5,636 research outputs found
Anti-inflammatory and antiosteo-clastogenesis properties of endogenous melanocortin receptor type 3 in experimental arthritis
No full textThe development of biological therapies has improved management of rheumatoid arthritis. However, costs and unresponsiveness to therapy in a sizeable proportion of patients limit their use, making it imperative to identify new targets for drug development programs. Here we investigated the melanocortin-receptor type 3 (MC3) pathway. Gene-deficient mice were subjected to a model of serum-transfer-induced arthritis and joints analyzed for gene expression (cytokines, MCs) and morphology. Pharmacological analyses were also conducted in this model. Osteoclastogenesis was studied from bone marrow cells. Mc3−/− mice displayed an exacerbated inflammatory arthritis, associated with prominent bone erosion and higher articular expression of Rankl. Osteoclastogenesis studied from Mc3−/− bone marrow cells revealed a higher degree of responsiveness to Rankl, linked to prolonged NF-κB activation compared to wild types. Up-regulation of a discrete set of inflammatory genes, including Il-1β, Il-6, and Nos2, was measured in Mc3−/− mice, and a marked up-regulation of joint Mc3 accompanied arthritis resolution in wild-type mice. Administration of an MC3 agonist, D[Trp8]-γ-MSH, attenuated disease incidence and severity in wild-type but not Mc3−/− mice. Overall, these findings identify MC3-mediated signaling as a beneficial pathway in experimental arthritis; hence this receptor is a novel target for the development of therapeutics for arthritis.—Patel, H. B., Bombardieri, M., Sampaio, A. L. F., D'Acquisto, F., Gray, M., Grieco, P., Getting, S. J., Pitzalis, C., Perretti, M. Anti-inflammatory and antiosteoclastogenesis properties of endogenous melanocortin receptor type 3 in experimental arthritis
FIGURE 1. Sloanea filiformis. A in Three new species of Sloanea (Elaeocarpaceae)
No full textFIGURE 1. Sloanea filiformis. A) Flowering branch; B) Open flower; C) Detail of stamen; D) Detail of the flower; E) Detail of ovary (Queiroz et al. 2105); F) Detail of the branch with fruits (Amorim et al. 574).Published as part of Sampaio, Daniela & Souza, Vinicius C., 2011, Three new species of Sloanea (Elaeocarpaceae), pp. 45-51 in Phytotaxa 16 on page 46, DOI: 10.11646/phytotaxa.16.1.3, http://zenodo.org/record/481457
FIGURE 3. Sloanea uniflora. A in Three new species of Sloanea (Elaeocarpaceae)
No full textFIGURE 3. Sloanea uniflora. A) Flowering branch (Souza et al. 14379); B) Branch with mature fruits (Santos & Rodrigues 918); C) Detail of ovary; D) Detail of the trichome that covers the ovary; E and F) Detail of stamen (Souza et al. 14379).Published as part of Sampaio, Daniela & Souza, Vinicius C., 2011, Three new species of Sloanea (Elaeocarpaceae), pp. 45-51 in Phytotaxa 16 on page 50, DOI: 10.11646/phytotaxa.16.1.3, http://zenodo.org/record/481457
Eugenia (Myrtaceae) from Mexico: checklist, distribution, and conservation assessments
No full textUc Gala, Victor C., Valdemarin, Karinne Sampaio, Lucas, Eve, Negrão, Raquel, Mazine, Fiorella F. (2023): Eugenia (Myrtaceae) from Mexico: checklist, distribution, and conservation assessments. Phytotaxa 583 (2): 99-140, DOI: 10.11646/phytotaxa.583.2.1, URL: http://dx.doi.org/10.11646/phytotaxa.583.2.
Francisco Antônio de Sampaio: de cirurgião a homem de ciências (Vila de Cachoeira, Bahia, c. 1780)
No full textThis paper discusses Francisco Antonio de Sampaio’s scientific and medical practices. He was a surgeon who worked as a doctor and a naturalist at Vila de Cachoeira, in Bahia. He was charged by Protomedicato with illegal practice of medicine. The article analyzes his correspondence with the Academy of Sciences in Lisbon as well as the manuscripts he sent to that institution between 1783 and 1793. Sampaio’s scientific endeavor aimed to place him as a qualified author of the Republic of Sciences in Portugal and Brazil.Keywords: natural history, Francisco Antonio de Sampaio, history of medicine, history of surgery.O presente artigo discute as práticas médicas e científicas de Francisco Antônio de Sampaio, cirurgião que atuava como médico e naturalista na Vila de Cachoeira, Bahia. Sampaio foi alvo de fiscalização do Protomedicato por extrapolar seu ofício de cirurgião. O artigo analisa a correspondência que Sampaio estabeleceu com a Academia das Ciências de Lisboa, entre 1783 e 1793, bem como os trabalhos manuscritos que enviou à instituição. Sua produção científica visava a sua afirmação como produtor de conhecimento e a sua inserção na República das Ciências lusa e brasileira.Palavras-chave: história natural, Francisco Antonio de Sampaio, história da medicina, história da cirurgia
Optimal activity and thermostability of xylose reductase from Debaryomyces hansenii UFV-170
No full textTo identify the most suitable operating conditions for xylose reductase (XR) activity of Debaryomyces hansenii UFV-170, a 2^2 central composite design face-centered (CCF) was used in this work, varying pH and temperature in the ranges 4–8 and 25–55°C, respectively. The results collected for two response variables, specifically the volumetric and the specific activities, were worked out by Response Surface Methodology. The XR stability was also investigated after storage at different temperatures
FIGURE 2 in Eugenia (Myrtaceae) from Mexico: checklist, distribution, and conservation assessments
No full textFIGURE 2. Map of the biomes of Mexico.Published as part of Uc Gala, Victor C., Valdemarin, Karinne Sampaio, Lucas, Eve, Negrão, Raquel & Mazine, Fiorella F., 2023, Eugenia (Myrtaceae) from Mexico: checklist, distribution, and conservation assessments, pp. 99-140 in Phytotaxa 583 (2) on page 102, DOI: 10.11646/phytotaxa.583.2.1, http://zenodo.org/record/760925
A diversity of resistance sources to Fusarium oxysporum f. sp. pisi found within grass pea germplasm
No full textPurpose: Grass pea is a legume species with recognized resistance to several diseases and thus important for the improvement of related major legume crops. It is infected by the soil-borne fungus Fusarium oxysporum f. sp. pisi (Fop), known as causal agent of pea fusarium wilt. We aim to identify, among grass pea, new sources of resistance against Fop and characterize the detected resistance mechanisms. Methods: A grass pea worldwide collection of accessions was characterized under controlled conditions for response to Fop race 2. Fungal colonization dynamics and potential resistance mechanisms were studied through confocal laser scanning microscopy (CLSM) using Fop race 2 expressing green fluorescent protein (GFP). Results: A quantitative nature of resistance to Fop, ranging from highly to partially resistant and susceptible accessions was detected, with resistance being the most frequent phenotype. Diverse colonization patterns were observed, suggesting the existence of different resistance mechanisms. In the highly resistant accessions, absence of fungal colonization in the vascular tissue was detected, while fungal progression was arrested at the level of roots both in highly resistant and partially resistant accessions. Conclusions: The resistant accessions identified here can be exploited in grass pea breeding for fusarium wilt resistance, and due to the phylogenetic relatedness to pea, potentially contribute to pea improvement
Influence of inhibitory compounds and minor sugars on xylitol production by Debaryomyces hansenii
No full textTo obtain in-depth information on the overall metabolic behavior of the new good xylitol producer Debaryomyces hansenii UFV-170, batch bioconversions were carried out using semisynthetic media with compositions simulating those of typical acidic hemicellulose hydrolysates of sugarcane
bagasse
Bioconversion of D-xylose to xylitol by Debaryomyces hansenii UFV-170: Product formation versus growth
No full textThe relationship between D-xylose-to-xylitol conversion and cell growth was investigated in the yeast Debaryomyces hansenii UFV-170
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