1,721,003 research outputs found
Combining RNAi and in vivo confocal microscopy analysis of the photoconvertible fluorescent protein Dendra2 to study a DNA repair protein
No full textClinical approaches for tumor treatment often rely on combination therapy where a DNA damaging agent is used in combination with a DNA repair protein inhibitor. For this reason, great efforts have been made during the last decade to identify inhibitors of DNA repair proteins or, alternatively, small molecules that specifically alter protein stability or trafficking. Unfortunately, when studying these drug candidates, classical biochemical approaches are prone to artifacts. The apurinic/apyrimidinic endonuclease (APE1) protein is an essential component of the base excision repair (BER) pathway that is responsible for repairing DNA damage caused by oxidative and alkylating agents. In this work, we combined conditional gene expression knockdown of APE1 protein by RNA interference (RNAi) technology with re-expression of an ectopic recombinant form of APE1 fused with the photoconvertible fluorescent protein (PCFP) Dendra2. Dendra2 did not alter the subcellular localization or endonuclease activity of APE1. We calculated APE1 half-life and compared these results with the classical biochemical approach, which is based on cycloheximide (CHX) treatment. In conclusion, we combined RNAi and in vivo confocal microscopy to study a DNA repair protein demonstrating the feasibility and the advantage of this approach for the study of the cellular dynamic of a DNA repair protein
Troponina e infarto periprocedurale: filo di Arianna o folle volo di Icaro?
Full text linkPercutaneous coronary interventions cause frequently an increase in myocardial necrosis markers. Is troponin elevation after percutaneous coronary intervention a predictor of events at follow-up or a consequence of the procedure with no cause-effect relationship with prognosis? The debate is still ope
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Troponin levels after elective percutaneous coronay intervention: outcome and follow-up
No full textTroponin is a diagnostic factor for cardiovascular disease for its specificity and myocardial tissue high sensitivy
Optimized design of high power density EMI filters for power electronic converters
No full textNowadays, power density of power converters and related EMI filters is gaining more and more attention. This severely impacts on the design constraints in several application domains. A conventional design of the EMI filter does not guarantee the selection of components/configuration leading to the best power density. For this reason, an optimized design procedure of discrete EMI filters in terms of power density is proposed in this paper. It is based on a previously developed rule-based design procedure, introducing here additional features to obtain a more effective optimization of EMI filter's power density. The proposed approach has been validated in terms of EMI filter's performance and size reduction, by a comparison between an EMI filter designed using the optimized procedure and a conventionally designed one, for two different case studies
Testosterone levels are directly related with erectile function and endothelial function in men with intermediate risk according to the Framingham score
No full textThe Role of Vitamin K in Bone Remodeling and Osteoporosis
No full textVitamin K is an essential fat soluble vitamin involved in the regulation of normal coagulation. However, growing
evidence highlights that this molecule appears to be also implicated in the regulation of other important
biological functions such as bone mineralization, calcium homeostasis, apoptosis, cell growth and signal
transduction. In particular, many studies have focused their attention on the protective effects of vitamin K on
bone tissue in the outlook of its use in the prevention and treatment of osteoporosis in post-menopausal women.
The objective of the present paper is to review data of the literature regarding the metabolic effects of Vitamin K
in bone tissue and its clinical role in the prevention and treatment of osteoporosis
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