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Two-photon fluorescence excitation within a light sheet based microscopy architecture
No full textLight-sheet microscopy, such as ultramicroscopy, single plane illumination microscopy (SPIM) [1] and digital scanned laser microscopy (DSLM) [2], represents a useful tool for biological investigations of thick samples. Such techniques have been found particularly useful in developmental biology applications since they provide the capability to perform fast imaging of living samples reducing photobleaching effects. The high signal to noise ratio and the intrinsic optical sectioning capability provided by SPIM suggest this technique as the best choice for imaging of thick scattering samples. Nevertheless, imaging in depth of large samples suffers from a decreasing in the image quality due to scattering effects. Two photon excitation microscopy [3] became a popular tool to perform imaging in turbid media since it improves the penetration depth capability and it reduces the image quality degradation due to scattering [4] and light matter interactions. Therefore, two photon excitation within the light sheet illumination scheme has been exploited in order to reduce scattering effects due to light-sample interactions. In this work two photon excitation imaging in SPIM scheme has been performed in order to achieve an improvement in the penetration depth while imaging living biological samples. © 2011 SPIE
Two-photon fluorescence excitation within a light sheet based microscopy architecture
No full textLight-sheet microscopy, such as ultramicroscopy, single plane illumination microscopy (SPIM) [1] and digital scanned laser microscopy (DSLM) [2], represents a useful tool for biological investigations of thick samples. Such techniques have been found particularly useful in developmental biology applications since they provide the capability to perform fast imaging of living samples reducing photobleaching effects. The high signal to noise ratio and the intrinsic optical sectioning capability provided by SPIM suggest this technique as the best choice for imaging of thick scattering samples. Nevertheless, imaging in depth of large samples suffers from a decreasing in the image quality due to scattering effects. Two photon excitation microscopy [3] became a popular tool to perform imaging in turbid media since it improves the penetration depth capability and it reduces the image quality degradation due to scattering [4] and light matter interactions. Therefore, two photon excitation within the light sheet illumination scheme has been exploited in order to reduce scattering effects due to light-sample interactions. In this work two photon excitation imaging in SPIM scheme has been performed in order to achieve an improvement in the penetration depth while imaging living biological samples
Motility flow and growth-cone navigation analysis during in vitro neuronal development by long-term bright-field imaging.
No full textGoing Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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