1,720,962 research outputs found
Pharmacognostic approach to evaluate the micromorphological, phytochemical and biological features of Citrus lumia seeds
No full textThis study evaluated the micro-morphology as well as the chemical and biological features of Citrus lumia seeds. The cream-colored pyriform seed showed a woody coat covered by a thick layer of mucilage and an embryo with two large cotyledons rich in oil bodies. Hydroxycinnamic acid glycosides and flavonoids are the most abundant compounds in methanol and ethyl acetate extracts (ME and EAE), respectively. Conversely, fatty acids and alpha-tocopherol represent the main bioactive compounds in the hexane extract (HE). ME showed the most promising antioxidant and anti-inflammatory activities already in cell-free assays. These results were confirmed by experiments carried out on human primary cells. Indeed, ME showed the best inhibitory activity against heatinduced haemolysis and ROS formation in erythrocytes. Moreover, the same order of potency (ME > EAE > HE) was observed also on peripheral blood mononuclear cells, in which the seed extracts were able to decrease TNF-alpha and IL-6 release after LPS-induced infl
In vitro intestinal transport and anti-inflammatory properties of ideain across Caco-2 transwell model
No full textThe aim of the study was to investigate the absorption and transport mechanisms as well as the anti-inflammatory
properties of ideain on Caco-2 transwell model.
A concentration and time-dependent bidirectional transport was highlighted; despite this, a clear saturation
of the transepithelial absorption in the A-B direction was observed at ideain concentration > 10 μM, suggesting
an involvement of membrane transporters.
Comparing Papp and PDR values of ideain (10 μM) to reference drugs with a low to a high apparent permeability,
it is possible to predict a low in vivo absorption, with a transport efficiency of 1.03%. Co-treatments
with several EDTA-Na2 concentrations (1–5 mM) and P-gp inhibition studies with verapamil 100 μM ruled out a
passive diffusion of this molecule as well the possibility that P-gp could affect ideain absorption. Inhibition
studies using 2 mM phloridzin (SGLT1 inhibitor) and 2 mM phloretin (GLUT2 inhibitor), showed a clear SGLT1
and GLUT2 involvement in the ideain absorption, with SGLT1, which plays the pivotal role.
Finally, preliminary anti-inflammatory studies showed that ideain is able to modulate, at a pharmanutritional
dose, and with a comparable activity in respect to the reference drug dexamethasone (10 μM), the LPS-induced
inflammation in Caco-2 transwell model, which makes it a potentially useful molecule for nutraceutical purpos
Antioxidant and cytoprotective activities of an ancient Mediterranean citrus (Citrus lumia Risso) albedo extract: Microscopic observations and polyphenol characterization
No full textCitrus fruits are a rich source of bio-functional compounds with various and well-proven health properties. We focused our attention on an ancient Mediterranean species, Citrus lumia Risso. The aim of this work was to investigate the polyphenol content and biological activities of C. lumia albedo extract by cell-free and cell-based assays. Fifteen polyphenols were quantified by LC-DAD-FLD analysis (flavonoids 89.34% and phenolic acids 10.66%) with eriocitrin and hesperidin as major components (52.81% and 31.31%, respectively). These results were corroborated also by micromorphological observations, which showed clusters of needle-shaped crystals of hesperidin highlighted by TBO staining. C. lumia albedo extract revealed marked antioxidant and free radical scavenging properties, and a significant cytoprotective activity on t-BOOH-treated lymphocytes. Results indicate that C. lumia albedo extract could be exploited as an antioxidant source, suggesting the use of albedo, currently considered a by-product, as a valuable raw material for nutraceutical employments
Antioxidant and cytoprotective activities of an ancient Mediterranean citrus (Citrus lumia Risso) albedo extract: Microscopic observations and polyphenol characterization
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Il problema dell'errata identificazione tra piante selvatiche commestibili e velenose: reports dall'area mediterranea
No full textgenus: From ancient fruits to new hybrids
No full textCitrus fruits are among the most ancient known and have always attracted the interest
of humans, from both a nutritional and a health point of view. Even though there
is substantial scientific literature regarding the Citrus genus and associated isolated
bioactive compounds, there are still few studies on the botanical features, the phytochemical
profiles, and the biological activities for some ancient Citrus fruits as well as
for new hybrids that have become part of the internationalmarket.We have witnessed
both the rediscovery and revaluation of some cultivated ancient fruits in some limited
areas and the introduction of new hybrids destined to the food, ornamental, and food
design market. This is the first review that sheds light on the botanical and chemical features
aswell as on the biological properties of Citrus fruits that are under-investigated,
localizing them in an international context. Themanuscript focuses in particular on five
under-investigated species: Citrus australasica F. Muell. (Finger lime), Citrus medica L.
var. sarcodactylis Swingle (Buddha’s hand), Citrus junos Sieb ex Tan. (Yuzu), Citrus limon
(L.) Osbeck var. pompia Camarda (Pompia), and Citrus lumia Risso (Lumia)
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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