1,721,065 research outputs found
Proceedings SPRAY 2012, 21.-22. Mai 2012, Berlin
No full textSPRAY 2012, 21.-22. Mai 2012, Berlin Die Spray-Workshops sollen Ingenieuren und Wissenschaftlern aus Forschung und Industrie Gelegenheit bieten, sich - über ihre jeweiligen Einsatzgrenzen hinweg - mit aktuellen Problemen und Lösungsansätzen der Fluidzerstäubung, den entsprechenden Messtechniken sowie der physikalischen Modellbildung von Sprayprozessen und deren numerischer Behandlung auseinander zu setzen. Die Spray-Workshops werden seit 1994 in loser Reihenfolge im deutschsprachigen Raum durchgeführt. Der aktuelle Workshop Spray 2012 ist der 10. in dieser Reihe. In den aktuellen Spray-Workshop integriert ist das Jahreskolloquium des DFG-SPP Prozess-Spray: Herstellen funktionaler Feststoffpartikeln in Sprühverfahren von den Anforderungen an das Pulver und an seine Eigenschaften zum geeigneten Prozess. Durch diese Einbindung soll das Thema der Fluidzerstäubung und seine aktuellen Entwicklungen und der Fortschritt im SPP in breiter Anwendung vorgestellt und diskutiert werden. Die Proceedings zu diesem Workshop mit den eingereichten Tagungsbeiträgen sind auf CD erschienen und werden nach dem Modus des Open Access auf dem E-Lib Server der Staats- und Universitätsbibliothek der Universität Bremen elektronisch publiziert. Sie sind damit dauerhaft und stabil archiviert und werden über eine dauerhaft verfügbare Internetadresse mittels URN (uniform resource name) zitierfähig. S. Drusch / U. Fritschin
Site-directed spin labeling for the evaluation of amyloid and non-amyloid aggregation mechanisms of β-lactoglobulin using electron paramagnetic resonance spectroscopy and mass spectrometry
Full text linkThe approach of site-directed spin labeling can be implemented for the food protein β lactoglobulin (β lg), by adjusting the pH value and the spin label/β lg ratio, although it contains five cysteine residues, and used to characterize the amyloid aggregation mechanism of β lg. Further, the surface activity of the amyloid aggregate system is determined by the smaller aggregates, peptides, and monomeric sized protein of the non-amyloid fraction due to faster migration to the interface and a higher adsorption rate. The results of this work are mainly based on measurements using electron spin resonance spectroscopy and mass spectrometry.
MTSSL and IPSL bind covalently and reproducibly to all five cysteine residues with different preferences. The best labelling conditions for MTSSL are pH 7.5 and an equimolar MTSSL/β-lg ratio. Whereby MTSSL dimers are already formed at a pH value of 7.5. The bonding of the spin labels to β-lg is temperature (90°C) and acid (pH 2) resistant, whereas it is assumed the biradicals are reduced to monoradical dimers during heat incubation.
Spin labeling with IPSL reduces the intra- and intermolecular β-sheets at pH 3.5. However, SDSL does not change the morphology and building blocks of amyloid aggregates but interferes with the thioflavin-T measurement. Impact of the label on the secondary protein structure is lower for MTSSL compared to IPSL because of its flexible bond.
MTSSL is most sensitive to changes in the arrangement of β-sheets, whereas IPSL is most sensitive to changes in random coil at position Cys160. The amyloid aggregates formed at pH 3.5 could be characterized using SDSL. The monomer-like β-lg aggregates had the form of a pearl chain. However, the spin labeled side chains were not incorporated into amyloid structures formed at pH 2.
Fibrils and non-amyloid material act synergistically at the air-water interface because non-amyloid material is embedded into fibrillar network. But the adsorption behavior of the amyloid aggregate system is dominated by the non-amyloid fraction. Only small amounts of amyloid aggregates were necessary to stabilize foams. The airflow used for foaming resulting in gas bubbles caused sufficient shearing to partly degrade fibrils
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Schäume als neue Träger-Vehikel für die topische Applikation
Full text linkFoams are relatively new delivery systems for topical administration. However, characterization of foams is only pooröy described in Pharmacopeias. A comprehensive approach to characterize foams has therefore been developed. Additionally, different formulation platforms were developed and investigated for their different release behaviour.Schäume sind relativ neue Freigabesysteme, deren Charakterisierung allerdings in den Arzneibüchern nur unzureichend beschrieben ist. Daher wurden Methoden entwickelt, um Schaumprodukte umfassend zu charakterisieren. Weiterhin wurde eine Formulierungsplattform entwickelt für Aerosol- und Non-Aerosolschäume und deren Einfluss auf die Wirkstofffreigabe untersucht
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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