1,720,959 research outputs found
MESENCHYMAL STEM CELLS FROM HUMAN ADIPOSE TISSUE:CHARACTERIZATION AND IMMUNOMODULATORY PROPERTIES OF TISSUE AND CELLULAR PRODUCT
Full text linkHuman adipose tissue – derived stem cells (hADSCs) are routinely isolated from the stromal vascular fraction of homogenized adipose tissue.
In this study a mechanical, non-enzymatic method to obtain mesenchymal stem cells from the human subcutaneous adipose tissue, by use of the Lipogems® device has been set.
This instrument allows three micro-fragmentation steps of the lipoaspirate adipose tissue, in order to increase the stem cells bioavailability, residents in the stromal vascular fraction of the tissue, diminishing oil and blood residues.
The structural composition of the Lipogems® product is comparable to the original lipoaspirate adipose tissue, but differently, this mechanical procedure activates the expression of antigens such as β-tubulin III.
The Lipogems® product could be processed to obtain hADSCs with different approaches, including the classical enzymatic treatment, a single centrifugation or a directly tissue culture, although the yield is different and it is associated to the used method. Freshly isolated they display surface markers that differ from those of cultured hADSCs.
The hADSCs derived from Lipogems® product have been systematically characterized for growth features, phenotype and multipotent differentiation potential. They fulfill the definition of mesenchymal stem cells provided by the International Society of Cell Theraphy (ISCT).
Notably, in basal growth condition Lipogems®- derived stem cells express at high percentages typical neural markers, such as GFAP, O4 and β-tubulin III. The EPO Receptor (EPOR) is express when these cells grown in SCM medium. All these findings provide evidence that micro-fragmentation of lipoaspirate adipose tissue change some molecular features in the tissue resident cells so that hADSCs obtained in this condition become more plastic and adaptive to the extracellular signals.
The immunomodulatory properties of Lipogems®- derived stem cells have been evaluated by means of anti CD3 assay of co-culture with CD3+ T lymphocytes. They show a strongly suppressive effect on the T cells proliferation, maintaining a consistent T regulatory population
Death-resistant neural progenitors yield mostly neurons : an erythropoietin-dependent process
No full textPhenotype plasticity of mesenchymal stem cells from LipostemTM-derived human adipose tissue
No full textAdipose tissue is derived from the embryonic mesoderm, and the human adipose tissue derived stem cells (hADSCS) are multipotent stem cells able to differentiate into different cell lineages such as bone, muscle and neural cells. This study is aimed at isolation and in vitro and in vivo characterization of hADSCs obtained from adipose tissue without enzimatic digestion. Adipose tissue was fragmented by means of LipostemTM. This is a system the allows the fragmentation of adipose tissue into clusters as small as 500μm. The reduced particle size of adipose tissue enriches he content in available stem cells that creep out of the tissue tissue and within 7 days are ready for the first passage. hADSCs are 100% positive to surface markers tipical of mesenchymal stem cells such as CD44, CD73, CD90, CD105, CD146 and CD166. About 50% of these express also CD34 and CD45. The phenotypical expression of hADSCs is sensitive to growth medium and to adhesion specific substrates. For instance these cells express Vimentin, Nestin, GFAP and TUJ1, but the switch to different adhesion substrate can modify such expression. For instance the use of MatrigelTM eliminates the expression of GFAP. Thus in addiction to changes in morphology these stem cells change intracellular phenotype according to medium and surface substrates. All hADSCs espresse the potein O4 typical oligodendrocyte precursors when these cells were grown in presence of Human Neural medium. In conclusion our study shows that hADSCs obtained from adipose tissue by means of LipostemTM grow nicely in vitro, show typical mesenchyme surface proteins, and modify their phenotype accrding to growth medium and surface substrates
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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