FOR SEROLOGICAL TEST BRUCELLA ABORTUS IN BOVINE)
Get PDFSerological test is one of diagnostic method to detect pathogenicity brucella. Several methods are being improving such as Rose Bengal Test (RBT), Complement Fixation Test (CFT) dan Enzyme-linked Immunosorbent Assay (ELISA). Immunostick has an accuracy equivalent to ELISA and is easy to apply in the field so it is possible to be applied as a rapid test for brucellosis detection. The study aim was to know sensitivity and specificity of immunostick that were used to detecte antibody Brucella abortus using commercial antigens of B. abortus Strain 19 (S19) and B. abortus Strain 99 (S99). The test have compared with ELISA. The tests were conducted in two stages, namely (i) immunostick ability to detect antibodies in seropositive and seronegative serum, and (ii) the immunostick result were compared with ELISA result in serum grup that were be know and unknown status. A total of 250 serums were examined and result indicated that immunostick can be detect B. abortus antibodies in cattle serum with sensitivity 100%. Immunostick specifity were 45,45% for B. abortus S99(1) antigen; 78,79% for B. abortus S99(2) antigen and 51,52% for B. abortus S19 antigen. When the test compared with ELISA, the sensitivity 82,86% and the spesifity were 52,31% for B. abortus S99(1) antigen; 93,54% and 79,71 for B. abortus S99(2) antigen and 82,86% and 58,46% for B. abortus S19 antigen
Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVER
No full textA study to detect Coxiella burnetii, an intracellular bacterium causing Q fever in human and livestock animals, was carried out in several ruminants in Bogor and Bali. The methods used for the detection was Nested-Polymerase Chain Reaction (Nested-PCR). Two pairs of primers, the first (OMP1 and OMP2) and the second (OMP3 and OMP4) were used to detect the genomic sequences and the conserved specific sequences of Coxiella burnetii, respectively. Organ samples such as liver and lung from 410 livestock ruminants, consisting of cattle (245 samples), sheep (105 samples) and goats (60 samples) were collected from several slaughter houses in Bogor and Bali. As many as 15 (6.12%) out of 245 cattle, 6 (5.71%) out of 105 sheep and none from goat were infected by Coxiella burnetii. Interestingly, 3 out of 15 infected cattle were Bali cattle. The results clearly indicate that Q fever is likely to be widespread among ruminant animals in Indonesia
FORMULASI SUSU BERKHASIAT ANTI FLU BURUNG DAN ANTI DIARE; PRODUKSI IMUNOGLOBULIN Y DALAM BERBAGAI BENTUK
Get PDFThe ultimate goal of fresh milk formulations are efficacious against-bird flu and diarrhea (against Escherichia coli and Salmonella enteritidis). Outputs generated in this first year of immunoglobulin Y anti-bird flu and anti-diarrhea in various forms of spray dry yolk, dry freeze egg yolks, and pure extracts. Ig Y that collected is located in the egg yolks and tested positive for H5N1 virus, E. coli and S. enteritidis. Production of Ig Y begins with the treatment in the form of vaccination in laying hens 4 (four) times during the 4 (four) weeks. The vaccines were used in the form of H5N1 in the first and third week, while E. coli and S. enteritidis used every week. Serum and yolk was collected and checked for the presence of anti-Ig Y was the third agent since the first week after the last vaccination. Inspection method for E. coli and S. Enteritidis is the order Presipitation Gel Test (AGPT) while the H5N1 method Haemagglutination Inhibition (HI). The results give a positive result against all three antigens, but in a different week. This situation is common given the immunity of each chicken is different depending on race, age and environmen
Peran dan arti pengendalian penyakit hewan akuatik (aquatic animals) untuk kesejahteraan masyarakat (3 September 2005) / oleh Fachriyan Hasmi Pasaribu
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Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Characterization and molecular detection of pathogenicity and antibiotic resistance genes in Vibrio parahaemolyticus isolated from Pacific white shrimp
No full textABSTRACT
This study aimed to conduct the characterization and molecular detection of the pathogenicity and antibiotic-resistance genes in Vibrio parahaemolyticus, as the causative agent of vibriosis in Pacific white shrimp. The V. parahaemolyticus isolates were collected from the shrimp’s hepatopancreas, before biochemical test and polymerase chain reaction (PCR) sequencing of the 16S rRNA gene confirmation. The hemolysis test and PCR were applied to detect the presence of virulence genes, namely toxR, thermostable direct haemolysin (tdh), and tdh-related haemolysin (trh). The Kirby-Bauer method was used for characterizing the resistance patterns against ampicillin (AMP), tetracycline (TET), cyprofloxacin (CIP), enrofloxacin (ENR), and chloramphenicol (CHL). The biochemical tests and PCR-16SrRNA gene sequencing confirmed that 12 isolates belonged to V. parahaemolyticus that were further verified by amplification of the toxR gene in 382 bp (100% of the isolates). The alpha hemolysis activity was also confirmed by the amplicon of 199 bp in all isolates. All V. parahaemolyticus isolates showed their resistance to AMP and 42% of the isolates were TET-resistant. However, no resistance was shown to CIP, ENR, and CHL. The PCR-based analysis resulted a detectable resistance gene of ampC (42% of the isolates) and tetB (83% of the isolates).
Keywords: antibiotics, shrimp, resistance, virulency, Vibrio parahaemolyticus
ABSTRAK
Penelitian ini bertujuan untuk melakukan karakterisasi dan deteksi molekular dari gen patogenisitas dan resistansi antibiotik pada Vibrio parahaemolyticus, agen penyebab vibriosis pada udang vaname. Isolat V. parahaemolyticus dikoleksi dari hepatopankreas, diuji secara biokimiawi dan selanjutnya dikonfirmasi dengan polymerase chain reaction (PCR)-sekuensing dari gen 16S rRNA. Tes hemolisis dan metode PCR diterapkan untuk mendeteksi keberadaan gen virulensi toxR, thermostable direct haemolysin (tdh) and tdh-related haemolysin (trh). Metode Kirby Bauer digunakan untuk karakterisasi pola resistansi terhadap ampisilin (AMP), tetrasiklin (TET), kloramfenikol (CHL), siprofloksasin (CIP) dan enrofloksasin (ENR). Uji biokimia dan sekuensing gen PCR-16SrRNA memastikan bahwa 12 isolat adalah V. parahaemolyticus yang selanjutnya diverifikasi dengan amplifikasi gen toxR berukuran 382 bp (100% isolat). Aktivitas alfa hemolisis juga dikonfirmasi dengan amplikon PCR (199 bp) di semua isolat. Seluruh isolat V. parahaemolyticus menunjukkan resistansinya terhadap AMP, 42% resistan TET, tidak ada resistansi yang ditunjukkan pada CIP, ENR dan CHL. Analisis berbasis PCR menghasilkan gen resistan yang terdeteksi dari gen ampC (42% isolat) dan gen tetB (83% isolat).
Kata kunci: Antibiotik, udang, resistansi, virulensi, Vibrio parahaemolyticu
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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