100,901 research outputs found
Data characterizing diurnal rhythms in the number of peripheral CD8α− and CD8α+ γδ T cells in domestic pigs
No full textThis data article is related to the original research article “Diurnal rhythms in peripheral blood immune cell numbers of domestic pigs” of Engert et al. [1] and describes diurnal rhythms in the number of CD8α− and CD8α+ γδ T cells in peripheral blood of domestic pigs. Blood samples were taken from 18 animals over periods of up to 50 h and immune cell subtypes were determined by flow cytometry. Diurnal rhythmicity of cell numbers of γδ T cell subtypes was analyzed with cosinor analysis and different properties of rhythmicity (mesor, amplitude, and peak time) were calculated. In addition, associations between cell numbers of the investigated cell types in porcine blood with plasma cortisol concentration, hematocrit, and experimental conditions were identified with linear mixed model analysis. Keywords: Diurnal rhythm, Immune system, Gamma-delta T cell, CD8 alpha, Sus scrofa domestic
Letter, [Author unclear] to Paulina T. Merritt
No full textHandwritten letter to Paulina Merritt from an unknown author, October 1, 1876.
Engineering of cartilage tissue constructs in a 3-dimensional perfusion bioreactor culture system under controlled oxygen tension
No full textThe most relevant results generated in this thesis can be summarized as follow:
· Adult human articular chondrocytes (AHAC) from elderly individuals expanded in culture
medium supplemented with the growth factors TGFβ-1, FGF-2 and PDGF and subsequently
cultured in 3-d pellets had an enhanced chondrogenic capacity when exposed to more
physiological (i.e. 5%) oxygen levels.
· In correlation with the enhanced tissue forming capacity of AHAC from elderly donors under
low oxygen tension, the mRNA expression levels of selective matrix degrading enzymes
were reduced as compared to conventional in vitro oxygen culture condition.
· We developed an integrated bioreactor system, which streamlines within a single device the
phases of perfusion cell seeding and prolonged perfusion culture of cell seeded scaffolds in
vitro.
· The culturing of uniformly seeded adult human articular chondrocytes under direct perfusion,
where cells are continuously exposed to a normoxic range of oxygen levels, can maintain a
uniform distribution of viable cells throughout thick porous scaffolds as compared to
statically cultured constructs.
· The culturing of constructs uniformly seeded with adult human articular chondrocytes under
a more physiological range of oxygen resulted in a higher chondrogenic differentiation as
compared to culture under normoxic levels. Anyhow, this effect was less pronounced as
compared to statically cultured cell constructs or micromass cell pellets, possibly due to the
flow induced shear forces.
· Reduced perfusion flow rates applied to chondrocytes on porous scaffolds significantly
induced more cartilaginous tissue in the presents of low vs. high oxygen levels. However the
effects of low oxygen were not as marked as in pellet culture
Handwritten biographical information on Paulina T. McClung Merritt
No full textA handwritten biography of Paulina T. McClung Merritt by an unknown author, 1892.
Heterogeneous and tissue-specific regulation of effector T cell responses by IFN-gamma during Plasmodium berghei ANKA infection.
No full textIFN-γ and T cells are both required for the development of experimental cerebral malaria during Plasmodium berghei ANKA infection. Surprisingly, however, the role of IFN-γ in shaping the effector CD4(+) and CD8(+) T cell response during this infection has not been examined in detail. To address this, we have compared the effector T cell responses in wild-type and IFN-γ(-/-) mice during P. berghei ANKA infection. The expansion of splenic CD4(+) and CD8(+) T cells during P. berghei ANKA infection was unaffected by the absence of IFN-γ, but the contraction phase of the T cell response was significantly attenuated. Splenic T cell activation and effector function were essentially normal in IFN-γ(-/-) mice; however, the migration to, and accumulation of, effector CD4(+) and CD8(+) T cells in the lung, liver, and brain was altered in IFN-γ(-/-) mice. Interestingly, activation and accumulation of T cells in various nonlymphoid organs was differently affected by lack of IFN-γ, suggesting that IFN-γ influences T cell effector function to varying levels in different anatomical locations. Importantly, control of splenic T cell numbers during P. berghei ANKA infection depended on active IFN-γ-dependent environmental signals--leading to T cell apoptosis--rather than upon intrinsic alterations in T cell programming. To our knowledge, this is the first study to fully investigate the role of IFN-γ in modulating T cell function during P. berghei ANKA infection and reveals that IFN-γ is required for efficient contraction of the pool of activated T cells
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Pelevin’s Trinity in the novel “t”: author – protagonist – reader
No full textThe article attempts to interpret Pelevin's artistic strategy in the novel "T" by exploring its subject organization and addressing the key problems of the author, the protagonist, and the reader as they are seen by the researcher. The article analyzes the peculiarities of constructing the narrative reality in the novel "T", and goes on to discuss Pelevin's philosophic models of the development of the humankind, and the emergence of his new anthropology
The Euramet Metrology Research Programme Project: Implementing the new kelvin (InK)
No full textCurrently almost all temperature measurement around the world is based on traceability to a defined scale, either the International Temperature Scale of 1990, ITS-90 or, below 1 K, the Provisional Low Temperature Scale of 2000, PLTS-2000. These scales are empirical in basis, reliant upon a set of fixed points whose temperatures were determined a priori by primary thermometry. However recent technical advances in temperature metrology, the advent of the evolving mise en pratique for the definition of the kelvin (MeP-K) and the forthcoming kelvin redefinition in terms of a fixed value of the Boltzmann constant provide a unique opportunity to fundamentally change the practice of temperature measurement.
In the light of this a European Metrology Research Programme project, known as Implementing the new kelvin (InK), was established; the first phase of which was from Oct 2012 to September 2015. The project has 13 partners and 8 collaborators/universities from around the world working in the field of primary thermometry.
The research to be performed in the InK project has two main thrusts:
The development of primary thermometry methods that both challenge and supplant the defined scales at high (>1000 C) and low (<1 K) temperatures. This will enable, for the first time, and at these temperature extremes, to directly realise and disseminate thermodynamic temperature, with uncertainties that are competitive with the defined scales.
Between these temperatures new values of T – T90 (by a variety of methods) with the lowest uncertainties (1 mK) will be determined. This is required for several reasons: a) in the short term to provide ultra-reliable T – T90 data for the MeP-K, b) to develop the primary thermometry techniques needed to progressively supplant defined scales and c) provide the background thermodynamic temperature data required for any successor temperature scale, namely ITS20XX.
This presentation will give an overview of the project, initial results will be presented and its impact into world thermometry will be discussed
Measuring industry-science links through inventor-author relations: A profiling method
No full textIn this pilot study we examine the performance of text-based profiling in recovering a set of validated inventor-author links. In a first step we match patents and publications solely based on their similarity in content. Next, we compare inventor and author names on the highest ranked matches for the occurrence of name matches. Finally, we compare these candidate matches with the names listed in a validated set of inventor-author names. Our text-based profile methodology performs significantly better than a random matching of patents and publications, suggesting that text-based profiling is a valuable complementary tool to the name searches used in previous studies.innovation; industry-science links; text-based profiling;
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