3,772 research outputs found
Le (ri)scritture del trauma: Giani Stuparich oltre la «Trilogia della guerra» (1916-1941)
No full textIl contributo ripercorre l'elaborazione dell'esperienza bellica negli scritti di Giani Stuparich, proponendo un percorso che dagli scritti privati di prigionia giunge fino alla composizione del romanzo 'Ritorneranno': la sopravvivenza del trauma collettivo, cui si aggiunge il lutto privato per la morte del fratello, rappresenta per oltre vent'anni il motore della scrittura stupariciana - tesa alla ricerca della forma più adatta per raccontare la realtà e il significato profondo della Grande guerra
Glutathione S-transferases in Festuca arundinacea : Identification, characterization and inducibility by safener benoxacor
No full textOver recent years it has emerged how certain no crop-species can be employed in phytoremediating contaminated soils or preventing
herbicide pollution; in this contest Festuca arundinacea was investigated. Shoots of Festuca were submitted to fast protein liquid chromatography
in order to identify their glutathione S-transferases (GST; EC 2.5.1.18), by a combination of anionic, affinity and RP-HPLC
chromatography. The chromatographic procedure revealed satisfactory yield and four GSTs were identified: they were named FaGST I,
FaGST II, FaGST III and FaGST IV. Among these, significant differences were observed in the chromatographic behaviours, structure,
activity toward a ‘‘model’’ substrate, 1-chloro-2,4-dinitrobenzene, and responsiveness to the herbicide safener benoxacor. FaGST I
showed the highest activity toward the above substrate, and this activity was up-regulated by the herbicide safener. Therefore, FaGST
I was purified till homogeneity and was determined to be an heterodimer consisting of two subunits of 28.0 and 27.2 kDa. Each subunit
of FaGST I was further characterized by means of LC–ESI–MS/MS and immunoblotting analysis, which revealed that both the subunits
belong to the tau subclass
Analytical methods for the determination of pretilachlor and fenclorim in soil and rice plants
No full textImazamox in maize: uptake, persistence and interference on protein and carbohydrate formation
No full textThe relationship between obsessive-compulsive personality and obsessive-compulsive disorder: data obtained by the Personality Disorder Examination
No full textEffect of pretilachlor and fenclorim on carbohydrate and protein formation in relation to their persistence in rice
No full textPersistence and detoxification of Pretilachlor and Fenclorim in rice (Oryza sativa)
No full textThe contents and persistence of pretilachlor (P) and of the safener
fenclorim (F) in rice treated with P, F and their mixture were investigated
in relation to the expression of glutathione-S-transferase (GST) activity
in response to treatments. F failed to reduce the accumulation of P, however
it was able to reduce its persistence in rice shoots. On the other hand,
while P did not reduced F persistence, it determined a greater than two-fold
F accumulation in plants. Therefore, a detoxification mechanism common to
the two compounds but favourable to P was hypothesised. This mechanism
consists in an activation of GST activity toward P as substrate [GST(P)]
in response to all treatments. The activation levels were found to be
proportional to the amount of persistent P and F, and to be additive
following the P-F mixture treatment. The kinetic parameters V
and K of GST(P) were increased and decreased, respectively, in rice shoots
treated with P, F and particularly with P-F mixture. Therefore, a defence
mechanism toward P, consisting in an increased expression of GST(P) in rice
plant, both as the global amount of the enzyme in the protein “pull” and the
induction of the enzyme isoforms more sensitive to P, in response to all
treatments, especially the P-F mixture treatment, is confirmed. This finding
is consistent with the observed effect of F on P persistence and detoxification
in the rice shoots.Persistance et détoxication du pretilachlore et du fenclorim dans
le riz (Oryza sativa). On a étudié la teneur et la persistence du
pretilachlore (P) et du fenclorim (F), un antidote, dans le riz traité
avec P, F et un mélange des deux par référence à l'activité de la
glutathion-S-transférase (GST) en réponse aux traitements en question.
F n'a pas réussi à réduire l'accumulation de P, mais a pu réduire sa
persistance dans les pousses de riz. D'autre part, P n'a pas réduit
la persistance de F, mais a déterminé une accumulation plus que double
de F dans les plantes. Donc a-t-on soulevé l'hypothèse d'un mécanisme
de détoxication commun aux deux composés, mais favorable à P.
Ce mécanisme consiste en une activation de l'activité GST à l'égard
de P comme substrat [GST(P)] en réponse à tous les traitements. On a
relevé que les niveaux d'activation étaient proportionnels à la quantité
de P et F persistants, et additifs dans le cas du traitement avec le
mélange P-F. Les paramètres cinétiques V et K
de [GST(P)] ont été respectivement augmentés et diminués dans les pousses
de riz traitées avec P, F et particulièrement avec le mélange P-F. On a eu donc
confirmation d'un mécanisme de défense à l'égard de P, consistant
en une expression accrue de [GST(P)] dans la plante de riz, en ce qui
concerne tant la quantité globale de l'enzyme dans le “ pull ” protéique
que l'induction des isoformes enzymatiques plus sensibles à P, en réponse
à tous les traitements et surtout au traitement à base de mélange P-F.
Ce résultat concorde avec ce qui a été observé quant à l'effet de F sur
la persistance et la détoxication de P dans les pousses de riz
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