650 research outputs found
HERP Insertion Mixture v1
No full textThis is part of the HERP protocol, from: Alexander WG, Doering DT,andHittinger CT (2014) High-Efficiency Genome Editing and Allele Replacement in Prototrophic and Wild Strains of Saccharomyces.Genetics 198:859-866;doi:10.1534/genetics.114.170118 </p
Yeast Extract?Peptone?Glycerol +Antifolates (YPGly+ AF) Media v1
No full textThis recipe is from: Alexander WG, Doering DT,andHittinger CT (2014) High-Efficiency Genome Editing and Allele Replacement in Prototrophic and Wild Strains of Saccharomyces.Genetics 198:859-866;doi:10.1534/genetics.114.170118 See the full protocol for step-by-step YPGly+ AF preparation instructions. </p
HERP: Haploid Engineering and Replacement Protocol for Saccharomyces v1
No full textThis protocol is from: Alexander WG, Doering DT,andHittinger CT (2014) High-Efficiency Genome Editing and Allele Replacement in Prototrophic and Wild Strains of Saccharomyces.Genetics198:859-866;doi:10.1534/genetics.114.170118 Please see the full manuscript for additional details. The purpose of this document is to provide you with an easy‐to‐follow guide to using the HERP cassettes. We will go through the preparation of the selection and counterselection media, the culturing and transformation for insertion of the HERP cassettes, and counterseletive replacement of the HERP cassettes. </p
Reintegration and Transition Following Active Duty: Identifying the Challenges for Veterans Returning From Deployment in Afghanistan and Iraq
No full textAbstract
Date Presented 4/1/2017
A thematic synthesis of 65 articles on veteran transitions following deployment revealed four themes to explain the dynamic veteran population and associated challenges with transition back into civilian life. Results indicate how practitioners can play a larger role in the reintegration process.
Primary Author and Speaker: Beth Ann Walker
Additional Authors and Speakers: Jessica Bramstedt, Kathryn J. Cleary, Sara T. Greer, Drew A. Teague</jats:p
The IPHAS catalogue of H alpha emission-line sources in the northern Galactic plane
No full textWe present a catalogue of point-source H alpha emission-line objects selected from the INT/WFC Photometric Ha Survey (IPHAS) of the northern Galactic plane. The catalogue covers the magnitude range 13 <= r' <= 19.5 and includes Northern hemisphere sources in the Galactic latitude range -5 degrees < b < 5 degrees. It is derived from similar to 1500 deg(2) worth of imaging data, which represents 80 per cent of the final IPHAS survey area. The electronic version of the catalogue will be updated once the full survey data become available. In total, the present catalogue contains 4853 point sources that exhibit strong photometric evidence for Ha emission. We have so far analysed spectra for similar to 300 of these sources, confirming more than 95 per cent of them as genuine emission-line stars. A wide range of stellar populations are represented in the catalogue, including early-type emission-line stars, active late-type stars, interacting binaries, young stellar objects and compact nebulae.
The spatial distribution of catalogue objects shows overdensities near sites of recent or current star formation, as well as possible evidence for the warp of the Galactic plane. Photometrically, the incidence of Ha emission is bimodally distributed in (r' - i'). The blue peak is made up mostly of early-type emission-line stars, whereas the red peak may signal an increasing contribution from other objects, such as young/active low-mass stars. We have cross-matched our H alpha-excess catalogue against the emission-line star catalogue of Kohoutek & Wehmeyer, as well as against sources in SIMBAD. We find that fewer than 10 per cent of our sources can be matched to known objects of any type. Thus IPHAS is uncovering an order of magnitude more faint (r' > 13) emission-line objects than were previously known in the Milky Way
Long-term wind-driven X-ray spectral variability of NGC 1365 with Swift
No full textWe present long-term (months–years) X-ray spectral variability of the Seyfert 1.8 galaxy NGC 1365 as observed by Swift, which provides well-sampled observations over a much longer time-scale (six years) and a much larger flux range than is afforded by other observatories. At very low luminosities, the spectrum is very soft, becoming rapidly harder as the luminosity increases and then, above a particular luminosity, softening again. At a given flux level, the scatter in hardness ratio is not very large, meaning that the spectral shape is largely determined by the luminosity. The spectra were therefore summed in luminosity bins and fitted with a variety of models. The best-fitting model consists of two power laws, one unabsorbed and another, more luminous, which is absorbed. In this model, we find a range of intrinsic 0.5–10.0 keV luminosities of approximately 1.1–3.5 erg s?1, and a very large range of absorbing columns, of approximately 1022–1024 cm?2. Interestingly, we find that the absorbing column decreases with increasing luminosity, but that this result is not due to changes in ionization. We suggest that these observations might be interpreted in terms of a wind model in which the launch radius varies as a function of ionizing flux and disc temperature and therefore moves out with increasing accretion rate, i.e. increasing X-ray luminosity. Thus, depending on the inclination angle of the disc relative to the observer, the absorbing column may decrease as the accretion rate goes up. The weaker, unabsorbed, component may be a scattered component from the wind
The social cost of atmospheric release
No full textThe author presents a multi-impact economic valuation framework called the Social Cost of Atmospheric Release (SCAR) that extends the Social Cost of Carbon (SCC) used previously for carbon dioxide (CO2) to a broader range of pollutants and impacts. Values consistently incorporate health and agricultural impacts of air quality along with climate damages. The latter include damages associated with aerosol-induced hydrologic cycle changes that lead to net climate benefits when reducing cooling aerosols. Evaluating a 1% reduction in current global emissions, benefits with a high discount rate are greatest for reductions of sulfur dioxide (SO2), followed by co-emitted products of incomplete combustion (PIC) and then CO2 and methane. With a low discount rate, benefits are greatest for CO2 reductions, and are nearly equal to the total from SO2, PIC and methane. These results suggest that efforts to mitigate atmosphere-related environmental damages should target a broad set of emissions including CO2, methane and aerosols. Illustrative calculations indicate environmental damages are 0.73±0.34 per gallon of gasoline ($1.20±0.70 per gallon for diesel). These results suggest that total atmosphere-related environmental damages plus generation costs are greater for coal-fired power than other sources, and damages associated with gasoline vehicles exceed those for electric vehicles
Fibroblasts derived from oesophageal adenocarcinoma differ in DNA methylation profile from normal oesophageal fibroblasts
No full textOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.Oesophageal adenocarcinoma (OAC) is increasing in incidence and has a poor prognosis. Tumour derived fibroblasts (TDFs) differ functionally from normal fibroblasts (NDFs), and play a pivotal role in cancer. Many of the differences persist through subculture. We measured the DNA methylation profiles of 10 TDFs from OAC with 12 NDF from normal oesophageal mucosa using Infinium HumanMethylation450 Beadchips and found they differed in multidimensional scaling analysis. We identified 4,856 differentially methylated CpGs (DMCs, adjusted p 0.15), of which 3,243 (66.8%) were hypomethylated in TDFs compared to NDFs. Hypermethylated DMCs were enriched at transcription start sites (TSSs) and in CpG islands, and depleted in transcriptional enhancers. Gene ontology analysis of genes with DMCs at TSSs revealed an enrichment of genes involved in development, morphogenesis, migration, adhesion, regulation of processes and response to stimuli. Alpha-smooth muscle actin (α-SMA) is a marker of activated fibroblasts and a poor prognostic indicator in OAC. Hypomethylated DMCs were observed at the TSS of transcript variant 2 of α-SMA, which correlated with an increase in α-SMA protein expression. These data suggest that DNA methylation may contribute to the maintenance of the TDF phenotype
Genuine and simulated suicide notes: An analysis of content
No full textThe present study examined genuine and simulated suicide notes aiming to identify the measures of content that best differentiate between the two. Thirty-three genuine and thirty-three simulated suicide notes were content-analysed and data subjected to smallest space analysis (SSA), a Multidimensional Scaling Procedure. The core of all suicide notes was discovered to be constructed with the use of three variables: expressions of love, positive construction of partner and apologies. Furthermore, four different genuine suicide note themes (‘planned escape’, ‘negative affect and self-mitigation’, ‘positive affect and failed relationship’, ‘lack of self-acceptance’) and three simulated suicide note themes (‘escape’, ‘positive affect and self-blame’, ‘purposeless life’) were identified revealing that authentic suicide note themes were more internally consistent and clearer to interpret
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