109 research outputs found
De novo designed protein-protein interaction domains for synthetic biology applications in cells
No full textConsent and meaningful experiences at regional Burning Man events
No full textWithin the field of leisure and leisure travel (tourism), consent is often couched inside the idea of free choice. Participants in an activity must freely choose that activity for it be considered leisure, and they must willingly choose to travel for it to be considered tourism. But what about consent during a leisure experience or during leisure travel? This project sought to explore the impact of consent culture on the experiences of participants at Regional Burning Man Events (Burns). The study expanded the tourism studies literature about the importance of consent on tourists’ experiences and further validates the applicability of Maslow’s hierarchy of needs as a measure for studying tourist motivation. In the end, the results of this study show that tourists who attend regional Burns find belonging and a space to explore identity and self expression. By all accounts, a culture of consent at these events heavily contributes to creating an environment where these experiences are possible. Consent takes into account the relational nature of interactions within communities and during leisure activities. As the participants in this study repeated and my own field experience showed, consent is deeply rooted in community. The culture of consent at Burns, whether enacted through formal training or through word of mouth and peer-led enforcement in the rest of the community, is in contrast to the rape culture of the everyday, or as Burners call it, “default” world. It is this culture of consent, one intentionally built to empower individuals and encourage open communication between participants, that enables traditionally vulnerable individuals to have “better” tourism experiences, ones in which their self-esteem and self-actualization needs are met.Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2019-08-01The student, Caitlin Edwards, accepted the attached license on 2017-07-19 at 14:21.The student, Caitlin Edwards, submitted this Thesis for approval on 2017-07-19 at 14:27.This Thesis was approved for publication on 2017-07-20 at 16:13.DSpace SAF Submission Ingestion Package generated from Vireo submission #11536 on 2018-03-02 at 13:02:43Made available in DSpace on 2018-03-02T19:59:47Z (GMT). No. of bitstreams: 2
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Previous issue date: 2017-07-20Embargo set by: Seth Robbins for item 105083
Lift date: 2020-03-02T19:59:52Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 105083
Lift date: 2020-03-02T20:02:46Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemU of I Only Restriction Lifted for Item 105083 on 2020-03-03T10:15:22Z
Exercise science depression studies: A cultural, interpretive, and science studies perspective
No full textThis dissertation takes an interdisciplinary approach to exercise science research on depression, providing a cultural interpretive and science studies perspective. I locate this work at the intersections of multiple fields including kinesiology, sport sociology, disability studies, body studies, and science and technology studies (STS). I analyzed 13 meta-analyses and systematic reviews focused on exercise interventions for depression, published between 2013-2017. Based on my findings, I challenge exercise science’s framing of depression as a costly societal burden, exercise as prescription drug, and the use of prescreening tools as technologies for identifying and rating depressive symptoms. This is particularly relevant given that the World Health Organization listed depression as its top priority for 2017. There is an increased interest in exercise prescription, thus a sociocultural contribution is warranted. I argue that the promotion of exercise as treatment for depression requires more unpacking from a critical cultural perspective. Such an analysis does not aim to delegitimate the appropriateness of exercise interventions. Rather, I am interested in understanding the arguments made by researchers regarding the relationship between exercise and depression. I am primarily interested in studying how exercise science depression studies view depression through their descriptions, definition choices, and proposed relationships between physical activity and depression. My findings offer further insight into the validity of those claims as well as suggestions for ways to modify or add to the design and methodology for future research on this topic and other similar topics. Based on my findings, particularly in relation to the framework of depression as a societal burden and the uncritical and uncontextualized use of clinical prescreening tools, I argue that exercise science must be wary of promoting affective hardening through exercise prescription. I argue that this notion of affective hardening is an extension of historic attempts to harden bodies through physical activity, rooted in ableism and stigma. I acknowledge that we in exercise science operate within a larger social structure. I use my findings to highlight the ways in which research publications in exercise science depression studies help to create and maintain the social structure within which we operate. I conclude by advocating select alternative socioculturally based frameworks that better incorporate empathy for people with mental illness; such frameworks are based in some disability studies the sociology of mental illness.Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2021-05-01The student, Caitlin Clarke, accepted the attached license on 2019-04-14 at 22:09.The student, Caitlin Clarke, submitted this Dissertation for approval on 2019-04-14 at 22:17.This Dissertation was approved for publication on 2019-04-17 at 07:55.DSpace SAF Submission Ingestion Package generated from Vireo submission #13615 on 2019-08-22 at 16:21:21Made available in DSpace on 2019-08-23T20:47:23Z (GMT). No. of bitstreams: 3
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Previous issue date: 2019-04-17Embargo set by: Seth Robbins for item 112314
Lift date: 2021-08-23T20:47:38Z
Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 112314
Lift date: 2021-08-23T20:48:32Z
Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemLimited Restriction Lifted for Item 112314 on 2021-08-24T09:15:38Z
Rewilding Time in the Vale do Côa
No full textThis is the author accepted manuscript. The final version is available from Bloomsbury via the link in this recordArts and Humanities Research Council (AHRC
Metal Ions, Not Metal-Catalyzed Oxidative Stress, Cause Clay Leachate Antibacterial Activity
No full textabstract: Aqueous leachates prepared from natural antibacterial clays, arbitrarily designated CB-L, release metal ions into suspension, have a low pH (3.4–5), generate reactive oxygen species (ROS) and H[subscript 2]O[subscript 2], and have a high oxidation-reduction potential. To isolate the role of pH in the antibacterial activity of CB clay mixtures, we exposed three different strains of Escherichia coli O157:H7 to 10% clay suspensions. The clay suspension completely killed acid-sensitive and acid-tolerant E. coli O157:H7 strains, whereas incubation in a low-pH buffer resulted in a minimal decrease in viability, demonstrating that low pH alone does not mediate antibacterial activity. The prevailing hypothesis is that metal ions participate in redox cycling and produce ROS, leading to oxidative damage to macromolecules and resulting in cellular death. However, E. coli cells showed no increase in DNA or protein oxidative lesions and a slight increase in lipid peroxidation following exposure to the antibacterial leachate. Further, supplementation with numerous ROS scavengers eliminated lipid peroxidation, but did not rescue the cells from CB-L-mediated killing. In contrast, supplementing CB-L with EDTA, a broad-spectrum metal chelator, reduced killing. Finally, CB-L was equally lethal to cells in an anoxic environment as compared to the aerobic environment. Thus, ROS were not required for lethal activity and did not contribute to toxicity of CB-L. We conclude that clay-mediated killing was not due to oxidative damage, but rather, was due to toxicity associated directly with released metal ions.The article is available at http://journals.plos.org/plosone/article?id=10.1371/journal.pone.011517
2008 Author Recognition Bibliography
No full texthttps://scholarworks.gvsu.edu/authorrecognition/1006/thumbnail.jp
Robust de novo designed homotetrameric coiled coils
No full textDe novo designed protein domains are increasingly being applied in biotechnology, cell biology and synthetic biology. Therefore, it is imperative that these proteins are robust to superficial changes, i.e., small changes to their amino-acid sequences should not cause gross structural changes. In turn, this allows properties such as stability and solubility to be tuned without affecting structural attributes like tertiary fold and quaternary interactions. Reliably designed proteins with predictable behaviors may then be used as scaffolds to incorporate function, e.g., through the introduction of features for small-molecule, metal or macromolecular binding, and enzyme-like active sites. Generally, achieving this requires the starting protein fold to be well understood. Herein, we focus on designing α-helical coiled coils, which are well studied, widespread and often direct protein-protein interactions in natural systems. Our initial investigations reveal that a previously designed parallel, homotetrameric coiled coil, CCTet, is not robust to sequence changes that were anticipated to maintain its structure. Instead, the alterations switch the oligomeric state from tetramer to trimer. To improve the robustness of designed homotetramers, additional sequences based on CC-Tet were produced and characterized in solution and by X-ray crystallography. Of these updated sequences, one is robust to truncation and to changes in surface electrostatics; we call this CC-Tet*. Variants of the general CC-Tet* design provide a set of homotetrameric coiled coils with unfolding temperatures in the range 40 ˚C to >95 ˚C. We anticipate that these will be of use in applications requiring robust and well defined tetramerization domains
Determination of dairy cattle euthanasia criteria and analysis of barriers to humane euthanasia in the United States: Dairy producer surveys and focus groups
No full textThere are currently no clear guidelines in the US and some other countries regarding euthanasia decision making timelines for dairy cattle that become injured or ill to the extent that recovery is unlikely or impossible. Our study aimed to identify decision making criteria and the most common factors considered when making and carrying out euthanasia decisions. Dairy producers were recruited to participate in a mailed survey (Part I, 307 completed surveys were returned) or in one of three focus groups (Part II, 8-10 producers/group, n = 24). Part I (survey): Farm owners were most commonly responsible for on-farm euthanasia and most respondents would treat and monitor compromised cattle for a majority of 15 health conditions. Responses were highly variable; for example, 6.3% and 11.7% of respondents would never euthanize a non-ambulatory cow or calf, respectively. Part II (focus groups): Three main themes (animal, human, and farm operation) were identified from discussion which focused primarily on animal welfare (16% of the discussion) and human psychology (16%). Participants expressed a desire to eliminate animal suffering by euthanizing, alongside a wide range of emotional states. Development of specific standards for euthanasia is a critical next step and more research is needed to understand the human emotions surrounding euthanasia decision making.United States Department of Agricultur
Author Correction: Loss of Phd2 cooperates with BRAFV600E to drive melanomagenesis
No full textThe original version of this Article contained an error in the spelling of the author Brett L. Ecker, which was incorrectly given as Brett Ecker. This has now been corrected in both the PDF and HTML versions of the Article.published_or_final_versio
ENDING IMPUNITY: CRITICAL REFLECTIONS ON THE PROSECUTION OF HEADS OF STATE
No full text Since 1990, nearly seventy heads of state have been prosecuted for crimes committed while in office. From a legal perspective, the prosecutions have been possible largely due to an erosion of the state immunity principle and the emergence of specialized courts with a mandate to prosecute political figures. Interesting questions arise as to why the legal landscape shifted and whether the trend towards greater numbers of prosecutions has enhanced the rule of law. In their book, Prosecuting Heads of State, editors Ellen L Lutz and Caitlin Reiger shed light on these larger questions. In this review, the author explores Lutz and Reiger's premise that the increased willingness to prosecute heads of state has been the direct result of activist lawyering, and questions whether the rule of law can truly be enhanced by prosecutions that are inextricably linked to political motivations. The author concludes by cautioning against the over-reliance on judicial accountability mechanisms in transitional justice contexts. </jats:p
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