71 research outputs found
The effectiveness of various chelates used alone or in combination with sodium hypochlorite in the removal of calcium hydroxide from root canals
No full textAim: To evaluate the effectiveness of various chelates used alone or in combination with sodium hypochlorite (NaOCl) in the removal of calcium hydroxide (Ca(OH)2
) from root canals.
Materials and Methods: The root canals of 72 mandibular incisors were prepared up to the ProTaper F2 file. Among these, six randomly selected teeth were used as negative and positive controls, while the root canals of the remaining 66 were filled with Ca(OH)2 paste for 1 week. Then, the experimental group specimens were divided into six groups (n = 11). The access cavities were reopened and the Ca(OH)2 paste in each group was removed using the following solutions: 2.5 mL ethylenediaminetetraacetic acid (EDTA; Group 1), 2.5 mL peracetic acid (PAA; Group 2), 2.5 mL QMix (Group 3), 2.5 mL NaOCl/2.5 mL EDTA (Group 4), 2.5 mL NaOCl/2.5 mL PAA (Group 5), and 2.5 mL NaOCl/2.5 mL QMix (Group 6). Digital photographs of longitudinally split specimens were imported into image analyzer software, and the amount of residual Ca (OH) 2 was recorded as a percentage of the overall canal surface area. The results were analyzed using Kruskal–Wallis and Conover–Dunn tests.
Results: The canal walls in the positive control group were completely covered with Ca(OH) 2 compared with those in the negative control group. The lowest Ca(OH)2 removal efficiency was observed for Group 4 (P < 0.001), while Group 6 showed favorable results (P < 0.05).
Conclusions: QMix combined with NaOCl can remove Ca(OH)2
from root canals as effectively as 17% EDTA and 1% PAA. The type and sequence of irrigants are more important than the total irrigant volume for effective Ca(OH) 2 removal
Biofilm-forming ability and adherence to poly-(methyl-methacrylate) acrylic resin materials of oral Candida albicans strains isolated from HIV positive subjects
No full textDolapci, Istar/0000-0002-6443-4612WOS: 000332444800005PubMed: 24605203PURPOSE. This study evaluated the adhesion to acrylic resin specimens and biofilm formation capability of Candida albicans strains isolated from HIV positive subjects' oral rinse solutions. MATERIALS AND METHODS. The material tested was a heat-cured acrylic resin (Acron Duo). Using the adhesion and crystal violet assays, 14 oral Candida albicans isolated from HIV-positive subjects and 2 references Candida strains (C. albicans ATCC 90028 and C. albicans ATCC 90128) were compared for their biofilm production and adhesion properties to acrylic surfaces in vitro. RESULTS. There were no significant differences in adhesion (P=.52) and biofilm formation assays (P=.42) by statistical analysis with Mann-Whitney test. CONCLUSION. Denture stomatitis and increased prevalence of candidal carriage in HIV infected patients is unlikely to be related to the biofilm formation and adhesion abilities of C. albicans to acrylic resin materials
Impact Of Different File Systems On The Amount Of Apically Extruded Debris During Endodontic Retreatment
No full textObjective: The goal of present study was to determine the effect of different nickel–titanium file systems on the amount of apically extruded debris during endodontic retreatment: D-RaCe retreatment systems, EdgeFile XR retreatment rotary files, and Reciproc R40. Materials and Methods: Thirty-six single-rooted prepared mandibular premolar teeth were filled with Gutta-percha and AH Plus sealer. The teeth were then randomly assigned into three groups (n = 12) for retreatment. The endodontic retreatment was performed as follows: D-RaCe, EdgeFile XR, Reciproc 40. Debris extruded apically during the retreatment was collected into preweighed Eppendorf tubes. An incubator was used to store tubes at 70° C for 5 days. The initial weight was subtracted from final weight of the Eppendorf tubes to calculate the weight of the dry extruded debris for each group. The data obtained were evaluated using Welch analysis of variance and Games-Howell post-hoc tests (P < 0.05). Results: All files resulted in apical extrusion of debris. Reciproc caused significantly less debris extrusion compared to D-RaCe and EdgeFile XR (P < 0.05). Conclusions: The findings revealed that during endodontic retreatment, number, and taper of files might have an influence on the amount of apically extruded debris during endodontic retreatment.PubMedScopu
Effect of powder-to-water ratio on the push-out bond strength of white mineral trioxide aggregate
No full textAimTo evaluate the effect of powder-to-water ratio on the retention characteristics of white mineral trioxide aggregate (MTA; Angelus, Londrina, Pr, Brazil) over time
Diyabet Hastalarında Mineral Trioksit Agregat/1α,25-Dihidroksivitamin D3 Kombinasyonu İle Dental Pulpa Kök Hücrelerinde Odontojenik Farklılaşmanın Araştırılması
No full textDental treatments in diabetic patients have been reported to fail in the long term and these patients have more tooth extraction. It is aimed to investigate the odonto/osteogenic differentiation potential of the combination of Vitamin D3, which is known to play an important role in the mineralization mechanism and is frequently deficient in diabetic patients, with the mineral trioxide aggregate (MTA) used in routine dental treatments, on dental pulp stem cells of diabetic patients. The specificity of mesenchymal stem cells isolated from dental pulps of three non-smoking patients with Type 1 diabetes who did not use drugs other than insulin and three non-smoking controls was evaluated by real-time polymerase chain reaction using CD34, CD45, Nanog Oct-4, CD73, CD90 and CD106 markers. Cells were exposed to four different media: normal medium (NM): DMEM, 10% FBS, antibiotic and antimycotic, differentiation medium (DM): NM plus ß-glycerophosphate and ascorbic acid, DM + MTA: DM plus 0.02mg/mL MTA and DM+ MTA + VitD3: FB plus MTA plus 10nM VitD3. At the end of the 14th day, the odonto/osteogenic differentiation amounts of the cells were evaluated by the alizarin red test, real-time polymerase chain reaction (DSPP, DMP1, COL1α1, OCN) and immunofluorescence (anti-BSP II, anti-DMP1, anti-COL1α1) methods. The mesenchymal stem cell characteristics of the cells were demonstrated by the presence of higher expressions of CD73, CD90 and CD106. Alizarin red staining, expression of odonto/osteogenic markers and immunofluorescence experiments showed that there was more differentiation in controls (p <0.05). In controls, while only MTA provides sufficient effect to induce differentiation, in diabetic patients VitD3 should be added to the medium in order to ensure this effect (p <0.05). Within the limitations of this study, it is recommended to study the combination of MTA with VitD3 in dental treatment of diabetic patients with further experiments and to establish dental treatment procedures considering systemic diseases.Diyabet hastalarında yapılan dental tedavilerin uzun dönemde başarısızlıkla sonuçlandığı ve bu hastalarda daha fazla diş çekimi görüldüğü bilinmektedir. Mineralizasyon mekanizmasında önemli rolü olduğu bilinen ve diyabet hastalarında sıklıkla eksikliği bildirilen Vitamin D3'un rutin diş hekimliği tedavilerinde kullanılan mineral trioksit agregat (MTA) ile kombinasyonunun diyabet hastalarından elde edilen dental pulpa kök hücreleri üzerindeki odonto/osteojenik farklılaştırma potansiyelini araştırmak hedeflenmiştir. Sigara içmeyen, insülin dışında ilaç kullanmayan üç Tip 1 diyabet hastasının ve üç kontrolün dental pulpalarından izole edilen mezenkimal kök hücrelerin özgüllüğü CD34, CD45, Nanog Oct-4, CD73, CD90 ve CD106 belirteçleri kullanılarak gerçek zamanlı polimeraz zincir reaksiyonu ile değerlendirilmiştir. Hücreler dört farklı besi yerine maruz bırakılmıştır: normal besi yeri (NB): DMEM, %10 FBS, antibiyotik ve antimikotik, Farklılaştırma besi yeri (FB): NB’ye ß-gliserofosfat ve askorbik asit, FB+MTA: FB’ye 0,02mg/mL MTA ve FB+MTA+VitD3: FB+MTA besi yerine 10nM VitD3 ilavesini içermektedir. 14. günün sonunda hücrelerin odonto/osteojenik farklılaşma miktarları alizarin kırmızısı testi, gerçek zamanlı polimeraz zincir reaksiyonu (DSPP, DMP1, COL1α1, OCN) ve immünfloresan (anti BSP II, anti DMP1, anti COL1α1) yöntemleri ile değerlendirilmiştir. Hücrelerin mezenkimal kök hücre karakteristiği CD73, CD90 ve CD106 ifadelerinin yüksek bulunması ile gösterilmiştir. Alizarin kırmızısı boyaması, odonto/osteojenik belirteçlerin ifadesi ve immünfloresan deneyleri kontrollerde daha fazla farklılaşma olduğunu göstermiştir (p<0,05). Kontrollerde sadece MTA farklılaşmanın indüklenmesi için yeterli etkinin oluşmasını sağlarken, diyabet hastalarında bu etkinin oluşmasının sağlanması için besi yerine VitD3 ilave edilmelidir (p<0,05). Çalışmanın sınırlamaları dahilinde diyabet hastalarının dental tedavilerinde MTA’nın VitD3 ile kombinasyonunun daha ileri deneyler ile çalışılması ve sistemik hastalıklar göz önünde bulundurularak dental tedavi prosedürlerinin oluşturulması önerilmektedir
Is Pulp Inflammation a Prerequisite for Pulp Healing and Regeneration?
No full textThe importance of inflammation has been underestimated in pulpal healing, and in the past, it has been considered only as an undesirable effect. Associated with moderate inflammation, necrosis includes pyroptosis, apoptosis, and nemosis. There are now evidences that inflammation is a prerequisite for pulp healing, with series of events ahead of regeneration. Immunocompetent cells are recruited in the apical part. They slide along the root and migrate toward the crown. Due to the high alkalinity of the capping agent, pulp cells display mild inflammation, proliferate, and increase in number and size and initiate mineralization. Pulp fibroblasts become odontoblast-like cells producing type I collagen, alkaline phosphatase, and SPARC/osteonectin. Molecules of the SIBLING family, matrix metalloproteinases, and vascular and nerve mediators are also implicated in the formation of a reparative dentinal bridge, osteo/orthodentin closing the pulp exposure. Beneath a calciotraumatic line, a thin layer identified as reactionary dentin underlines the periphery of the pulp chamber. Inflammatory and/or noninflammatory processes contribute to produce a reparative dentinal bridge closing the pulp exposure, with minute canaliculi and large tunnel defects. Depending on the form and severity of the inflammatory and noninflammatory processes, and according to the capping agent, pulp reactions are induced specifically
Comparative evaluation of push-out bond strength of Neo MTA Plus with Biodentine and white ProRoot MTA
No full textObjective: The aim of this in vitro study was to evaluate the bond strength of a new calcium silicate cement, Neo MTA Plus (Avalon Biomed Inc. Bradenton, FL, U.S.A) by comparing ProRoot MTA and Biodentine
The Effect of Increased Temperatures of QMix and EDTA on the Push-out Bond Strength of an Epoxy-resin Based Sealer
No full textBackground: Bond strength of root canal sealers is mainly influenced by the surface tension of the root canal wall. Heat, or the use of a surfactant might reduce surface tension and it is known that surface tension of an EDTA solution significantly affected from temperature rise. QMix is a novel endodontic irrigant for smear layer removal containing EDTA, chlorhexidine and a detergent. There is no data regarding the influence of the temperature of the EDTA and QMix on the push-out bond strength of root canal sealers
Effect of Calcium Hydroxide Dressing on the Dentinal Tubule Penetration of 2 Different Root Canal Sealers: A Confocal Laser Scanning Microscopic Study
No full textIntroduction: The purpose of this study was to evaluate the effect of calcium hydroxide (Ca[OH](2)) dressing on the dentinal tubule penetration of epoxy resin based sealer (AH 26; Dentsply Maillefer, Ballaigues, Switzerland) and tricalcium silicate based sealer (BioRoot RCS; Septodont, Saint Maurdes Fosses, France). Methods: Fifty-two single -rooted mandibular premolars were used. Four samples were assigned as the positive control. Twenty-four samples received Ca(OH)(2) labeled with rhodamine B, whereas the rest did not. Ca(OH)(2) was removed with passive ultrasonic activation and copious irrigation 2 weeks later. Samples were further subdivided into 2 groups, and root canal fillings were performed with a single ProTaper F4 gutta-percha cone (Dentsply Maillefer) combined with 1 of the tested sealers labeled with fluorescein green. After 2 weeks, samples were transversely sectioned at the apical, middle, and coronal levels. The penetration depth and percentage were evaluated via imaging software. Statistical analysis was performed using KruskalWallis, Siegel Castellan post hoc, and Mann -Whitney U tests at P = .05. Results: The mean dentinal tubule penetration depth and percentage values were lowest in the apical third for both sealers. BioRoot RCS showed higher penetrability in all thirds compared with AH 26 (P .05). Conclusions: Passive ultrasonic activation and copious irrigation were insufficient in removing Ca(OH)(2) from root canals. BioRoot RCS presented higher dentinal tubule penetration than AH 26 even in the presence of Ca(OH)(2) residues. Ca(OH)(2) remnants decreased both dentinal tubule penetration depth and the percentage of the tested sealers; however, a more drastic effect was observed for AH 26
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