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First report of fruit tree phytoplasmas and their psyllid vectors in Bosnia and Herzegovina
First report of fruit tree phytoplasmas and their psyllids vectors in Bosnia and Herzegovina
Molecular characterisation of bois noir phytoplasmas from Bosnia and Herzegovina
Bois noir (BN) phytoplasmas were associated with grapevine yellows diseases in Bosnia and Herzegovina (Delic and Lolic, 2010). The disease is widely to moderate distributed in vineyards with both domestic and imported grapevine cultivars. Molecular characterisation of selected strains of BN phytoplasmas detected in heavily BN-infected vineyards in was done to verify the presence of genetic variability. Thirty-five DNA samples of grapevines that were shown to be positive by PCR assays on the 16S rDNA were selected for the study. Polymorphisms were studied in the 16S rDNA, Tuf and ribosomal protein genes of the selected samples. Tuf1f/r, TufAYf/r, and TufINT1f/TufINT4r primer pairs were used in nested PCR for amplification of Tuf genes; RFLP analyses with HpaII showed the presence of the tuf type-b (VK-II) in all the tested samples.
The BN infected samples were further amplified with rp/STOL primers and PCR products were digested with TaiI and SspI restriction enzymes. Finally 16S rDNA genes were amplified with P1/P7 primers in direct PCR and with R16F2n/R16R2 primers in nested PCR assays: both types of amplicons obtained were restricted with MboII and RsaI, MboII, Hpy188I enzymes, respectively.
Restriction profiles obtained after RFLP analyses on 16S rDNA and ribosomal protein amplicons weren't uniform. Some samples showed profiles comparable with some of those recently published (Contaldo et al., 2009), while some of the profiles of other samples were totally different from any of those reported in literature. Therefore, polymorphism of these regions should be further studied to better understand BN epidemiology in Bosnia and Herzegovina
Going Beyond Counting First Authors in Author Co-citation Analysis
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Grapevine yellows in Bosnia and Herzegovina: surveys to identify phytoplasmas in grapevine, weeds and insect vectors
Presence and diffusion of grapevine yellows phytoplasmas was investigated in 2008 and 2010 in twelve vineyards located in two viticultural areas of Srpska region of Bosnia and Herzegovina. From the same vineyards, grapevine samples from different culti-vars and weeds were collected for molecular analyses. In addition, some potential insect vectors were collected in the vineyards of these regions. ‘Bois noir’ phytoplasma presence was confirmed to be associated with grapevine yellows, while in one Clematis vitalba sample phytoplasma from 16SrV-C subgroup was identified. RFLP analysis of the tuf gene indicated the presence of the tuf type-b of stolbur phytoplasma in the ‘bois noir’-infected samples. From collected hemipteran insects Dictyophara europaea and Reptalus cuspidatus were identified. Molecular analyses didn't show phytoplasma presence in the tested insect samples of these species
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