1,720,966 research outputs found
Loco-regional immune default: the immunocompromised district in human and comparative dermatology
No full textLately, the innovative concept of an immunocompromised cutaneous district (ICD) has been introduced to explain why a previously injured cutaneous site may become in time a privileged location for the onset of opportunistic infections, tumors, and immune reactions. The injuring events capable of rendering a skin region a potential ICD are various, numerous, and most of the time identifiable by means of a careful clinical history. The reason that only a small minority of injured skin areas actually becomes ICDs, with subsequent opportunistic localization of a second and unrelated skin disorder, is presently unknown. The ICD is a conceptual entity that is not limited to human dermatology. It can also apply to veterinary medicine. Development of sarcomas at the injection site in cats after routine vaccination and, occasionally, administration of pharmaceutical products, as well as insertion of any foreign body, is a repeatedly documented event. Antigen load, persistent inflammation, and fibroblastic proliferation are thought to be important factors predisposing to the onset of fibrosarcoma in cats. Recently, it has been hypothesized that a local immunosuppression caused by inhalant glucocorticoids may have favored the development of regional demodicosis in cats. In our opinion, injection-site sarcomas and feline localized demodicosis can be considered examples of veterinary ICDs
Immunohistochemical and molecular investigation on the presence of Leishmania spp. in the skin of cats with head and neck ulcers
No full textBackground: Head and neck ulcers in cats are frequently due to allergic disorders but are also reported in feline leishmaniosis, a disease in which drugs used to treat allergy are contraindicated. Prevalence of Leishmania infection in cats from endemic regions is not negligible and parasitic DNA, but not amastigotes, was recently demonstrated in diseased and normal feline skin.
Objective: The aim of this study was to investigate, through immunohistochemical and molecular techniques, the presence of Leishmania in skin of cats with head and neck ulcers.
Methods: A retrospective histopathological, immunohistochemical and molecular study was conducted on paraffinembedded skin samples from 29 cats, most of them living in areas of Italy at potential risk for leishmaniosis, presenting
head and neck ulcerative dermatitis. Specimens were routinely stained with haematoxylin-eosin, immunostained using a polyclonal anti-Leishmania antibody and subjected to real-time Leishmania qPCR, including an internal reference. Along with other historical and clinical data, living conditions and sampling season were recorded.
Results: All specimens showed perivascular-to-interstitial, predominantly mastocytic, and to a lesser extent eosinophilicneutrophilic inflammatory infiltrate, with moderate-numerous macrophages in 19 samples. Immunostaining and PCR were all Leishmania-negative. Eighteen cats lived in Central-Southern Italy, 23 had outdoor access and 15 were sampled in Spring-Summer.
Conclusion: Despite lifestyle potentially associated with infection, Leishmania was not detected in skin lesions and parasites didn’t seem to play a contributory role to head and neck ulcers in studied cats
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
A retrospective histopathological, immunohistochemical and molecular study of the presence of Leishmania spp. in the skin of cats with head and neck ulcerative dermatitis
No full textBACKGROUND:
Head and neck ulcers in cats can arise from allergic and nonallergic disorders, including feline leishmaniosis (FeL). It is important to rule out this aetiological agent in regions that are endemic for canine leishmaniosis, because the drugs used to treat immune-mediated disorders of cats can be contraindicated in the setting of infection.
HYPOTHESIS/OBJECTIVES:
The aim of this study was to evaluate the skin of cats with ulcerative dermatitis of the head or neck for evidence of Leishmania infection using combined immunohistochemistry (IHC) and Polymerase Chain Reaction (PCR). An IHC for tissue histiocytes was also utilized because leishmaniosis may provoke a histiocytic inflammatory response.
ANIMALS:
Twenty seven cats with head and/or neck ulcers.
METHODS:
Skin biopsy specimens were examined for the presence of Leishmania spp. by routine histopathological evaluation and IHC using a polyclonal anti-Leishmania antibody, and by quantitative PCR (qPCR). The ionized calcium-binding adapter molecule-1 (IBA-1) antibody was used to immunolocalize histiocytes. Selected history and clinical data were recorded.
RESULTS:
All specimens showed a superficial mid-perivascular mixed inflammatory infiltrate. The presence of histiocytes was confirmed in 23 of 27 cases with the IBA-1 antibody. Immunohistochemistry and qPCR techniques confirmed the absence of Leishmania in all cases.
CONCLUSIONS AND CLINICAL IMPORTANCE:
Leishmania did not seem to play a role in the pathogenesis of feline ulcerative dermatitis of the head and neck in the subjects studied, despite a lifestyle potentially associated with infection. Histiocytic infiltration of tissue is not a specific marker for Leishmania infection in this population
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
University of Wisconsin solution and human saphenous vein graft preservation: preliminary anatomic report.
No full textEndothelial damage of human saphenous vein (HSV) during the preparation for bypass grafting could affect graft patency. Improving the preservation of HSV could provide a longer and better patency of coronary artery bypass grafts. An electron microscopic comparative analysis of the effects of three different preservatives on HSV morphology was carried on in order to determine the best method to prevent or minimize possible endothelial damage. Distal segments of HSVs were harvested from 15 patients with a "no-touch" technique. Each segment was divided into seven specimens after a low pressure distension with saline solution. The first of them was fixed immediately after harvesting with 2.5% glutaraldehyde solution, for basal evaluation. Three were fixed after 30 min and three after 5 h preservation at 4 degrees C in a) autologous, oxygenated, and heparinized blood (AOHB), b) heparinized saline solution with papaverine (HSSP) and c) University of Wisconsin solution (UWS). The specimens preserved in AOHB showed marked endothelial cell detachment and endothelial cell loss after both preservation times (30 min and 5 h). The specimens preserved in HSSP for 30 min showed no alteration in endothelium in 12 cases, while a partial endothelial detachment with intracellular edema was present in the last 3 cases; all 5 h specimens showed only a few remnants of endothelial cells. The specimens preserved in UWS for 30 min showed a morphology comparable to specimens preserved in the saline solution for the same time; in the 5 h specimens a well preserved endothelium was found in 11 cases and partial endothelial detachment and subendothelial edema in the other 4.(ABSTRACT TRUNCATED AT 250 WORDS
Metabolic syndrome in primary aldosteronism and essential hypertension: relationship to adiponectin gene variants.
No full textUniversity of Wisconsin solution and human saphenous vein graft preservation: preliminary anatomic report
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