2,137 research outputs found
HERP Insertion Mixture v1
No full textThis is part of the HERP protocol, from: Alexander WG, Doering DT,andHittinger CT (2014) High-Efficiency Genome Editing and Allele Replacement in Prototrophic and Wild Strains of Saccharomyces.Genetics 198:859-866;doi:10.1534/genetics.114.170118 </p
Yeast Extract?Peptone?Glycerol +Antifolates (YPGly+ AF) Media v1
No full textThis recipe is from: Alexander WG, Doering DT,andHittinger CT (2014) High-Efficiency Genome Editing and Allele Replacement in Prototrophic and Wild Strains of Saccharomyces.Genetics 198:859-866;doi:10.1534/genetics.114.170118 See the full protocol for step-by-step YPGly+ AF preparation instructions. </p
HERP: Haploid Engineering and Replacement Protocol for Saccharomyces v1
No full textThis protocol is from: Alexander WG, Doering DT,andHittinger CT (2014) High-Efficiency Genome Editing and Allele Replacement in Prototrophic and Wild Strains of Saccharomyces.Genetics198:859-866;doi:10.1534/genetics.114.170118 Please see the full manuscript for additional details. The purpose of this document is to provide you with an easy‐to‐follow guide to using the HERP cassettes. We will go through the preparation of the selection and counterselection media, the culturing and transformation for insertion of the HERP cassettes, and counterseletive replacement of the HERP cassettes. </p
Self-archiving practice and the influence of publisher policies in the social sciences
No full textAuthors in different disciplines exhibit very different behaviours on the so-called ‘green’ road to open access, i.e. self-archiving. This study looks at the self-archiving behaviour of authors publishing in leading journals in six social science disciplines. It tests the hypothesis that authors are self-archiving according to the norms of their respective disciplines rather than following self-archiving policies of publishers, and that, as a result, they are self-archiving significant numbers of publisher PDF versions. It finds significant levels of
self-archiving, as well as significant self-archiving of
the publisher PDF version, in all the disciplines
investigated. Publishers’ self-archiving policies have
no influence on author self-archiving practice
Digital twin application in heritage facilities management: systematic literature review and future development directions
No full textThis paper aims to investigate the theoretical and practical links between digital twin (DT) application in heritage facilities management (HFM) from a life cycle management perspective and to signpost the future development directions of DT in HFM.This state-of-the-art review was conducted using a systematic literature review method. Inclusive and exclusive criteria were identified and used to retrieve relevant literature from renowned literature databases. Shortlisted publications were analysed using the VOSviewer software and then critically reviewed to reveal the status quo of research in the subject area.The review results show that DT has been mainly adopted to support decision-making on conservation approach and method selection, performance monitoring and prediction, maintenance strategies design and development, and energy evaluation and management. Although many researchers attempted to develop DT models for part of a heritage building at component or system level and test the models using real-life cases, their works were constrained by availability of empirical data. Furthermore, data capture approaches, data acquisition methods and modelling with multi-source data are found to be the existing challenges of DT application in HFM.In a broader sense, this study contributes to the field of engineering, construction and architectural management by providing an overview of how DT has been applied to support management activities throughout the building life cycle. For the HFM practice, a DT-cum-heritage building information modelling (HBIM) framework was developed to illustrate how DT can be integrated with HBIM to facilitate future DT application in HFM. The overall implication of this study is that it reveals the potential of heritage DT in facilitating HFM in the urban development context.Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Design & Construction Managemen
Managing HKU author reputations, enhancing HKU's reputation
No full textThe HKU "Knowledge Exchange" initiative has launched many separate projects that aim to make HKU researchers and their research highly visible. One of these projects is the creation of HKU ResearcherPages in HKU's institutional repository, that highlight the research and achievements of each professoriate staff at HKU, including their authored publications which are freely available in open access. These pages show download counts of these publications, and paper and citations metrics from Scopus and Thomson Reuter's ResearcherID These pages also allow the HKU author to manage, control, and take charge of how his or her digital presence, or reputation, is shown to the world. Newly aware of their digital presence, HKU authors now have the incentive and tools to correct errors, add missing publications, and otherwise burnish their reputation as shown in these HKU ResearcherPages, Scopus, and ResearcherID. The renown of their sponsoring university correspondingly shines brighter; as HKU paper and citation counts are positively amended, offers of contract research and research collaboration increase, and, invitations to speak and consult, appear as if by.., magic.published_or_final_versionThe World 100 Conference: Managing University Reputation in a Competitive World, Hong Kong, 22-23 June 2010
A Conceptual Definition of Quality of Life for People Living with an Implanted, Destination Therapy Left Ventricular Assist Device
No full textBACKGROUND: People with advanced heart failure experience persistently severe symptoms with increasingly impaired capacity and quality of life. Treatment options beyond palliative care are limited to heart transplantation or implantation with a mechanical pump that replaces the weakened heart’s function. The left ventricular assist device (LVAD) serves as a temporary bridge to transplant or bridge to recovery, or a permanent replacement as destination therapy (DT). While DT-LVAD recipients initially accept the device to extend life, there is a gap in understanding how DT-LVAD recipients define a life worth living, and how nursing and the health care team can help reach that goal. PURPOSE: This study’s purpose is to determine the conceptual definition of “quality of life” in people with a DT-LVAD. METHODS: A grounded theory study was conducted using a theoretical sample and open, selected and theoretical coding processes within the constant comparison method. Theoretical saturation was reached with 11 participants (age 32-79 years). RESULTS: Normalizing emerged as the basic social process. Participants reported that a life supported by battery-powered equipment is still a normal, human experience. A process of acceptance and adjustment occurred to Normalize a machine-dependent life. Although extending life was the original LVAD objective, recipient goals and needs evolved over time. Dependence on a machine to extend life did not replace the need for a life worth living. The conceptual definition of quality of life when living with a DT-LVAD emerged as: I am able to live my life and do what I want, with some adjustments. CONCLUSION: This conceptual definition of quality of life from the DT-LVAD recipient perspective enhances the health care team’s understanding and ability to improve DT-LVAD recipient lives. Life with an LVAD is not simply about survival; it is survival of quality that makes a difference and defines success.2021-05-2
Relationships' sustainability: the case of German wheat-to-bread chain
No full textIn recent years there has been a shift in emphasis from transaction-based to more co-operative relationships, as chain members have recognised the need to invest in their supply chain relationships in order to protect their businesses. These non-arm’s-length relationships are capable of generating relational rents for chain partners. The main aim of this paper is to investigate why, in spite of the advantages of the non-arm’s-length relationships, some relationships do not continue. To enhance the understanding of the termination process, we identify and analyse the factors inducing relationship sustainability (continuation) as well as termination in the case of the German wheat-to-bread chain. The study built on the findings of relationship marketing approach that stresses the importance of building longer-term relationships with customers rather than carrying out individual transactions. In addition, the findings of modern microeconomic theories including transaction costs theory and industrial theory are taken into consideration. The empirical analysis is based on two data sets: First, a quantitative questionnaire survey and second, interviews with stakeholders of the wheat-to-bread chain were conducted, aiming at identifying the role of economic and behavioral dimensions of relationships for their termination. The questionnaire survey provides that businesses assess the relationships in the wheat-to-bread chain, being of high quality and long term duration. The results show that trust, satisfaction and commitment - the behavioural dimensions of relationships quality - are very high in the considered chain. Competitive price, competitive quality and supply continuity were identified as determinants mostly important for buyers’ satisfaction as directly influencing relationships’ performance. In addition, the results demonstrate that lack of trust is the reason why relationships do not develop or are terminated in the early phases of the relationship. In the long lasting relationships on the other hand, the reasons for termination are mostly of economic nature. The paper shows why sustainable relationships may enhance business’ competitiveness and analyses why in spite of it the relationships terminate. As the result of the research we understand the sustainable relationships as those relationships in that the costs for starting the relationship are exceeded by returns gained from the cooperation in time.economic relationships, sustainability, termination, Agribusiness,
Improvement of personnel assessment methods in company "DT Mobile"
No full textBakalaura darbā „Personāla novērtēšanas metožu pilnveidošana uzņēmumā „DT Mobile” autors pēta un izstrādā savus priekšlikumus personāla novērtēšanas metožu pilnveidošanai. Uzņēmums ir noteicis jaunus mērķus klientu apkalpošanas līmeņa paaugstināšanai, līdz ar to ir nepieciešams izstrādāt atbilstošas izmaiņas līdzšinējās novērtēšanas metodēs.
Bakalaura darbs veido 3 nodaļas. Pirmajā daļā ir apskatītas personāla novērtēšanas veidi un to definīcijas, mērķi un tiek aprakstītas personāla darbības novērtēšanā izmantojamas metodes, un pielietošanas iespējas, kā arī to trūkumi. Darba otrajā un trešajā nodaļā tiek izpētīta uzņēmumā esošās personāla novērtēšanas sistēmas un analizēti novērtēšanas rādītāji un problēmas novērtēšanas sistēmās. Noslēgumā autors ir apkopojis secinājumus un izstrādājis savus priekšlikumus personāla novērtēšanas metožu pilnveidošanai uzņēmumā „DT Mobile”.
Bakalaura darba apjoms ir 64 lapas, tajās izvietotas 3 nodaļas un 16 attēli, pievienoti 2 pielikumi.In the thesis work “Improvement of personnel assessment methods in company “DT Mobile” the author explores and develops proposals for personnel assessment methods improvement. The company has set the new goals for increasing the level of customer service, so it is necessary to develop appropriate changes in current assessment methods.
The thesis consists of three chapters. The first chapter examines personnel assessment methods and definitions, goals and describes uses and weaknesses. The second and third chapter examines the existing personnel assessment methods and analyzes the evaluation indicators and problems of appraisal systems. In conclusion, the author has summarized the conclusions and proposals for development of personnel assessment methods in company “DT Mobile”.
The thesis work consists of 64 pages, three chapters, 16 images, has been added two attachments
Cereolorum historia eorumque usus chirurgicus <dt.>
No full textAutopsie nach Ex. der ULB Sachsen-AnhaltVorlageform des Erscheinungsvermerks: Gießen 1796. bei Georg Friedrich Heyer
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