178 research outputs found
Programmed exosome fusion for energy generation in living cells
No full text© 2021, The Author(s), under exclusive licence to Springer Nature Limited.Biological membrane-enclosed organelles are fascinating examples of spatially confined nanoreactors for biocatalytic transformations such as cascade reactions involving multiple enzymes; however, the fabrication of their synthetic mimics remains a considerable challenge. Here we demonstrate supramolecular chemistry-based bridging of two membranes leading to controlled fusion of exosomes that act as nanoreactors for effective biocatalytic cascades, with prolonged functionality inside of living cells. Exosome membrane proteins were chemically engineered with a catechol moiety to drive fusion by supramolecular complexation to bridge the membranes. This strategy successfully encapsulated multiple enzymes and assembled the minimal electron transport chain in the plasma membrane, leading to tuneable, enhanced catalytic cascade activity capable of ATP synthesis inside of tissue spheroids. This nanoreactor was functional for many hours after uptake into living cells, showed successful penetration into tissue spheroids and repaired the damaged region by supplying ATP, all of which represent an advance in the mimicking of nature’s own organelles. [Figure not available: see fulltext.].11Nsciescopu
A fidget spinner for the point-of-care diagnosis of urinary tract infection
No full text© 2020, The Author(s), under exclusive licence to Springer Nature Limited.The point-of-care detection of pathogens in biological samples in resource-limited settings should be inexpensive, rapid, portable, simple and accurate. Here, we describe a custom-made fidget spinner that rapidly concentrates pathogens in 1-ml samples of undiluted urine by more than 100-fold for the on-device colorimetric detection of bacterial load and pathogen identification. In Tiruchirappalli, India, the device enabled the on-site detection of infection with the naked eye within 50 min in urine samples from 39 patients suspected of having a urinary tract infection. We also show that, in 30 clinical samples of urinary tract infection, the device can be used to perform an antimicrobial susceptibility test for the antimicrobial drugs ciprofloxacin and cefazolin within 120 min. The fidget spinner could be used in low-resource settings as an inexpensive handheld point-of-care device for the rapid concentration and detection of pathogens in urine sample
Expanding Dimensions Of Test Management For Object Oriented Software Author – Sumit Kumar
No full textThe role of Test Management in the software industry has not remained the same. Few years back, testing was rarely present in the checklist of Project Manager. The strategic elements of business have guided software industry towards the realization of impact of quality in this industry. The growing complexity of today’s application, combined with increased pressure and skyrocketing costs of application failure and downtime have catapulted the need for a process, which can be effectively used for achieving desired quality level. Evolution of Test Management as a process has provided the solution to the industry. Ground rules have been laid down under Test Management process and organizations are trying to synchronize this process with their business goals by selecting the most suitable Test Model. But, in this race for Quality, an important aspect of testing has been ignored. Present Test Management process has been identified and established at syntactic level where emphasis is on testing the code. The growing need of Quality in busines
New Realizations of Efficient and Secure Private Set Intersection Protocols Preserving Fairness
No full textCoupled Halide-deficient and Halide-rich Reaction System for Doping in Perovskite Armed Nanostructures
No full textInsights of Mn(II) doping in CsPbCl3-armed hexapod nanostructures is reported. These complex structures were typically formed in halide concentration tuned modulated reactions. Cores were first formed under halide deficient condition and with enriching halides; these were transformed to armed structures. Doping of Mn(II) was observed facilitated during the arm growth in the second stage of the reaction. These observations were supported with decoupled reactions with minimized and maximized halide concentrations carried out in separate reactions. However, less interference for the exciton to dopant energy transfer was noticed for the defect states created in halide-deficient medium, and the intensity of the dopant emission remained proportional to the amount of dopant inserted in the nanocrystals. Being this is an in situ observation in the coupled reactions of both poor and rich halide reaction systems, the finding would strengthen the understanding of doping in perovskite host nanocrystals
Fair mPSI and mPSI-CA: Efficient Constructions in Prime Order Groups with Security in the Standard Model against Malicious Adversary
No full textIn this paper, we propose a construction of fair and efficient mutual Private Set Intersection (mPSI) with linear communication and computation complexities, where the underlying group is of prime order. The main tools in our approach include: (i) ElGamal and Distributed ElGamal Cryptosystems as multiplicatively Homomorphic encryptions, (ii) Cramer-Shoup Cryptosystem as Verifiable encryption. Our mPSI is secure in standard model against malicious parties under Decisional Diffie-Hellman (DDH) assumption. Fairness is achieved using an off-line semi-trusted arbiter. Further, we extend our mPSI to mutual Private Set Intersection Cardinality (mPSI-CA) retaining all the security properties of mPSI. More interestingly, our mPSI-CA is the first fair mPSI-CA with linear complexity
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