3,224 research outputs found

    Bioconversion of D-xylose to xylitol by Debaryomyces hansenii UFV-170: Product formation versus growth

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    The relationship between D-xylose-to-xylitol conversion and cell growth was investigated in the yeast Debaryomyces hansenii UFV-170

    Xylose metabolism in Debaryomyces hansenii UFV-170. Effect of the specific oxygen uptake rate

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    The new yeast Debaryomyces hansenii UFV-170 was tested in this work in batch experiments under variable oxygenation conditions

    Efeito da adubação nitrogenada na incidência do pulgão Cerosipha forbesi (Weed, 1889) em morangueiro Fragaria vesca L.

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    Com o objetivo de examinar o efeito da adubação nitrogenada na incidência do pulgao Cerosipha forbesi, foi instalado em maio de 1991, no campo experimental da EMBRAPA - CNPDA, em Jaguariúna,SP, um experimento em blocos causalizados com 4 repetições e 3 tratamentos, sendo T1, testemunha com 0 grama de sulfato de amônia por parcela; T2 com aplicação de 200 gramas de sulfato de amônia por parcela e T3 com 2000 gramas de sulfato de amônia por parcela. Cada parcela constou 4 fileiras com 10 plantas cada, sendo a parcela útil as duas fileiras centrais menos as plantas das extremidades. A avaliação foi feita em dezembro de 1991, tomando-se ao acaso 5 plantas da parcela útil, nas quais foram constados os pulgões encontrados. Os dados originais receberam transformação de arco-seno raiz quadrada de y. A maior incidência de pulgões foi verificada no tratamento testemunha - (7,712), seguido de tratamento T3 (4,249) e T2 (2,607). Não Houve diferença significativa pelo teste de Ducan ao nivel de 5% entre os tratamentos T1 e t2. Nesse experimento nao se verificou aumento da incidência do pulgão com o aumento da dose de nitrigenio

    Influence of xylose concentration and glucose/xylose ratio on fermentation by Debaryomyces hansenii UFV-170

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    The purpose of this work was to investigate the effects of xylose concentration (So = 50.0 to 275.0 g L-1) and glucose/xylose ratio (Sg/Sx = 0.09, 0.25 and 0.50) on the fermentative behavior of Debaryomyces hansenii UFV-170. The maximum cell productions expressed as cell dry weight were 40.28 g L-1 on pure xylose (Sx=106.30 g L-1) and 31.29 g L-1 on the glucose/xylose mixture (Sg = 5.0 g.L-1/Sx = 50.0 g L-1). The highest xylitol concentration (PXyt=130.43 g L-1) was obtained at Sx = 190.0 g L-1 corresponding to a xylitol yield (YXyt/S) of 0.77 g g-a, volumetric productivity (QXyt) of 0.54 g L-1 h-1 and specific productivity (qXyt) of 0.16 mmol g-1 h-1. High xylose levels (Sx≥187.0 g L-1) did not increase xylitol accumulation, but stimulated ethanol production. An increase in Sg/Sx ratio from 0.09 to 0.25 enhanced xylitol production at Sx = 50.0 g L-1, but not at Sx = 100.0 g L-1. In the simultaneous presence of glucose and xylose, PXyt=36.54 g L-1 was observed for Sg = 14.0 g L-1/Sx 50.0 g L-1 with YXyt/S=0.72 g g-1, QXyt=0.36 g L-1 h-1 and qXyt=0.08 mmol g-1 h-1. At the evaluated glucose/xylose ratio, glycerol and ethanol were formed as by-product

    Nos passos de Antonieta: escrever uma vida

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    Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão, Programa de Pós-Graduação em Literatura, Florianópolis, 2010Nos Passos de Antonieta: escrever uma vida propõe mostrar a trajetória de Antonieta de Barros, sua vida e sua obra. Reflete e retrata, dentro do contexto 1901-1952, em Santa Catarina, a atuação da professora, da escritora, mulher e negra na política e literatura, vislumbrando a importância de seus escritos: crônicas, discursos e projetos, além da influência de sua idéias no pensamento sócio-político-educacional na Ilha de Santa Catarina do sec.XX. Utiliza-se de fotos, documentos, imagens, meios eletrônicos e menções em livros e jornais, os vestígios e rastros, para contar os passos de Antonieta. Um resgate histórico-cultural da escrita feminina na Literatura catarinense, verificando, a atuação da professora-escritora em todas as instâncias da vida pública

    Influence of temperature and pH on xylitol production from xylose by Debaryomyces hansenii UFV-170

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    The effects of temperature and starting pH on xylose-to-xylitol bioconversion by the new yeast isolate Debaryomyces hansenii UFV-170 were investigated

    Use of response surface methodology to predict optimal conditions of Kluyveromyces lactis permeabilization by a physical method

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    Kluyveromyces lactis is an important commercial source of β-galactosidase, an enzyme of major concern for dairy industry. Among the methods for β-galactosidase recovery, cell permeabilization is the best alternative, but it is influenced by a number of operating conditions that need to be optimized. Aim of this study was to determine the best experimental conditions for yeast cells permeabilization by physical disruption with glass beads. Influence of glass beads and dry biomass amounts and process time was investigated varying these independent variables according to a central composite rotatable experimental design. A third order polynomial equation was fitted to the experimental data of permeabilized cells β-galactosidase activity, and the Response Surface Methodology employed for optimization. Optimum permeabilization conditions (0.90 g glass beads, 2.00 g dry biomass and 8.0 min of treatment) ensured a maximum β-galactosidase activity (6,434 mmol L-1 oNP min-1 g-1) in reasonable agreement with the yield reproduced under the same conditions (5,585.40 mmol L-1 oNP min-1 g-1)

    Anatomia dos segmentos foliares de Mauritia flexuosa L. f. (Arecaceae) em três fases de desenvolvimento

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    Apresentam-se dados anatômicos dos segmentos foliares de Mauritia flexuosa L. f. (Arecaceae) em três fases de desenvolvimento (jovem, intermediária e adulta). Os segmentos foliares foram analisados em toda a extensão do limbo. As células das epidermes adaxial e abaxial evidenciam-se com paredes sinuosas, retagulares e orientadas longitudinalmente ao eixo do segmento foliar com acentuada presença de corpos de sílica esférico espinulosos. Os segmentos foliares de M. flexuosa são anfiestomáticos com estômatos do tipo tetracíticos. Em secção transversal a epiderme é uniestratificada com estômatos e câmara subestomática ampla. O mesofilo é dorsiventral verificando-se uma camada hipodermal intercalada por feixes de fibras não vasculares e uma a duas camadas de parênquima paliçádico. Os feixes vasculares são envolvidos por endoderme e fibras pericíclicas, formando a bainha de feixe. Os resultados obtidos não demonstraram variações expressivas entre as três fases de desenvolvimento e os caracteres encontrados parecem ser comuns para outras palmeiras

    Samba, deep water, and poles: a framework for exercise prescription in Parkinson's disease

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    Parkinson's disease is a brain disorder that leads to tremor, slowness, muscle stiffness, and other movement disorders. The benefits of exercise for reducing disability in individuals with Parkinson’s disease are numerous. However, not much is known about the designing and prescription of exercise in neurodegenerative diseases. A brief review and indications for exercise prescription and evaluation are discussed throughout. In this scoping review, we specifically aimed to describe the applicability of walking tests (6-min/10-m) for the prescription of exercise in individuals with Parkinson’s disease and to propose training (undulating periodized) designs in three exercise modalities, Brazilian dance rhythms (Samba and Forró), deep-water exercises, and Nordic walking. These training models and evaluation methods may assist coaches and therapists in organizing exercise programs adequate to people with Parkinson’s disease, and are essential steps toward a comprehensive and more detailed understanding of the training loads in motor disorders and disease states

    Effects of nordicwalking on gait symmetry in mild Parkinson's disease

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    Individuals with Parkinson's disease (PD) have gait asymmetries, and exercise therapy may reduce the differences between more and less affected limbs. The Nordic walking (NW) training may contribute to reducing the asymmetry in upper and lower limb movements in people with PD. We compared the effects of 11 weeks of NW aerobic training on asymmetrical variables of gait in subjects with mild PD. Fourteen subjects with idiopathic PD, age: 66.8 ± 9.6 years, and Hoehn and Yard stage of 1.5 points were enrolled. The kinematic analysis was performed pre and post-intervention. Data were collected at two randomized walking speeds (0.28 m-s-1 and 0.83 m-s-1) during five minutes on the treadmill without poles. The more affected and less affected body side symmetries (threshold at 5% between sides) of angular kinematics and spatiotemporal gait parameters were calculated. We used Generalized Estimating Equations with Bonferroni post hoc (α = 0.05). Maximal flexion of the knee (p = 0.007) and maximal abduction of the hip (p = 0.041) were asymmetrical pre and became symmetrical post NW intervention. The differences occurred in the knee was less affected and the hip was more affected. We concluded that 11 weeks of NW training promoted similarities in gait parameters and improved knee and hip angular parameters for PD subjects
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