176 research outputs found
Immunolocalization of estrogen and progesterone receptors in ewe mammary glands
No full textAbstract
Immunopresence of estrogen receptor α (ERα), β (ERβ) and progesterone receptor (PR) were examined in the ewe mammary gland from prepubertal stage to involution. Immunolocalization of ERα revealed a strong positive staining in nuclei of cells composing terminal ductular units (TDUs) in prepubertal ewes. A mild immunoreactivity was identified in early lactating gland. During late lactation immunoreactive product to ERα was observed in the cytoplasm of glandular epithelial cells in all alveoli. In mammary glands at involution ERα positivity was clearly nuclear, with weak to moderate cytoplasmic staining. Cytoplasmic strong immunostaining for ERβ was detected in cells of TDUs, whereas some stromal cells exhibited nuclear staining. A nuclear ERβ immunostaining was observed at early lactation, instead during late lactation, the positivity for ERβ showed only a moderate cytoplasmic distribution. At involution, ERβ positivity was very moderate and detected just in the cytoplasm of shrunken alveoli. Scattered nuclear staining of PR was observed just in mammary glands at early lactation. These results showed that in the mammary glands of sheep both estrogen receptor isoforms were displayed during lactation cycle and that PR appeared just at early lactation, reflecting their regulatory role in alveolar cells
Corrigendum to “Presence and function of kisspeptin/KISS1R system in swine ovarian follicles” (Theriogenology (2018) 115 (1–8), (S0093691X1830147X), (10.1016/j.theriogenology.2018.04.006))
No full textThe authors regret the following changes to the author group G. Basinia, F. Grassellia, S. Bussolatia, R. Ciccimarraa, M. Maranesib, A. Bufalarib, C. Dall'Agliob, F. Parilloc,#, M. Zeranib,c,*. a Dipartimento di Scienze Mediche Veterinarie, Università di Parma, 43126 Parma, Italy. b Dipartimento di Medicina Veterinaria, Università di Perugia, 06126 Perugia Italy. c Scuola di Bioscienze e Medicina Veterinaria, Università di Camerino, 62024 Matelica Italy. # Deceased. * Corresponding author: tel.: +39 0755857642; fax +39 0755857654. E-mail address: [email protected] (M. Zerani). And to the acknowledgements and figures
Glycocomposition of the apocrine interdigital gland secretions in the fallow deer (Dama dama)
No full textThe secretions of the tubular interdigital glands were investigated by conventional (PAS, Alcian-Blue at different pH, Low and High Iron Diamine) and lectin (Con-A, UEA-I, LTA, WGA, GSA-II, GSA-IB4, SBA, PNA, ECA, DBA, MAL-II and SNA) histochemical methods in adult males and females of different age of fallow deer during the breeding season. Sialidase digestion and deacetylation pre-treatment were also employed in conjunction with lectin histochemistry. The glandular epithelium consisted of a single layer of low columnar cells with typical apical protrusions. No substantial differences of the above histochemical staining in relation to sex and age were observed. Conventional histochemical staining revealed that the interdigital glands secreted neutral glycoproteins whereas acidic glycocomponents did not seem to be present. Lectin histochemical technique allowed us to disclose a great heterogeneity of glycoproteins with N- and O-linked oligosaccharides containing a-D-Man/a-D-Glc, GlcNAc, a-Fuc, terminal b-D-Gal-(1-3)-D-GalNAc, -D-Gal-(1-4)-D-GlcNAc, a-Gal and b-GalNAc residues. b-GalNAc and disaccharide b-D-Gal-(1-3)-D-GalNAc were also found as subterminal to sialyl moieties. The lack of sexual and age-related differences in the glucidic content of the glandular secretions seems to indicate that the glycoderivatives may play only an accessory role in the production of odoriferous signals in fallow deer
Characterization of glycoconjugates in the secretory epithelium of equine ampulla ductus deferentis
No full textThe present work was undertaken to
determine the glycoconjugates secreted by the
epithelium of the equine ampulla ductus deferentis,
using conventional (PAS, AB pH 2.5, AB pH 1.0) and
lectin histochemical procedures in conjunction with
enzymatic digestion and chemical treatment. The
presence of abundant apical cytoplasmic blebs suggests
that the equine ampulla secretes its products mainly in
an apocrine manner. Glandular cells secrete neutral and
acidic sialylated glycoconjugates as revealed by
conventional histochemical procedures. Lectin
histochemistry helped us to discover the following
histological positive sites: the mucosal cells, the
glandular epithelial cells, the apical cytoplasmic blebs
and the basal cells. The ampullary secretions contained
both glycoproteic material (revealed by Con-A-, LCA-,
GSA-II-, WGA-, RCA-I- positivity) and sialomucins
(evidenced by the reactivity of GSA-II, SBA, PNA and
RCA-I after sialidase digestion) having different
functional roles. The mucosal cells reacted with Con-A,
LCA, and also with sialidase/GSA-II-, SBA-, PNA-, and
RCA-I sequences, contributing to the chemical
heterogeneity of ampullary secretions. DBA lectin was a
specific marker for basal cells.
The results obtained were compared with our
previous findings regarding the differences in the lectin
binding pattern of the plasma-membrane of equine
sperm collected from epididymal cauda and the ampulla
ductis deferentis. Our results support other studies that
indicate that ampullary secretions are involved in
altering the plasma-membrane glycoconjugates of
spermatozoa, contributing to their maturation
A NOVEL APPROACH FOR IMPLEMENTING OF A LOG-SIGMOID FUNCTION ON A FPGA DEVICE USING THE SFLOAT24 MATH LIBRARY – AN MODELLING –
No full textAn Application in Bank Credit Risk Management System employing a BP Neural Network based on sfloat24 custom math library using a low cost FPGA device
No full text
A Current Hysteresis Controller for Reduction of Switching Losses in a Full-Bridge Inverter ─ FPGA implementation by using a custom developed 24 bit Floating Point Math Library ─
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