209 research outputs found

    The AGROVOC concept server: rationale, goals and usage

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    The main objective of the AGROVOC Concept Server (CS)is to create a collaborative reference platform and a one-stop shop for a pool of commonly used concepts related to agriculture, containing terms, definitions and relationships between terms in multiple languages derived from various sources. This paper aims to address the issues

    Keizer et al. "Live-cell micromanipulation of a genomic locus reveals interphase chromatin mechanics" – Data, software and documentation (9/16)

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    Data, software and documentation to reproduce the results presented in [Keizer et al. (2022) ‘Live-cell micromanipulation of a genomic locus reveals interphase chromatin mechanics’ Science, 377:6605, DOI: 10.1126/science.abi9810]. Description Location Centralized GitHub repository with: Local copy of all the code and trajectory/force files Jupyter notebooks to make all the graphs in Keizer et al. Pointers to all the datasets also shown in this table Keizer et al. repository Raw microscopy data: Experiments performed with the 30’-PR scheme Experiment performed with the 100”-PR scheme Experiment performed with high frame rate (dt = 0.5”) Zenodo 1 (30’-PR) Zenodo 2 (30’-PR) Zenodo 3 (30’-PR) Zenodo 4 (30’-PR) Zenodo 5 (30’-PR) Zenodo 6 (100”-PR) Zenodo 7 (30’-PR) Zenodo 8 (30’-PR) Zenodo 9 (dt = 0.5") Zenodo 10 (30’-PR) Concatenated TIFFs and timestamp files for all of the 30’-PR data. Zenodo 11 (1/2) Zenodo 12 (2/2) Python pipeline to generate (i) concatenated movies, (ii) cropped and rotated movies for each cell, and (iii) force time profiles for each cell. ChroMag-pipeline repository Final registered and rotated TIFF files: 30’-PR experiments: n = 35 cells 100”-PR experiment, including time projections & kymograph dt = 0.5” experiments: n = 3 cells no force: n = 11 cells before manipulation, n = 8 cells after manipulation Data files with trajectories and force time profiles for all analyzed cells Instructions and Fiji/Python scripts to reproduce these files. Zenodo 13 Single-MNPs fluorescence: raw data, Python/Fiji scripts and instructions Zenodo 14 MagSim, Python library for magnetic simulations Jupyter notebook for calibrating and generating maps (Fig. S5 & Fig. S6). MagSim repository Force calibration – Method 1: Gradient of free GFP-ferritin in solution Raw microscopy data (6 pillars; Fig. S6B-C) Calculated force maps, with Fiji scripts and instructions to generate them. Zenodo 15 Force calibration – Method 2: Attraction of ferritin-coated beads (Fig. S7) Raw microscopy data (free diffusion and attraction) Python/Fiji scripts to calculate forces. Zenodo 16 Python library for force inference using different polymer models rouselib repository License: All the code, data and documentation in this repository is under GPLv3 license. The Author Accepted Manuscript of the study [Keizer et al. 2022] is under CC-BY 4.0 license. The Final Published Version, published by AAAS, is not (more information). Overview of the raw data repositories (Zenodo 1-10) Refer to the Material and Methods section of the article for details on data production. Each Zenodo dataset represents one day of acquisition. It includes the data that was not retained for further downstream analysis. Each dataset contains: The raw MicroManager folder architecture (one folder contains multiple positions on the coverslip). On occasions where placement or removal of the external magnet led to a loss of focus, the acquisition was stopped and restarted, creating a new MicroManager folder each time. For instance: The various positions were imaged before injection (folder with the _preInjection, _1-pre-inj or _1-inj_1 suffix) These positions were imaged again after injection (suffix _postInjection, _2-post-inj or _1-inj_2) and before the magnet was added (suffix _beforeexp or _before-attr) They were imaged again with the magnet added (suffix _attraction1). If acquisition was stopped and restarted an extra folder is created (suffix _attraction2) They were then imaged after the magnet was removed (suffix _release1) Finally, the cells were monitored after the experiment (suffix _after-exp or _postexp) A text file named lab_journal_[...].txt contains extra information the acquisition and experimental procedure Note: the MicroManager metadata in the TIFF file are fully populated Overview of the concatenated datasets (Zenodo 11-12) In these Zenodo repository, each position (acquired in different folders), is concatenated into a single TIFF movie using code available in the ChroMag-pipeline repository. The folder contains: One TIFF file per selected position One .xls file per selected position, with one line per frame, and columns with the following information: path (Relative path): Reference to the original (raw MicroManager) file start_time (Timestamp): Timestamp saved by MicroManager when the acquisition was started (the «acquire » button was pressed). time_in_file (seconds): Number of seconds between start_time and the acquisition of the current timepoint start_time_s (seconds): Variable start_time converted to a number of seconds time (seconds): Sum of start_time and time_in_file timestamp (Timestamp): Variable time, back-converted to a timestamp timeOn (Timestamp): Time(s) when the magnet was added. This timestamp is provided in the datasets.cfg file in the github repository chromag-pipeline timeOff (Timestamp): Time(s) when the magnet was removed. This timestamp is provided in the datasets.cfg file in the github repository chromag-pipeline forceActivated (Boolean): If the magnet is present during the current frame (calculated from timeOn and timeOff) seconds_since_first_magnet_ON (seconds): Number of (relative) seconds since the magnet was added for the first time. Frame (Integer) Frame number (1-indexed) Positions (Integer): The position number Processed datasets (Zenodo 13) and calibration datasets (Zenodo 14-16) These datasets and their analysis are fully described in the Materials and Methods section of the article and in the different README.md files within the various folders of the datasets.This work received funding from: • the LabEx CELL(N)SCALE (ANR-11-LABX-0038, ANR-10-IDEX-0001-02) (MD, DF, AC) • the Agence Nationale de la Recherche (project CHROMAG, ANR-18-CE12-0023-01) (MD, AC), • the PRESTIGE program of Campus France (PRESTIGE-2018-1-0023) (VK) • the ATIP-Avenir program of CNRS and INERM, the Plan Cancer of the French ministry for research and health (AC, DF), • the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No 757956) (AC), • the LabEx DEEP (ANR-11-LABX-0044, ANR-10-IDEX-0001-02) (AC), • the program Fondation ARC (grant agreement PJA 20161204869) (AC) • the Institut Curie (DF, AC) • the Centre National de la Recherche Scientifique (CNRS) (AC, DF) • the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 666003 (SH) • the NIH GM114190 grant (LAM), • the MIT-France Seed Fund (LAM, MD), • LAM is a recipient of Chaire Blaise Pascal by Île-de-France Administration (LAM)

    Freon-12

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    Document(en) uit de collectie Chemische Procestechnologie.DelftChemTechApplied Science

    Het comfort van de trein

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    Singlet and triplet supercurrents in disordered mesoscopic systems

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    This thesis describes a series of experiments and a theoretical study in order to understand and control the behavior of electrons in many-body systems. In particular, the experiments concentrate on competition between the - antagonistic - electronic states of superconductivity and ferromagnetism, and are strongly motivated by the recent proposal of a new type of superconductivity which appears to defy the Pauli principle, if defined in the usual sense. As the central ingredient of our experiments, we use high-quality thin films of half-metallic Chromiumdioxide (or CrO2), best known for its large scale application in the magnetic recording industry, with an experimentally verified close to 100% spin-polarization. We start the experiments with a magnetotransport study of the magnetic properties of these films, and find a Planar Hall effect which demonstrates that the films, which are ordinarily supposed to have a single easy axis in the plane of the film, instead possess a biaxial magnetic anisotropy, which is analyzed in detail. We then contact the CrO2 films to a conventional (s-wave singlet) superconductor, using microfabrication techniques, and show that superconducting correlations originating in the superconductor, enter the CrO2 and persist over distances incompatible with spin singlet and ballistic transport, thereby providing strong evidence for the presence of s-wave triplet correlations. Moreover, from an application point of view, we show that the superconductor-half-metal-superconductor devices we have used to measure this effect are superconducting transistors that can be switched "on" and "off" with the magnetic field. The theoretical part focusses on the influence of voltages, rather than currents or temperature, on the collapse of the superconducting state in a short mesoscopic superconducting wire between normal metal contacts. It is shown that the collapse of the superconducting state is driven by the applied voltage, and not by the resulting currentApplied Science

    To help kids, parents must do more than censor books

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    In this editorial, the author supports parents\u27 rights to censor what their children read but thinks they infringe on the rights of others when they attempt to restrict what others read. The author applauds the Salem-Keizer School District for its policy regarding reviewing challenged books and allowing alternate assigned readings for children

    Achieving OAI PMH compliancy for CDS/ISIS databases

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    Purpose – The main purpose of this paper is to present the work recently carried out by the Food and Agriculture Organization of the United Nations (FAO) in collaboration with Associazione per la documentazione le biblioteche e gli archivi (DBA) in Italy to make web CDS/ISIS-based applications compliant with the OAI-PMH. CDS/ISIS is an Integrated Storage and Information Retrieval System of Unesco, which is widely used especially in Latin America and Africa. There are hundreds of CDS/ISIS-based application systems managing bibliographical reference, ensuring high quality content through the use of built-in authority files, data entry guidelines and validations. It also allows for metadata export in many different formats. Design/methodology/approach – The methodology adopted included study, analysis and evaluation of three existing solutions for exposing metadata from the CDS/ISIS database repositories to the OAI framework. Findings – The implementation did not include the development of automatic procedures for incremental harvesting from CDS/ISIS databases nor the normalization of the harvested data. However, a lot of experience in implementation of OAI was gained which will be useful for future development of non-CDS/ISIS systems. Research limitations/implications – The research and development work demonstrates the importance and implications of this work for the whole CDS/ISIS community and specifically for the participating centres from the AGRIS network. Originality/value – It proposes an open source, easily parametrizable plug-in tool, which can be adapted to expose metadata from a general structure CDS/ISIS database using the OAI-PMH protocol. This work assures that semantically rich metadata for agricultural science and research publications based on the “AGRIS Application Profile” can be handled by the OAI protocol. This in turn allows for further creation of additional services based on the exchange of knowledge on agricultural science and technology publications world-wide
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