79 research outputs found
sj-docx-1-cll-10.1177_09636897231207177 – Supplemental material for Timing of Mesenchymal Stromal Cell Therapy Defines its Immunosuppressive Effects in a Rat Lung Transplantation Model
No full textSupplemental material, sj-docx-1-cll-10.1177_09636897231207177 for Timing of Mesenchymal Stromal Cell Therapy Defines its Immunosuppressive Effects in a Rat Lung Transplantation Model by Yukinori Tanoue, Tomoshi Tsuchiya, Takuro Miyazaki, Mayumi Iwatake, Hironosuke Watanabe, Hiroshi Yukawa, Kazuhide Sato, Go Hatachi, Koichiro Shimoyama, Keitaro Matsumoto, Ryoichiro Doi, Koichi Tomoshige and Takeshi Nagayasu in Cell Transplantation</p
DSDApp: An Open-Access Tool for Definitive Screening Design
No full textDefinitive Screening Design (DSD) is a powerful design of experiment technique employed to find significant factors (parameters) and optimize the factor levels in many experiments of different fields. However, making its design and analyzing the obtained results usually requires expensive software or advance programming knowledge. Therefore, the authors of this work developed a web application, called “DSDApp”, with which users can make designs and analyze the results of DSD without statistical and programming efforts. DSDApp can generate DSD tables, make second-order models, and optimize factor levels to obtain max/min/target output values. Corresponding authors: Ryoichiro Hayasaka, [email protected] Pablo Cayado, [email protected]
The cellular basis of Dictyostelium morphogenesis
No full textOne of the central aims of the study of development is to understand how distinct cellular behaviours, for example, division, differentiation, apoptosis, and movement, are coordinated in space and in time to result in reproducible pattern formation and morphogenesis. Coordination of these cellular behaviours requires extensive communication between cells of different types and between the cells and their environment. The social amoeba Dictyostelium discoideum, a simple genetically tractable organism situated at the threshold of single and multicellular organisms in the evolutionary tree of life, is well suited for the study of these interactions because its genome has been sequenced and it is amenable to experimental manipulation through targeted gene disruption and replacement (Kreppel, Fey et al. 2004; Eichinger, Pachebat et al. 2005). Dictyostelium cells normally live as single cells in the leaf litter of the soil where they feed on bacteria and divide by binary fission. Under starvation conditions, up to several hundred thousand cells aggregate chemotactically to form a multicellular structure the slug, that directed by light and temperature gradients migrates to the surface of the soil to form a fruiting body. The fruiting body is composed of a stalk supporting a mass of spores. The spores are a dormant stage which after dispersal may germinate to release amoebae, thus closing the life cycle (Fig. 1). We describe here key aspects of the signalling mechanisms coordinating cellular behaviours responsible for pattern formation and morphogenesis
Spheroid morphology of lung cancer cell lines correlates with oncological profiles
No full textNagasaki University (長崎大学)博士(医学)We assessed the correlation between Multicellular tumor spheroids (MCTS) morphology and the oncological profile of lung cancer cells. MCTS were generated in five lung cancer cell lines and classified into Type–A MCTS, which showed strong aggregation, and Type–B MCTS, which showed weak aggregation. Drug resistance was higher in Type–A MCTS, and invasive ability was higher in Type–B MCTS. The oncologic profile of lung cancer cell lines correlated with MCTS morphology. MCTS morphology could thus be used in basic oncology research and as a clinical prognostic tool.長崎大学学位論文 学位記番号:博(医歯薬)甲第1708号 学位授与年月日:令和7年6月4日Author: Kaido Oishi, Keitaro Matsumoto, Shintaro Hashimoto, Fumitake Uchida, Ryosuke Hara, Masato Nishimuta, Takamune Matsumoto, Mayumi Iwatake, Koichi Tomoshige, Ryoichiro Doi, Ryusuke Machino, Tomohiro Obata, Takeshi NagayasuCitation: Discover Oncology, 16, art. no. 627: 2025Nagasaki University (長崎大学), 博士(医学) (2025-06-04)doctoral thesi
Adipose-Derived Mesenchymal Stem Cells Attenuate Immune Reactions Against Pig Decellularized Bronchi Engrafted into Rat Tracheal Defects
No full textNagasaki University (長崎大学)博士(医学)Decellularized scaffolds are promising biomaterials for tissue and organ reconstruction; however, strategies to effectively suppress the host immune responses toward these implants, particularly those without chemical crosslinking, remain warranted. Administration of mesenchymal stem cells is effective against immune-mediated inflammatory disorders. Herein, we investigated the effect of isogeneic abdominal adipose-derived mesenchymal stem/stromal cells (ADMSCs) on xenogeneic biomaterial-induced immunoreactions. Peripheral bronchi from pigs, decellularized using a detergent enzymatic method, were engrafted onto tracheal defects of Brown Norway (BN) rats. BN rats were implanted with native pig bronchi (Xenograft group), decellularized pig bronchi (Decellularized Xenograft), or Decellularized Xenograft and ADMSCs (Decellularized Xenograft +ADMSC group). In the latter group, ADMSCs were injected intravenously immediately post implantation. Harvested graft implants were assessed histologically and immunohistochemically. We found that acute rejections were milder in the Decellularized Xenograft and Decellularized Xenograft+ADMSC groups than in the Xenograft group. Mild inflammatory cell infiltration and reduced collagen deposition were observed in the Decellularized Xenograft+ADMSC group. Additionally, ADMSC administration decreased CD8+ lymphocyte counts but increased CD163+ cell counts. In the Decellularized Xenograft+ADMSC group, serum levels of vascular endothelial growth factor and IL-10 were elevated and tissue deposition of IgM and IgG was low. The significant immunosuppressive effects of ADMSCs illustrate their potential use as immunosuppressive agents for xenogeneic biomaterial-based implants.長崎大学学位論文 学位記番号:博(医歯薬)甲第1559号 学位授与年月日:令和5年12月6日Author: Makoto Hisanaga, Tomoshi Tsuchiya, Hironosuke Watanabe, Koichiro Shimoyama, Mayumi Iwatake, Yukinori Tanoue, Keizaburo Maruyama, Hiroshi Yukawa, Kazuhide Sato, Yoshimi Kato, Keitaro Matsumoto, Takuro Miyazaki, Ryoichiro Doi, Koichi Tomoshige & Takeshi NagayasuCitation: Organogenesis, 19(1), art. no. 2212582; 2023Nagasaki University (長崎大学), 博士(医学) (2023-12-06)doctoral thesi
Anti-tumor Effects of Erlotinib via Thymidylate Synthase Down-regulation in Pancreatic Cancer Cells
No full textNagasaki University (長崎大学)博士(医学)Background/Aim: In pancreatic cancer, gemcitabine and EGFR tyrosine kinase inhibitors (EGFR-TKIs) are common chemotherapy options. Reports have shown that EGFR-TKIs suppress the expression of thymidine synthase (TS), an important enzyme for DNA biosynthesis, and increase sensitivity to gemcitabine in lung cancer. However, no such reports have been made in pancreatic cancer. Materials and Methods: Human pancreatic cancer cell lines MiaPaCa2, Panc1, and BxPc3 were used. TS mRNA and protein expression levels in the cells were analyzed after erlotinib treatment. In addition, the anti-tumor effect of TS knockdown was verified using TS siRNA, along with its synergistic effect when combined with gemcitabine. Results: TS expression was high in MiaPaCa2 and Panc1 cells and low in BxPc3 cells. After erlotinib treatment, TS mRNA and protein levels decreased markedly in MiaPaCa2 cells dose-dependently, but not in Panc1 cells. TS siRNA caused specific down-regulation of TS in MiaPaCa2 and Panc1 cells. TS down-regulation resulted in an anti-tumor effect in these cells (MiaPaCa2 42%; Panc1 38%; p<0.05), showing a synergistic effect when combined with gemcitabine. Conclusion: Erlotinib could have a synergistic anti-tumor effect when combined with gemcitabine via down-regulation of TS expression.長崎大学学位論文 学位記番号:博(医歯薬)甲第1664号 学位授与年月日:令和7年3月5日Author: SATOSHI TABUCHI, TETSURO TOMINAGA, TOMOSHI TSUCHIYA, RYOICHIRO DOI, JYUNICHI ARAI, TAKASHI NONAKA, TAKURO MIYAZAKI, TATSUO INAMINE, KAZUHIRO TSUKAMOTO and KEITARO MATSUMOTOCitation: Anticancer Research, 45(2), pp.503-510; 2025Nagasaki University (長崎大学), 博士(医学) (2025-03-05)doctoral thesi
Curved Axillary Incision with Video-Assisted Thoracoscopic Surgery: An Alternative Approach for Teenage Female with Large Apical Chest Wall Tumor
No full textPrimary chest wall tumors are rare, their common clinical features are not well known, and surgical resection remains the main treatment. Apical chest wall tumors require large skin incisions and dissection of the chest wall muscles, making it difficult to maintain cosmetic appearance, respiratory function, and support of the upper extremity. There are few treatment options and no studies have reported on thoracotomy that spares muscles and preserves cosmetic superiority. However, in benign chest wall tumors in young patients, it is necessary to consider radicality, cosmetic superiority, and muscle sparing. We used a combined axillary incision and thoracoscopic approach to treat a massive myxoid neurofibroma at the apical chest wall in a 14-year-old female and were able to preserve the chest wall, upper limb function, and cosmetic aspects. This report provides a detailed description of the combined axillary incision and thoracoscopic approach for apical chest wall tumors.Annals of Thoracic and Cardiovascular Surgery, 30(1), art. no. 23-00189; 202
Curved Axillary Incision with Video-Assisted Thoracoscopic Surgery: An Alternative Approach for Teenage Female with Large Apical Chest Wall Tumor
No full textPrimary chest wall tumors are rare, their common clinical features are not well known, and surgical resection remains the main treatment. Apical chest wall tumors require large skin incisions and dissection of the chest wall muscles, making it difficult to maintain cosmetic appearance, respiratory function, and support of the upper extremity. There are few treatment options and no studies have reported on thoracotomy that spares muscles and preserves cosmetic superiority. However, in benign chest wall tumors in young patients, it is necessary to consider radicality, cosmetic superiority, and muscle sparing. We used a combined axillary incision and thoracoscopic approach to treat a massive myxoid neurofibroma at the apical chest wall in a 14-year-old female and were able to preserve the chest wall, upper limb function, and cosmetic aspects. This report provides a detailed description of the combined axillary incision and thoracoscopic approach for apical chest wall tumors.Annals of Thoracic and Cardiovascular Surgery, 30(1), art. no. 23-00189; 2024journal articl
Surgical treatment for therapy-related pectoral hematoma: report of a case and review of published reports
No full textA 96-year-old man with a rapidly growing right chest wall mass was referred to our department for further treatment. Enhanced chest computed tomography showed a huge pectoral hematoma (12×6 cm) in the right thorax. He was on oral antiplatelet medication, but no abnormalities in clotting ability were detected. Because the hematoma was enlarging and painful, it was evacuated surgically and hemostasis achieved around the pectoral branches of the thoraco-acromial artery. His postoperative course was uneventful with no evidence of subcutaneous fluid retention. Surgical hemostasis and hematoma evacuation of this pectoral hematoma might be effective as one treatment method.Acta medica Nagasakiensia, 65(3), pp.131−133; 202
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