240 research outputs found
Identifying proteins that preferentially bind ATP- over ADP-F-actin
No full textThe cytoskeleton is a dynamic network of proteins that grants cells internal organisation, structure and mobility. This gives cells the ability to participate in all manner of physiological processes, such as immune response, growth and development. However, the same genes and proteins that control these processes can also be altered and participate in pathological events such as cancer and metastasis. The actin cytoskeleton is responsible for the formation of cellular protrusions that allow cells to migrate. The process that gives rise to these protrusions is the polymerisation of actin into its filamentous form, Factin. This is a highly dynamic process that involves regulation by many other proteins and some degree of self-regulation. Actin monomers incorporated into the polarised filament are ATP bound. Upon polymerisation, actin intrinsic hydrolytic activity gives rise to differing states of nucleotide bound conformations throughout the actin filament. Certain proteins are known to bind the aged, ADP-bound portion of F-actin, and contribute to negative regulation for example by promoting depolymerisation. Similarly, we hypothesise that some proteins would preferentially bind the ATP-bound portion of F-actin and be involved in positive regulation of polymerisation.
To investigate this, we optimised a methodology for creating F-actin filaments that mimic the ATP-bound portion using non-hydrolysable analogues of ATP, and constructed affinity chromatography columns that were used to isolate specific interactors. Subsequent mass spectrometry revealed a pool of proteins that preferentially bind ATP-bound over ADP-bound F-actin. One of the identified hits, Eps8, was employed to explore the mechanism of ATP-F-actin binding. Combining molecular biology cloning techniques and super resolution microscopy, we identified Eps8’s SH3 domain as the main regulator of this interaction. Many of the proteins identified as preferentially binding ATP-F-actin contain SH3 domains, suggesting a more generalised role of this domain in the binding to ATP-actin, either directly or indirectly. SH3 domains are known to interact with proline rich sequences. Recent work from our lab has shown the importance of the polyproline domains of members of the WAVE regulatory complex on the formation of actin protrusions. Based on these combined data, we hypothesise that the SH3/polyproline domain interaction could constitute an axis for the recruitment of different proteins involved in actin polymerisation to the site of ATP-F-actin, generating a positive feedback loop of polymerisation. More work, for example using purified proteins and binding assays, is needed to determine the exact method of ATP-F-actin binding, the role it has in positive regulation of polymerisation and its biological relevance
Simphonia, string quartet, F
No full textAn unpublished work. --- An account of this composition, attributed to Pergolesi by Humphrey Claydon is published in Music & letters, Oct., 1938
Evidence on aggregating behaviour of study species.
No full texta<p>Sadovy de Mitcheson et al. (2008);</p>b<p>Claydon (2004);</p>c<p>Froese and Pauly (2003);</p>d<p>Robinson et al. (2008);</p>e<p>Rhodes and Tupper (2008);</p>f<p>Grandcourt et al. (2006).</p>g<p>Ebisawa (2006);</p>h<p>Nanami and Yamada (2009).</p
Women in clinical autonomic research and the autonomic societies: how far have we come in thirty years?
No full textn.a
To Go With The Comet
No full textBritish Art Show 7: In the Days of the Comet
A mixed-media exhibition with work by 39 British artists looking at the idea of the comet as a harbinger of change
Widely recognised as the most ambitious and influential exhibition of contemporary British art, the British Art Show is organised by the Hayward Gallery and has taken place every five years since 1979.
Through paintings, sculpture, installations, video, film and performance, British Art Show 7 explored the ways in which artists conjure histories – distant or near, longingly imagined or all too real – to illuminate our present moment.
Subtitled ‘In the Days of the Comet’, British Art Show 7 took as its motif the idea of the comet as a harbinger of change, a measure of time and a marker of historical recurrence. Besides reflecting these and other cosmological concepts, the works proposed alternative ways of thinking about the ‘here and now’. Many of the 39 artists and artists’ groups made works especially for the exhibition.
Touring to four cities including London, where it was shown at the Hayward, British Art Show 7 featured a number of premieres, including new films by Elizabeth Price, Christian Marclay, Luke Fowler and Nathaniel Mellors. The Hayward showing featured newly produced works by Steve Claydon, Charles Avery and Tris Vonna-Michell.
Curated by Lisa Le Feuvre and Tom Morton, British Art Show 7 opened in Nottingham and, following its London showing at the Hayward Gallery, travelled to Glasgow and Plymouth.
Featured artists
Charles Avery, Karla Black, Becky Beasley, Juliette Blightman, Duncan Campbell, Varda Caivano, Spartacus Chetwynd, Steven Claydon, Cullinan Richards, Matthew Darbyshire, Milena Dragicevic, Luke Fowler, Michael Fullerton, Alasdair Gray, Brian Griffiths, Roger Hiorns, Ian Kiaer, Anja Kirschner & David Panos, Sarah Lucas, Christian Marclay, Simon Martin, Nathaniel Mellors, Haroon Mirza, David Noonan, The Otolith Group, Mick Peter, Gail Pickering, Olivia Plender, Elizabeth Price, Karin Ruggaber, Edgar Schmitz, Maaike Schoorel, George Shaw, Wolfgang Tillmans, Sue Tompkins, Phoebe Unwin, Tris Vonna-Michell, Emily Wardill and Keith Wilson
THEORETICAL INTERPRETATION OF THE ELECTRON SCATTRING SPECTRUM OF METHANE
No full text T. E. Sharp and J. T. Dowell, J. Chem. Phys. 46, 1530 (1967). F. H. Dorman, J. Chem. Phys. 44, 3856 (1966). L. G. Christophorou, M. N. Pisanias, and J. G. Carter, Bulletin Am. Phys. Soc. 16, 1347 (1971).""Author Institution: Battelle Memorial InstitutePotential energy surfaces have been calculated for the lowest resonant states of . These calculations are used to correlate the dissociative electron attachment (DA) and the threshold electron excitation spectrum (TEES) of The peak at 2.5 eV in the TEES spectrum is assigned to the compound state which is bound with respect to the lowest dissociation limits: and . Thus, this resonance state can decay only by autoionization. This assignment is consistent with no being formed by dissociative electron attachment to (the lowest excited states of are not energetically accessible). The peak at 9.0 eV and the peak at 10.4 eV of the DA are assigned to the compound state. It is proposed that the inverse isotope for H production and the large direct isotope effect for production may be the result of the Jahn-Teller splitting of the triple degeneracy of the resonance state
Disability and Identity in Film: Finding the Voices of the Disabled in Cinema
No full textAll art forms take some kind of control over the subject matter they represent and identities can be written into and not just onto the text depending upon the authorial subjectivity and the extent to which any subject may be objectified. Consequently, what is represented typically has little control over how it is represented beyond the textmakers’ methods of description and critique even if that description is grounded in some form of ontological research into that subject. This experience of subjects within a text is something we encounter regularly and it is usually tinged with the ideology of the textmaker, their own identity, and the motives they may have in creating the representation. The autobiography would, on the surface, appear to be an exception to the rule as the author recounts their own subjectivity but I would argue that even this form is not without its objectification of character because the autobiography, even those which purport to be ‘warts and all’, is fundamentally an egoistic text presenting the author and hero or heroine of their own drama. In addition to this, we have also have to recognise the role of ghost writers in constructing the ‘official’ inner world of many so-called autobiographical texts.
[Taken from the first paragraph of the paper
The Epping & Ongar Farmers\u27 Coursing Club Sixth Annual Dinner at the Budworth Hall, Ongar on Wednesday, 9th. April, 1930
No full textClub President Richard Waltham, Vice Presidents Sir D. Cunliffe Smith, James Furze, F. B. Debenham, I ,. Squire, W. Corbett Mighell. Chairman of the Club F. J. Hunt, F. H. Worley, Hon. Sec.
Toast to the King proposed by the Chairman. Toast to the Epping and Ongars Farmers\u27 Coursing Club proposed by Major F. C. Watson. Followed by Presentation of the Cups by the Chairman.
Entertainment provided by Miss Gladys Holliday (on piano), Mdlle Suzette (In Magic and Humour), Mr. Walter Newman (Entertainer), Mr. Arthur Askey (Humourous), Miss Gladys Holliday and Mr. Walter Newman (In Humour and Nonsens), Mr. Harry Claydon (Banjo Solo), Miss Nora Bancroft (Contralto) and Miss Diana Maxwell (Entertainer), also in duets. Accompanist Miss Gladys Holliday, Musical Director Mr. W.J. Chalkley.https://arrow.tudublin.ie/menus20c/1003/thumbnail.jp
Synthesis of Graphene Oxide-Polyacrylic Acid Coated Reverse Osmosis Membranes
No full textReverse osmosis (RO) treatment is a practical option for alleviating potable water scarcity. RO is efficient compared to other technologies, and infrastructure is already in place with the opportunity to modify membranes with few complications. One issue plaguing RO is fouling: microbial and mineral, but antifoulants can reduce both types. The respective antifoulants and processes interact, which necessitates further research to understand the processes in relation to each other and to increase RO efficiency. Two syntheses were investigated to attach graphene oxide (GO) to the membrane. Synthesis One used amination with EDC and NHS, and Synthesis Two used polydopamine (PDA). Synthesis One failed to attach GO to the membrane concluded primarily from FTIR spectroscopy. Synthesis Two was successful based on FTIR and Raman spectroscopy and permeability testing. After Synthesis Two's completion, polyacrylic acid (PAA) was attached to GO through UV light-induced polymerization. Permeability results indicated that the PDA-GO-PAA procedure was a promising synthesis.Civil and Environmental Engineering, Department ofHonors Colleg
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