1,720,954 research outputs found
An Investigation of Informativeness of new microsatellite loci in Camel
No full textZiel der vorliegenden Untersuchungen war es, neue Primer für die Darstellung polymorpher Mikrosatellitenloci in verschiedenen Kamel-Spezies einzusetzen und neue Mikrosatellitenloci auf Informativität zu prüfen.
Einige polymorphe Loci sollten beispielhaft für die Untersuchung der genetischen Unterscheidbarkeit verschiedener Dromedarrassen Kenias und einer Trampeltierrasse Sibiriens verwendet werden.
Für die Untersuchungen stand Probenmaterial von zwei Spezies der Altwelt-Kameliden (Camelus bactrianus und Camelus dromedarius) und zwei Spezies der Neuwelt-Kameliden (Llama pacos und Llama glama) zu Verfügung.
Als DNA-Quelle wurde Vollblut oder aus dem Blut gewonnene Leukozytenfraktion verwendet.
Die Darstellung der Mikrosatellitenloci erfolgte mit Hilfe der PCR und nachfolgender Fragmentlängenanalyse im DNA-Sequenzierautomaten.
Die Zahl der Allele, ihre Fragmentlängen sowie die Allel- und Genotypfrequenzen wurden pro Locus berechnet und als Ausgangsdaten für Informativitätsberechnungen genommen.
Als Parameter der Informativität wurden Heterozygotiegrade, PIC-Index, effektive Anzahl der Allele und Ausschlusswahrscheinlichkeit für einen Markerlocus bzw. für mehrere Markerloci bei der Abstammungskontrolle berücksichtigt.
Es wurden Primerpaare für 34 neu beim Trampeltier sequenzierte Loci getestet.
25 dieser Loci zeigten bei Trampeltier und Dromedar spezifische PCR-Amplifikationen und wurden deswegen in die weiteren Untersuchungen der Informativität einbezogen.
Etwa ein Drittel der Loci erwies sich als monomorph, die übrigen Loci waren polymorph.
Von den 17 polymorphen Loci wiesen neun mehr als zehn Allele auf, ermittelt über alle untersuchten Spezies.
Elf Loci zeigten bei Trampeltier und Dromedar Heterozygotiegrade > 0,5.
Aus den Ausschlusswahrscheinlichkeiten bei der Abstammungskontrolle ließ sich erkennen, dass ein aussagefähiges Markersystem für Elternschaftskontrollen beim Kamel etabliert werden konnte.The goal of the investigations was it to use new primers for the representation of polymorphic microsatellite loci in different camel species and to examine new microsatellite loci for Informativity.
Some polymorphic loci should be used for the investigation of the genetic differentiation of different dromedary races from Kenya and a bactrian race from Sibiria.
For the investigations samples from two species of the old world camels (Camelus bactrianus and Camelus dromedarius) and two species of the new world camels (Llama pacos and Llama glama) were available.
As DNA source full blood or leukocyte was used.
The representation of the microsatellite loci was carried out with the help of the PCR and following fragment length analysis by means of an automated DNA-Sequencer (A.L.F.).
The number of the alleles, their fragment lengths as well as the frequencies of allels and genotypes were computed per locus and taken as original data for estimation of invormativeness.
Primer pairs for 34 sequenced loci were tested.
25 of this loci showed specific PCR amplifications with bactrian and dromedary and were therefore included into the further investigations.
For instance one third of the loci were found as monomorph, the remaining loci were polymorph.
9 from 17 polymorphic loci showed more than ten alleles in all examined species.
The genetic distances between the examined species, computed by means of new microsatellite loci, refer to clear differences between old and new world camels.
With the investigations of some dromedary races from Kenya it was shown that the genetic distances estimated on the basis the new marker loci correlated with geographical distances between the races.
The investigations supplied thus new informative markers for different genetic analysis in camel.
The markers could be also important for the camel breeding (e.g. parentage testing)
An Investigation of Informativeness of new microsatellite loci in Camel
Full text linkThe goal of the investigations was it to use new primers for the representation of polymorphic microsatellite loci in different camel species and to examine new microsatellite loci for Informativity.
Some polymorphic loci should be used for the investigation of the genetic differentiation of different dromedary races from Kenya and a bactrian race from Sibiria.
For the investigations samples from two species of the old world camels (Camelus bactrianus and Camelus dromedarius) and two species of the new world camels (Llama pacos and Llama glama) were available.
As DNA source full blood or leukocyte was used.
The representation of the microsatellite loci was carried out with the help of the PCR and following fragment length analysis by means of an automated DNA-Sequencer (A.L.F.).
The number of the alleles, their fragment lengths as well as the frequencies of allels and genotypes were computed per locus and taken as original data for estimation of invormativeness.
Primer pairs for 34 sequenced loci were tested.
25 of this loci showed specific PCR amplifications with bactrian and dromedary and were therefore included into the further investigations.
For instance one third of the loci were found as monomorph, the remaining loci were polymorph.
9 from 17 polymorphic loci showed more than ten alleles in all examined species.
The genetic distances between the examined species, computed by means of new microsatellite loci, refer to clear differences between old and new world camels.
With the investigations of some dromedary races from Kenya it was shown that the genetic distances estimated on the basis the new marker loci correlated with geographical distances between the races.
The investigations supplied thus new informative markers for different genetic analysis in camel.
The markers could be also important for the camel breeding (e.g. parentage testing).Ziel der vorliegenden Untersuchungen war es, neue Primer für die Darstellung polymorpher Mikrosatellitenloci in verschiedenen Kamel-Spezies einzusetzen und neue Mikrosatellitenloci auf Informativität zu prüfen.
Einige polymorphe Loci sollten beispielhaft für die Untersuchung der genetischen Unterscheidbarkeit verschiedener Dromedarrassen Kenias und einer Trampeltierrasse Sibiriens verwendet werden.
Für die Untersuchungen stand Probenmaterial von zwei Spezies der Altwelt-Kameliden (Camelus bactrianus und Camelus dromedarius) und zwei Spezies der Neuwelt-Kameliden (Llama pacos und Llama glama) zu Verfügung.
Als DNA-Quelle wurde Vollblut oder aus dem Blut gewonnene Leukozytenfraktion verwendet.
Die Darstellung der Mikrosatellitenloci erfolgte mit Hilfe der PCR und nachfolgender Fragmentlängenanalyse im DNA-Sequenzierautomaten.
Die Zahl der Allele, ihre Fragmentlängen sowie die Allel- und Genotypfrequenzen wurden pro Locus berechnet und als Ausgangsdaten für Informativitätsberechnungen genommen.
Als Parameter der Informativität wurden Heterozygotiegrade, PIC-Index, effektive Anzahl der Allele und Ausschlusswahrscheinlichkeit für einen Markerlocus bzw. für mehrere Markerloci bei der Abstammungskontrolle berücksichtigt.
Es wurden Primerpaare für 34 neu beim Trampeltier sequenzierte Loci getestet.
25 dieser Loci zeigten bei Trampeltier und Dromedar spezifische PCR-Amplifikationen und wurden deswegen in die weiteren Untersuchungen der Informativität einbezogen.
Etwa ein Drittel der Loci erwies sich als monomorph, die übrigen Loci waren polymorph.
Von den 17 polymorphen Loci wiesen neun mehr als zehn Allele auf, ermittelt über alle untersuchten Spezies.
Elf Loci zeigten bei Trampeltier und Dromedar Heterozygotiegrade > 0,5.
Aus den Ausschlusswahrscheinlichkeiten bei der Abstammungskontrolle ließ sich erkennen, dass ein aussagefähiges Markersystem für Elternschaftskontrollen beim Kamel etabliert werden konnte
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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