1,721,018 research outputs found
Is modulating virus virulence by induced systemic resistance realistic?
No full textInduction of plant resistance, either achieved by chemicals (systemic acquired resistance, SAR) or by rhizobacteria (induced systemic resistance, ISR) is a possible and/or complementary alternative to manage virus infections in crops. SAR mechanisms operating against viruses are diverse, depending on the pathosystem, and may inhibit virus replication as well as cell-to-cell and long-distance movement. Inhibition is often mediated by salicylic acid with the involvement of alternative oxidase and reactive oxygen species. However, salicylate may also stimulate a separate downstream pathway, leading to the induction of an additional mechanism, based on RNA-dependent RNA polymerase 1-mediated RNA silencing. Thus, SAR and RNA silencing would closely cooperate in the defence against virus infection. Despite tremendous recent progress in the knowledge of SAR mechanisms, only a few compounds, including benzothiadiazole and chitosan have been shown to reduce the severity of systemic virus disease in controlled environment and, more modestly, in open field. Finally, ISR induction, has proved to be a promising strategy to control virus disease, particularly by seed bacterization with a mixture of plant growth-promoting rhizobacteria. However, the use of any of these treatments should be integrated with cultivation practices that reduce vector pressure by the use of insecticides, or by Bt crops
Systemic acquired resistance (50 years after discovery): Moving from the lab to the field
No full textInduction of plant defense(s) against pathogen challenge(s) has been the object of progressively more intense research in the past two decades. Insights on mechanisms of systemic acquired resistance (SAR) and similar, alternative processes, as well as on problems encountered on moving to their practical application in open field, have been carefully pursued and, as far as possible, defined. In reviewing the number of research works published in metabolomic, genetic, biochemical, and crop protection correlated disciplines, the following outline has been adopted: 1, introduction to the processes currently considered as models of the innate immunity; 2, primary signals, such as salicylic acid (SA), jasmonic acid (JA), and abscisic acid (ABA), involved with different roles in the above-mentioned processes; 3, long-distance signals, identified from petiole exudates as mobile signaling metabolites during expressed resistance; 4, exogenous inducers, including the most significant chemicals known to stimulate the plant resistance induction and originated from both synthetic and natural sources; 5, fungicides shown to act as stimulators of SAR in addition to their biocidal action; 6, elusive mechanism of priming, reporting on the most recent working hypotheses on the pretranscriptional ways through which treated plants may express resistance upon pathogen attack and how this resistance can be transmitted to the next generation; 7, fitness costs and benefits of SAR so far reported from field application of induced resistance; 8, factors affecting efficacy of induced resistance in the open field, indicating that forces, unrevealed under controlled conditions, may be operative in the field; 9, concluding remarks address the efforts required to apply the strategy of crop resistance induction according to the rules of integrated pest management
Comparative antifungal effect and mode of action of tetraconazole on Ustilago maydis
No full textTetraconazole, a new, recently introduced antifungal triazole, has been assayed in parallel with a number of standard analogues on various sensitive strains of Ustilago maydis. The values of EC50 and EC90 tetraconazole concentrations, determined on strain ATCC 14826 in agar, were 0.5 × 10-6 and 3.5 × 10-5 m, respectively, in reasonable agreement with those needed to inhibit by 50% and 90%, respectively, the ergosterol biosynthesis in broth cultures. Squalene and 12 sterols have been extracted from the latter, characterized and quantified. Accumulation of 14α-methylsterols and reduction of ergosterol and other late precursors are consistent with the inhibition of 14α-demethylase caused by the title compound
Characterization of field-isolates and derived DMI-resistant strains of Cercospora beticola
No full textCercospora beticola strains with laboratory induced resistance to tetraconazole were compared with their parental WT sensitive strains to evaluate the effects of resistance on fitness and assess whether any change in the sterol biosynthetic pathway was associated to the reduced fungicide sensitivity. In vitro growth rate on agar media and pathogenicity were found to be negatively affected by resistance. The main functional sterols in C. beticola WT strains under investigation were ergosterol, brassicasterol and ergosta-7,22-dienol. Resistant strains showed the same qualitative sterol composition, ruling it out as, per se, a cause for resistance. On the basis of the sterols detected both in sensitive and resistant strains, a possible biosynthetic pathway to the three functional sterols is proposed. Tetraconazole treatment caused, in all sensitive strains, the immediate accumulation of 14α-methylated sterols, which, for inhibitor concentrations up to EC 50 values, were, in order of abundance, 14α-methylergosta-8, 24(28)-dien-3β,6α-diol, eburicol and obtusifoliol. However the data do not support a critical role of the 14-methyl-3,6-diol derivative in the growth arrest of C. beticola. As main difference between sensitive and resistant strains, the formers were found to accumulate higher amounts of 14α-methylated sterols. Although the data do not allow to establish a specific mechanism for resistance, some molecular mechanisms such as target site alterations and sterol biosynthetic pathway can be ruled out as a possible cause for reduced sensitivity
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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