1,720,961 research outputs found
Silencing of ZRF1 impedes survival of estrogen receptor positive MCF-7 cells and potentiates the effect of curcumin
No full textThe role and clinical implication of ZRF1 in breast cancer are poorly understood. So this study is aimed to explore the role of ZRF1 in breast cancer progression. With this context, we first assessed its expression pattern in FFPE primary and metastasis breast tissue samples as well as from publicly available databases. Moreover, we also explored the survival status of patients from the publicly available database and interestingly discover that high expression of ZRF1 decreases the survival of estrogen-positive breast cancer patients more than estrogen-negative status patients. In the perspective of this, we evaluated the role ZRF1 in MCF-7 breast cancer cells and found that it's silencing by knockdown results in decreased cell proliferation as well as cell viability. Results also show that expression of ZRF1 is down regulated in the presence of estrogen-depleted conditions but independent of RAS/MEK as well as AKT axes. Moreover, the decrease in viability of MCF-7 cells was accompanied by induction of apoptosis and DNA damage, well-marked with upregulation of cleaved PARP and downregulation of BCL2 and H2AUbK119 levels. Furthermore, we also explored that knockdown of ZRF1 sensitises the effect of curcumin, observed with decrease in cell viability and dropping of IC50 value from 25 to 15 mu M. This investigation thus shed a new light on the role on ZRF1 in breast cancer cells and hence can be exploited to design better therapeutic intervention.NIT-Rourkela; DST, Government of Indi
Epigenetic Signatures of Genes and Their Correlations with Various Signaling Pathways During Tumorigenesis
No full textDuring the past 15 years the epigenetic regulation of gene has been studied extensively. The genesis of cancer and epigenetic regulation of genes are deeply interconnected. DNA methylation, histone tail modification, nucleosome remodelling, and non-coding RNA regulate many biological processes that are elementary to the cancer development. This thesis evaluates the expression profile and epigenetic regulation (especially, DNA methylation and histone H3 modifications) of various genes, such as caveolin 1 (CAV1), clusterin (CLU), beta 1 integrin (β1 integrin), histone H3.3 and chromatin modifying enzymes like DNA methyltransferases (DNMTs), histone methyltransferases (HMTs) and histone acetyltransferases (HATs) in colon and breast cancers. Additionally, lipid raft, RAS/MEK/ERK and FAK signaling pathway mediated regulation of histone modifications in colon cancer is also examined. mRNA and protein level analysis of respective gene products in cancer tissue samples and cell lines demonstrated that CAV1 is expressed in a stage-specific manner whereas nuclear CLU (nCLU) is down-regulated; whereas, secretory CLU (sCLU), β1 integrin and H3.3 genes are up-regulated in both the cancers. Inhibition of cell growth in breast and colon cancer cell lines after treatment with epigenetic modulators is associated with up-regulation of CAV1 and nCLU; ultimately resulting in down-regulation of β1 integrin. On investigating the epigenetic regulatory mechanisms of CAV1 gene, it is observed that histone modifications (H3K4me3, H3K9me3, H3K9AcS10p) predominantly regulate CAV1 expression in both cancers whereas promoter DNA methylation is partially responsible in case of only breast cancer. Moreover, expression of CLU is associated with global histone marks in case of breast cancer. In colon cancer, promoter H3K4me, H3K9me3 and H3K9AcS10p enrichment is the predominant regulator of CLU gene expression. Along with expression pattern and epigenetic regulation this thesis also evaluated the involvement of different signaling responsible for histone modifications. The modulation of global and gene specific histone marks in colon cancer is demonstrated to be regulated by lipid raft, RAS/MEK/ERK and FAK signaling pathways. These pathways influence global H3K4me3, H3K9me3, H3K9AcS10p levels and in turn differentially regulate gene specific expression, such as that of CAV1
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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