14 research outputs found

    Estimation of Energy Released from Crack in Concrete using Acoustic Emission and Comparison with the Numerical Results

    No full text
    Study relating Acoustic Emission (AE) energy to fracture energy has been conducted in the past and a correlation has been reported between the two. The scope of these studies has remained limited to laboratory size specimens with known crack location and the energy release being investigated on a global scale. The objective of this research is to track the local energy release due to cracking in concrete using AE monitoring and understand the relationship between AE energy and crack energy estimated using nonlinear FE model. This will allow to keep the track of energy release due to cracking and use AE energy as a measure for the structural health condition. The study involves challenges with respect to AE source identification, estimating AE energy at source location accounting for attenuation losses and estimating local energy in the numerical model. These issues are discussed in detail in this thesis and the use of AE in crack monitoring is critically examined. In the first part of the thesis, AE source classification methods including signal-based approach and parameter-based approach are reviewed. The classification methods help distinguish the AE activities due to crack opening from ones related to friction. An approach for signal-based AE classification using the AE signal in the frequency domain is proposed. This approach is then compared to existing bivariate and multivariate parameter-based classification methods. In addition to this, a novel partial power-based method for AE source classification is also proposed. The existing parameter-based classification methods are found to have a similarity of less than 50% in case of bivariate methods and a little over 50 % in the case of the multivariate method when compared to the signal-based method. This is because these methods are unable to notice small differences in AE signals. On the other hand, the partial power-based method has a similarity of about 75 % to the signal-based method. In addition to this, the partial power-based method is much faster than the signal-based approach, thus providing a good alternative to the existing AE classification methods. In the second part, attenuation in AE signals is studied. Experiments on sound concrete and cracked concrete have been performed to study the attenuation in concrete media and through a crack, respectively. AE attenuation due to elastic wave propagation is made under the assumption of a Rayleigh wave and the material attenuation factor (α) is estimated to be 2.473 m-1. Crack attenuation factor (C.A.F.) is introduced to determine the energy loss through a crack. Auto Sensor Test (AST) measurements made during the experiment were used to estimate C.A.F. AST measurements are found to be sensitive to the strain changes within the concrete and are thus able to predict the occurrence of the crack in advance. In the last part, a methodology to estimate the local energy release in the numerical model is proposed and then verified using a notched beam as a test model. A rotating crack approach for modelling is adopted with tension behaviour defined using the Hordijk curve. The proposed methodology is applied to the girder model to estimate the energy released locally. The numerical energy trend thus calculated is compared to the AE energy trend at the crack location. The AE energy predicts the occurrence of the first flexure crack at 90% of the cracking load as per numerical energy. A possible explanation for this is that AE can also detect the presence of the microcracks, which the current numerical model cannot. On comparing the estimated energies released due to AE and numerical model in the flexure zone it can be concluded that the relationship between the AE energy and numerical energy is non-linear. Local energy release trend for AE and the numerical model with increasing load is similar when the flexure cracks are generated, although slight deviations start to occur when the shear crack is created

    Assessment of climate induced damage in decorated oak wooden panels

    No full text
    Climate induced damage in decorated oak wooden panels is considered to be a high risk for the preeminent museum collections. To advise museums on the development of future sustainable preservation strategies and to define rational guidelines for indoor climate specifications, climate induced physical and mechanical damage has been analysed in a collection study, experimental testing of mock-ups and by finite element modelling. The collection study consisted of the development of a comprehensive methodology to select objects of interest from the collection and analyse their condition using a combination of visual inspection and archival searches. Mock-up samples of wooden panels with representative structural elements were exposed to varying climate conditions in climate controlled rooms and monitored with state-of-the-art experimental mechanics equipment. Further the collection study and experimental testing were used to inform the development of a finite element model of crack growth under 3-point bending. The work was performed within the Cimate4Wood project, a multidisciplinary collaboration between conservators and scientists work. The paper presents the methodology of the museum study and results from the museum study, experimental testing and modelling.Structural Integrity & Composite

    Floral Transition And Nitric Oxide Emission During Flower Development In Arabidopsis Thaliana Is Affected In Nitrate Reductase-deficient Plants

    No full text
    The nitrate reductase (NR)-defective double mutant of Arabidopsis thaliana (nia1 nia2) has previously been shown to present a low endogenous content of NO in its leaves compared with the wild-type plants. In the present study, we analyzed the effect of NR mutation on floral induction and development of A. thaliana, as NO was recently described as one of the signals involved in the flowering process. The NO fluorescent probes diaminofluorescein-2 diacetate (DAF-2DA) and 1,2-diaminoanthraquinone (1,2-DAA) were used to localize NO production in situ by fluorescence microscopy in the floral structures of A. thaliana during floral development. Data were validated by incubating the intact tissues with DAF-2 and quantifying the DAF-2 triazole by fluorescence spectrometry. The results showed that NO is synthesized in specific cells and tissues in the floral structure and its production increases with floral development until anthesis. In the gynoecium, NO synthesis occurs only in differentiated stigmatic papillae of the floral bud, and, in the stamen, only anthers that are producing pollen grains synthesize NO. Sepals and petals do not show NO production. NR-deficient plants emitted less NO, although they showed the same pattern of NO emission in their floral organs. This mutant blossomed precociously when compared with wild-type plants, as measured by the increased caulinar/rosette leaf number and the decrease in the number of days to bolting and anthesis, and this phenotype seems to result from the markedly reduced NO levels in roots and leaves during vegetative growth. Overall, the results reveal a role for NR in the flowering process. © The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.49711121121Barroso, J.B., Corpas, F.J., Carreras, A., Sandalio, L.M., Valderrama, R., Palma, J.M., Lupiáñez, J.A., del Río, L.A., Localization of nitric-oxide synthase in plant peroxisomes (1999) J. Biol. Chem, 274, pp. 36729-36733Corpas, F.J., Barroso, J.B., Carreras, A., Quirós, M., Leon, A.M., Cellular and subcellular localization of endogenous nitric oxide in young and senescent pea plants (2004) Plant Physiol, 136, pp. 2722-2733Dacres, H., Narayanaswamy, R., Evaluation of 1,2-diaminoanthraquinone (DAA) as a potential reagent system for detection of (2005) Microchim. Acta, 152, pp. 35-45Dean, J.V., Harper, J.E., Nitric oxide and nitrous oxide production by soybean and winged bean during the in vivo nitrate reductase assay (1986) Plant Physiol, 82, pp. 718-723Dembinski, E., Wisniewska, I., RaczynskaBojanowska, K., The efficiency of protein synthesis in maize depends on the light regulation of the activities of the enzymes of nitrogen metabolism (1996) J. Plant Physiol, 149, pp. 466-468Delledonne, M., NO news is good news for plants (2005) Curr. Opin. Plant Biol, 8, pp. 390-396Delledonne, M., Xia, Y.J., Dixon, R.A., Lamb, C., Nitric oxide functions as a signal in plant disease resistance (1998) Nature, 394, pp. 585-588Desikan, R., Cheung, M.K., Bright, J., Henson, D., Hancock, J.T., Neill, S.J., ABA, hydrogen peroxide and nitric oxide signaling in stomata guard cells (2004) J. Exp. Bot, 55, pp. 205-212Green, L.C., Wagner, D.A., Glogowski, J., Skipper, P.L., Wishnock, J.S., Tannenbaum, S.R., Analysis of nitrate, nitrite, and [15N] nitrate in biologicals fluids (1982) Anal. Biochem, 126, pp. 131-138Guo, F.-Q., Okamoto, M., Crawford, N.M., Identification of a plant nitric oxide synthase gene involved in hormonal signaling (2003) Science, 302, pp. 100-103Halbach, O.B., Nitric oxide imaging in living neuronal tissues using fluorescent probes (2003) Nitric Oxide Biol. Chem, 9, pp. 217-228He, Y., Tang, R.H., Hao, Y., Stevens, R.D., Cook, C.W., Nitric oxide represses the Arabidopsis floral transition (2004) Science, 305, pp. 1968-1971Heiduschka, P., Thanos, S., 1(998) NO production during neuronal cell death can be directly assessed by a chemical reaction in vivo Neuroreport, 9, pp. 4051-4057Henderson, I.R., Dean, C., Control of Arabidopsis flowering: The chill before the bloom (2004) Development, 131, pp. 3829-3838Keefer, L.K., Nims, R.W., Davies, K.M., Wink, D.A., NONOates' (1-substituted diazen-1-ium-1,2-diolates) as nitric oxide donors: Convenient nitric oxide dosage forms (1996) Methods Enzymol, 268, pp. 281-293Kojima, H., Nakatsubo, N., Kikuchi, K., Kawahara, S., Kirino, Y., Nagoshi, H., Hirata, Y., Nagano, T., Detection and imaging of nitric oxide with novel fluorescent indicators: Diaminofluoresceins (1998) Anal. Chem, 70, pp. 2446-2453Lakowicz, J.R., Gryczynski, I., Gryczynski, Z., High throughput screening with multiphoton excitation (1999) J. Biomol. Screen, 4, pp. 355-361Lamattina, L., Garcia-Mata, C., Graziano, M., Pagnussat, G., Nitric oxide: The versatility of an extensive signal molecule (2003) Annu. Rev. Plant Biol, 54, pp. 109-136Magalhaes, J.R., Monte, D.C., Durzan, D., Nitric oxide and ethylene emission in Arabidopsis thaliana (2000) Physiol. Mol. Biol. Plants, 6, pp. 117-127McInnis, S.M., Desikan, R., Hancock, J.T., Hiscock, S.J., Production of reactive oxygen species and reactive nitrogen species by angiosperm stigmas and pollen: Potential signaling crosstalk (2006) New Phytol, 172, pp. 221-228Modolo, L.V., Cunha, F.Q., Braga, M.R., Salgado, I., Nitric oxide synthase-mediated phytoalexin accumulation in soybean cotyledons in response to the Diaporthe phaseolorum f. sp. meridionalis (2002) Plant Physiol, 130, pp. 1288-1297Modolo, L.V., Augusto, O., Almeida, I.M., Magalhaes, J.R., Salgado, I., Nitrite as the major source of nitric oxide production by Arabidopsis thaliana in response to Pseudomonas syringae (2005) FEBS Lett, 579, pp. 3814-3820Modolo, L.V., Augusto, O., Almeida, I.M.G., Pinto-Maglio, C.A.F., Oliveira, H.C., Seligman, K., Salgado, I., Decreased arginine and nitrite levels in nitrate reductase-deficient Arabidopsis thaliana plants impair nitric oxide synthesis and the hypersensitive response to Pseudomonas syringae (2006) Plant Sci, 171, pp. 34-40Mouradov, A., Cremer, F., Coupland, G., Control of flowering time: Interacting pathways as a basis for diversity (2002) Plant Cell, 14, pp. S111-S130Murashige, T., Skoog, F., A revised medium for rapid growth and bio assay with tobacco tissue cultures (1962) Physiol. Plant, 15, pp. 473-497Neill, S., Bright, J., Desikan, R., Hancock, J., Harrison, J., Wilson, I., Nitric oxide evolution and perception (2007) J. Exp. Bot, 59, pp. 25-35Neill, S.J., Desikan, R., Clarke, A., Hancock, J.T., Nitric oxide is a novel component of abscisic acid signaling in stomatal guard cells (2002) Plant Physiol, 128, pp. 13-16Pagnussat, G.C., Simontacchi, M., Puntarulo, S., Lamattina, L., Nitric oxide is required for root organogenesis (2002) Plant Physiol, 129, pp. 954-956Pedroso, M.C., Magalhães, J.R., Durzan, D., A nitric oxide burst precedes apoptosis in angiosperm and gymnosperm callus cells and foliar tissues (2000) J. Exp. Bot, 51, pp. 1027-1036Planchet, E., Jagadis Gupta, K., Sonoda, M., Kaiser, W.M., Nitric oxide emission from tobacco leaves and cell suspensions: Rate limiting factors and evidence for the involvement of mitochondrial electron transport (2005) Plant J, 41, pp. 732-743Prado, A.M., Porterfield, D.M., Feijó, J.A., Nitric oxide is involved in growth regulation and re-orientation of pollen tubes (2004) Development, 131, pp. 2707-2714Ribeiro, E.A., Cunha, F.Q., Tamashiro, M.S.C., Martins, I.S., Growth phase-dependent subcellular localization of nitric oxide synthase in maize cells (1999) FEBS Lett, 445, pp. 283-286Rockel, P., Strube, F., Rockel, A., Wildt, J., Kaiser, W.M., Regulation of nitric oxide (NO) production by plant nitrate reductase in vivo and in vitro (2002) J. Exp. Bot, 53, pp. 103-110Salgado, I., Modolo, L.V., Augusto, O., Braga, M.R., Oliveira, H.C., Mitochondrial nitric oxide synthesis during plant-pathogen interactions: Role of nitrate reductase in providing substrates (2006) Nitric oxide in plant growth, development and stress physiology, pp. 239-254. , Edited by Lamattina, L. and Polacco, J.C. pp, Springer-Verlag, BerlinSalgado, I., Saviani, E.E., Modolo, L.V. and Braga, M.R. (2004) Nitric oxide signaling in plant defence responses to pathogen attack. In Advances in Plant Physiology. Edited by Hemantaranjan, A. 7, pp. 117-137. Scientific Publishers, Jodhpur, IndiaSchmidt, H.H.H.W., Watler, U., NO at work (1994) Cell, 78, pp. 919-925Simpson, G.G., NO flowering (2005) BioEssays, 27, pp. 239-241Simpson, G.G., Dean, C., Flowering - Arabidopsis, the rosetta stone of flowering time? (2002) Science, 296, pp. 285-289Smyth, D.R., Bowman, J.L., Meyerowitz, E.M., Early flower development in Arabidopsis (1990) Plant Cell, 2, pp. 755-767Stohr, C., Stremlau, S., Formation and possible roles of nitric oxide in plant roots (2006) J. Exp. Bot, 57, pp. 463-470Stuehr, D.J., Structure-function aspects in the nitric oxide synthases (1997) Annu. Rev. Pharmacol. Toxicol, 37, pp. 339-359Su, W., Huber, S.C., Crawford, N.M., Identification in vitro of a post-translational regulatory site in the hinge 1 region of Arabidopsis nitrate reductase (1996) Plant Cell, 8, pp. 519-527Wilkinson, J.Q., Crawford, N.M., Identification of the arabidopsis chl3 gene as the nitrate reductase structural gene NIA2 (1991) Plant Cell, 3, pp. 461-471Wilkinson, J.Q., Crawford, N.M., Identification and characterization of a chlorate-resistant mutant of Arabidopsis thaliana with mutations in both nitrate reductase structural genes NIA1 and NIA2 (1993) Mol. Gen. Genet, 239, pp. 289-297Yamamoto, A., Katou, S., Yoshioka, H., Doke, N., Kawakita, K., Nitrate reductase, a nitric oxide-producing enzyme: Induction by pathogen signals (2003) J. Gen. Plant Pathol, 69, pp. 218-229Yamamoto-Katou, A., Katou, S., Yoshioka, H., Doke, N., Kawakita, K., Nitrate reductase is responsible for elicitin-induced nitric oxide production in Nicotiana benthamiana (2006) Plant Cell Physiol, 47, pp. 726-735Yamasaki, H., Sakihama, Y., Simultaneous production of nitric oxide and peroxynitrite by plant nitrate reductase: In vitro evidence for the NR-dependent formation of active nitrogen species (2000) FEBS Lett, 468, pp. 89-92Ye, X., Kim, W.S., Rubakhin, S.S., Sweedler, J.V., Measurement of nitric oxide by 4,5-diaminofluorescein without interferences (2004) Analyst, 129, pp. 1200-1205Zemojtel, T., Fröhlich, A., Palmieri, M.C., Kolanczyk, M., Mikula, I., Wyrwicz, L.S., Wanker, E.E., Durner, J., Plant nitric oxide synthase: A never-ending story? (2006) Trends Plant Sci, 11, pp. 524-525Zhang, X., Kim, W.S., Hatcher, N., Potgieter, K., Moroz, L.L., Gillette, R., Sweedler, J.V., Interfering with nitric oxide measurements - 4,5-diaminofluorescein reacts with dehydroascorbic acid and ascorbic acid (2002) J. Biol. Chem, 277, pp. 48472-48478Zhao, M.G., Tian, Q.T., Zhang, W.H., Nitric oxide synthase-dependent nitric oxide production is associated with salt tolerance in Arabidopsis (2007) Plant Physiol, 144, pp. 206-21

    Suppression of Underwater Noise Induced by Cavitation: SONIC

    No full text
    AbstractIn EU FP7 project SONIC, partners set out in October 2012 to study the underwater radiated noise of ships and shipping. The objectives of the project were (1) to study the numerical and experimental techniques to determine the underwater noise; (2) to develop methods for mapping the noise of ships and shipping; and (3) to determine mitigation measures to reduce the underwater radiated noise.Numerical methods focused on determination of the cavitation extent and dynamics on propellers which is the main source of noise of commercial shipping. Research also focused on methods to determine the underwater radiated noise from machinery. Experimental methods in model test facilities have been studied and validated against dedicated full scale measurements. The ship noise source levels obtained from these numerical and experimental methods provide input to shipping noise mapping tools to determine the overall underwater noise in a certain sea area.Based on the experience gained in the SONIC project, a set of guidelines for regulators concerned with underwater radiated noise of ships were developed together with the AQUO project. These guidelines discuss the definitions, numerical and experimental methods and mitigation solutions for underwater radiated noise.This paper gives an overview of the work done by all partners in the SONIC project

    Monitoring programme for the Maasvlakte 2. Part III: The effects of underwater sound

    No full text
    The Port of Rotterdam is expanding to meet the growing demand to accommodate large cargo vessels. The construction of Maasvlakte 2 (MV2) started in September 2008. One of the licensing conditions is the monitoring of the underwater sound produced during its construction, with an emphasis on the establishment of acoustic source levels of the trailing suction hopper dredgers (TSHDs) during their various activities: dredging, transport and discharge of sediment. TNO (Netherlands Organisation for Applied Scientific Research) Sonar and Acoustics carried out measurement and analysis activities for this monitoring. During an initial measurement campaign in September 2008, background measurements were performed in the absence of dredging. Source level and background sound measurements were made in the dredging area while MV2 dredging activities were underway in September 2009. In a final phase of the study, possible effects of underwater sound on marine fauna were considered for scenarios with and without dredgers. In this article, the principal results of the research are described and discussed in the context of the effects predicted in the Environmental Impact Assessment.Hydraulic EngineeringCivil Engineering and Geoscience

    Risks of mining to salmonid-bearing watersheds

    No full text
    Mining provides resources for people but can pose risks to ecosystems that support cultural keystone species. Our synthesis reviews relevant aspects of mining operations, describes the ecology of salmonid-bearing watersheds in northwestern North America, and compiles the impacts of metal and coal extraction on salmonids and their habitat. We conservatively estimate that this region encompasses nearly 4000 past producing mines, with present-day operations ranging from small placer sites to massive open-pit projects that annually mine more than 118 million metric tons of earth. Despite impact assessments that are intended to evaluate risk and inform mitigation, mines continue to harm salmonid-bearing watersheds via pathways such as toxic contaminants, stream channel burial, and flow regime alteration. To better maintain watershed processes that benefit salmonids, we highlight key windows during the mining governance life cycle for science to guide policy by more accurately accounting for stressor complexity, cumulative effects, and future environmental change.This review is based on an October 2019 workshop held at the University of Montana Flathead Lake Biological Station (more information at https://flbs.umt.edu/ newflbs/research/working-groups/mining-and-watersheds/). We thank E. O’Neill and other participants for valuable contributions. A. Beaudreau, M. LaCroix, P. McGrath, K. Schofield, and L. Brown provided helpful reviews of earlier drafts. Three anonymous reviewers provided thoughtful critiques that greatly improved the manuscript. The views expressed in this article are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency. Our analysis comes from a western science perspective and hence does not incorporate Indigenous knowledge systems. We acknowledge this gap and highlight that the lands and waters we explore in this review have been stewarded by Indigenous Peoples for millennia and continue to be so. Funding: The workshop was cooperatively funded by the Wilburforce Foundation and The Salmon Science Network funded by the Gordon and Betty Moore Foundation. Author contributions: C.J.S. led the review process, writing, and editing. C.J.S. and E.K.S. co-organized the workshop. E.K.S. and J.W.M. extensively contributed to all aspects of the review conceptualization, writing, and editing. A.R.W., S.A.N., J.L.E., D.M.C., S.L.O., R.L.M., F.R.H., D.C.W., and J.W. significantly contributed to portions of the review conceptualization, writing, and editing. J.C., M.Ca., M.Co., C.A.F., G.K., E.D.L., R.M., V.M., J.K.M., M.V.M., and N.S. provided writing and editing and are listed alphabetically. Competing interests: The authors declare that they have no competing interests. Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials.Ye

    Advances and new applications using the acousto-optic effect in optical fibers

    No full text
    This work presents a short review of the current research on the acousto-optic mechanism applied to optical fibers. The role of the piezoelectric element and the acousto-optic modulator in the excitation of flexural and longitudinal acoustic modes in the frequency range up to 1.2 MHz is highlighted. A combination of the finite elements and the transfer matrix methods is used to simulate the interaction of the waves with Bragg and long period gratings. Results show a very good agreement with experimental data. Recent applications such as the writing of gratings under the acoustic excitation and a novel viscometer sensor based on the acousto-optic mechanism are discussed. © 2013 The Author(s)

    Evaluación de alternativas de pavimentación para vías de bajos volúmenes de tránsito

    No full text
    Trabajo de investigaciónEn los últimos años, tanto en Colombia como en otros países suramericanos como Ecuador, Argentina, Bolivia y Perú, se han venido realizando estudios e investigaciones sobre métodos y alternativas para rehabilitación y mantenimiento de vías terciarias y de bajos volúmenes de tránsito, que permitan un uso y aprovechamiento de recursos naturales y nuevos materiales a menores costos, de forma que se logre un equilibrio económico, técnico y ambiental; llegando a proponer soluciones que favorezcan el desarrollo de las regiones donde se aplican tales soluciones, en forma sostenible. De estos estudios han surgido diferentes documentos con propuestas y descripciones de tales alternativas, no obstante diferentes circunstancias impiden la divulgación de tal conocimiento. Actualmente estas alternativas se encuentran clasificadas en tres tipos de acuerdo a sus características de desarrollo, objetivos y funciones. Dado que la implementación de estas es limitado en el país, debido al desconocimiento y poca información sobre las mismas; se presenta este documento que hace una descripción de sus aspectos técnicos ambientales y económicos, y un modelo de elección para la aplicación en las vías colombianas, buscando crear una fuente de información básica para el conocimiento y aplicación de dichas alternativas.EspecializaciónEspecialista en Ingeniería de Pavimento

    Лазер-индуцированная флуоресцентная спектроскопия в диагностике тканевой гипоксии (обзор)

    No full text
    The aim of review is to discuss the results of studies on diagnosis of tissue hypoxia by laser-induced spectroscopy, as well as to identify promising trends and prospects of this technique for its further application in experimental and clinical medicine.The review presents the findings of studies of the fluorescence intensity of endogenous fluorophore molecules (reduced nicotinamide adenine dinucleotide, oxidized flavin adenine dinucleotide) as markers of ischemic injury of internal organs (brain, heart, liver, kidneys, etc.). The principles of fluorescence laser-induced spectroscopy in vivo are discussed. The historical aspects of the subject are also covered. The results of the use of the technique in experimental and clinical studies of tissue hypoxia and ischemia are shown. Difficulties in interpreting the intensity values of autofluorescent signal of the studied molecules are revealed. It was noted that the tissue autofluorescence in a long-term anoxia remains unknown, and there are no structured ideas about the impact of exogenous and endogenous factors on autofluorescence intensity.In conclusion, the use of laser-induced fluorescence spectroscopy to diagnose tissue ischemia is a promising area of experimental and clinical medicine, which still has various unresolved issues, despite a large number of studies in this domain.Цель обзора — анализ опыта диагностики тканевой гипоксии методом лазер-индуцированной спектроскопии, а также выявление перспективных направлений и потенциальных возможностей данного метода для дальнейшего его применения в экспериментальной и клинической медицине.В обзоре представили данные исследований интенсивности флуоресценции эндогенных молекул-флуорофоров (никотинадениндинуклеотида восстановленного, флавинадениндинуклеотида окисленного) как маркеров ишемического повреждения внутренних органов (мозга, сердца, печени, почек и др.). Рассмотрели принципы метода флуоресцентной лазер-индуцированной спектроскопии in vivo. Уделили внимание историческим аспектам темы. Показали результаты применения метода в экспериментальных и клинических исследованиях тканевой гипоксии и ишемии. Выявили трудности в интерпретации значений интенсивности аутофлуоресцентного сигнала исследуемых молекул. Отметили, что неизвестным остается период сохранения способности ткани к аутофлуоресценции в условиях длительной аноксии, отсутствуют систематизированные представления о влиянии экзогенных и эндогенных факторов на интенсивность аутофлуоресценции.Сделали вывод, что применение метода лазер-индуцированной флуоресцентной спектроскопии с целью диагностики тканевой ишемии является перспективным направлением в экспериментальной и клинической медицине, не исчерпавшим себя, и оставляющим ряд нерешенных вопросов, несмотря на большое количество исследований в данной области
    corecore