338 research outputs found
Identification of the HetR Recognition Sequence Upstream of hetZ in Anabaena sp Strain PCC 7120
No full textHetR is the master regulator of heterocyst differentiation in Anabaena sp. strain PCC 7120 and has been found to specifically bind to an inverted-repeat-containing region upstream of hetP, a heterocyst differentiation gene. However, no such inverted-repeat sequence can be found in promoters of other genes in the genome. hetZ is a gene involved in early heterocyst differentiation. As shown with the gfp reporter gene, transcription from P-hetZ was correlated to the expression level of hetR and inhibition by RGSGR, the pentapeptide derived from the C terminus of PatS. As detected by electrophoretic mobility shift assay, a recombinant HetR showed specific binding to the region upstream of hetZ, and the binding was inhibited by RGSGR. Tests of a series of the upstream fragments delimited the HetR-binding site to a 40-bp region that shows similarity to that upstream of hetP. The introduction of substitutions of bases conserved in the two HetR-binding sites showed that at least 12 bases are required for recognition by HetR. Deletion of a 51-bp region containing the HetR-binding site completely eliminated the transcription activity of P-hetZ. Based on the HetR recognition sequence of hetZ, those upstream of hetR and patA are proposed.HetR is the master regulator of heterocyst differentiation in Anabaena sp. strain PCC 7120 and has been found to specifically bind to an inverted-repeat-containing region upstream of hetP, a heterocyst differentiation gene. However, no such inverted-repeat sequence can be found in promoters of other genes in the genome. hetZ is a gene involved in early heterocyst differentiation. As shown with the gfp reporter gene, transcription from P-hetZ was correlated to the expression level of hetR and inhibition by RGSGR, the pentapeptide derived from the C terminus of PatS. As detected by electrophoretic mobility shift assay, a recombinant HetR showed specific binding to the region upstream of hetZ, and the binding was inhibited by RGSGR. Tests of a series of the upstream fragments delimited the HetR-binding site to a 40-bp region that shows similarity to that upstream of hetP. The introduction of substitutions of bases conserved in the two HetR-binding sites showed that at least 12 bases are required for recognition by HetR. Deletion of a 51-bp region containing the HetR-binding site completely eliminated the transcription activity of P-hetZ. Based on the HetR recognition sequence of hetZ, those upstream of hetR and patA are proposed
Hetzleine, Jacob F. (Birth, 1883-01-01)
No full textAddress: 42 Court St.65/Pg. 204/1883/M W/Ger./Ger./C./J.M.French, M.D.Original record filed in drawer labeled 'HETZ-HILL, F'
Hilbert, Arthur C. (Birth, 1904-04-08)
No full textAddress: 215 Odeon1645/Pg 117/1904/MW/Ind./Ind./Dr. C. H. MeyersOriginal record filed in drawer labeled 'HETZ-HILL, F'
Hettrich, Ada Ruth C. (Birth, 1893-03-04)
No full textAddress: 397 Elm1946/Pg. 52/1893/F. W./Cincinnati/Cincinnati/Dr. C. G. E. SpeidOriginal record filed in drawer labeled 'HERMAN-HETZ'
Hess, Anna C. (Death, 1876-05-25)
No full textAddress: Longworth St.Age at death: 2 yrsPg 144/1876/449/F W S/City/Dr. C. Betcher/J. Schreiber/Walnut HillsOriginal record filed in drawer labeled 'HERMAN-HETZ'
Hessler, Ella C. (Death, 1888-06-07)
No full textAddress: 426 Colerain Ave.Age at death: 2 yrsPg 63/1888/167/F W S/City/Dr. C. E. Ravogki/Jos. Schreiber/Spring GroveOriginal record filed in drawer labeled 'HERMAN-HETZ'
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