1,720,986 research outputs found
Photoelectron spectromicroscopy with synchrotron radiation: Applications to neurobiology
No full textWe show that photoelectron spectromicroscopy with synchrotron radiation has been successfully exported from materials science to the life sciences. The transfer is well beyond mere feasibility tests. Photoelectron spectromicroscopy is in fact used for real, long-term studies in neurobiology. Specific examples are discussed, first to see in practice some applications to microchemical analysis of physiologic elements, and then to discuss present and future instrumentation development issues. This last point is illustrated by briefly presenting the MEPHISTO project and the CERVIN project for the Swiss Light Source. (C) 1997 Elsevier Science B.V.LSELPRXCnr,ist struttura mat,i-00044 frascati,roma,italy. DeStasio, G, ECOLE POLYTECH FED LAUSANNE,PHB ECUBLENS,INST PHYS APPL,CH-1015 LAUSANNE,SWITZERLAND. ISI Document Delivery No.: XH46
Photoemission Spectromicroscopy of Neurons
No full textWe present photoemission spectromicroscopy results on biological specimens. The data, obtained with x-ray secondary (photoelectron) emission microscopy, study individual elements in micrometer-size portions of neuron networks.LSELPRXUniv wisconsin,dept phys,milwaukee,wi 53211. univ wisconsin,ctr synchrotron radiat,madison,wi 53706. cnr,inst neurobiol,i-00100 rome,italy. ecole polytech fed lausanne,ph ecublens,inst phys appl,ch-1015 lausanne,switzerland. Destasio, g, cnr,ist struttura mat,via enrico fermi 38,frascati,italy.ISI Document Delivery No.: KY13
Low-Energy-Electron Microscopy (Leem) Imaging of a Neuron Network
No full textThe feasibility of using the LEEM technique in neurobiology was tested with experiments on a rat cerebellar granule cell culture. A series of good-quality images, showing fine details such as axons and dendrites of the neuron network, demonstrate that the test was successful.LSELPRXCnr,ist struttura mat,rome,italy. portland state univ,dept phys,portland,or 97207. cnr,ist neurobiol,i-00137 rome,italy. Destasio, g, ecole polytech fed lausanne,ph ecublens,inst phys appl,ch-1015 lausanne,switzerland.ISI Document Delivery No.: QN42
Scanning Tunneling Microscopy of Deoxyribonucleic-Acid During Replication
No full textScanning tunneling microscopy was used to produce topographic images of uncoated and unlabeled deoxyribonucleic acid (DNA) in air, on a graphite substrate. The images show for the first time a DNA molecule that had been isolated while it was replicating.LSELPRXUniv wisconsin,dept phys,madison,wi 53706. univ wisconsin,ctr synchrotron radiat,madison,wi 53706. ist neurobiol consiglio nazl ricerche,i-00137 rome,italy. infn,nazl frascati lab,i-00044 frascati,italy. univ wisconsin hosp,madison,wi 53706. Destasio, g, inst struttura mat consiglio nazl ricerche,via e fermi 38,i-00044 frascati,italy.ISI Document Delivery No.: FV99
Aluminum in Rat Cerebellar Primary Cultures - Glial-Cells and Gabaergic Neurons
No full textEXPERIMENTAL evidence of the preferential uptake of aluminium by GABAergic neurones and glial cells was provided by synchrotron spectromicroscopy studies. We observed rat cerebellar cultures enriched for GABAergic neurones or glial cells exposed to aluminium ions, detecting the presence and identifying the chemical status of aluminium on cell structures.LSELPRXEcole polytech fed lausanne,phb ecublens,inst phys appl,ch-1015 lausanne,switzerland. cnr,ist neurobiol,i-00137 rome,italy. univ wisconsin,dept phys,milwaukee,wi 53211. univ wisconsin,ctr synchrotron radiat,madison,wi 53706. Destasio, g, cnr,ist struttura mat,via enrico fermi 38,i-00044 frascati,italy.ISI Document Delivery No.: PL23
Aluminum in Rat Cerebellar Neural Cultures
No full textA SYSTEMATIC Microchemical analysis of unstained and uncoated neurone cultures was performed with synchrotron radiation photoemission spectromicroscopy after exposure to an aluminium solution. Clear evidence was found for localized aluminium uptake in a few cells. Their possible identification based on morphology is discussed.LSELPRXUniv wisconsin,dept phys,milwaukee,wi 53201. univ wisconsin,ctr synchrotron radiat,madison,wi 53706. ist neurobiol consiglio nazl ricerche,rome,italy. ecole polytech fed lausanne,inst phys appl,ch-1007 lausanne,switzerland. Destasio, g, cnr,ist struttura materia,via enrico fermi 38,frascati,italy.ISI Document Delivery No.: LW48
Scanning Photoelectron Microscopy with Undulator Radiation - a Successful Test on Uncoated Neurons
No full textLSELPRXUniv wisconsin,dept phys,madison,wi 53706. univ wisconsin,dept elect & comp engn,madison,wi 53706. univ wisconsin,ctr synchrotron radiat,madison,wi 53706. univ calif berkeley lawrence berkeley lab,ctr xray opt,berkeley,ca 94720. cnr,ist neurobiol,i-00100 rome,italy. Destasio, g, cnr,ist struttura mat,via e fermi 38,i-00044 frascati,italy.ISI Document Delivery No.: DZ53
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
X-Ray Secondary-Emission Microscopy (Xsem) of Neurons
No full textWe present the first X-ray secondary (photoelectron) emission microscopy (XSEM) pictures and video microimages of an uncoated and unstained neuron specimen. This novel kind of synchrotron radiation microscopy is suitable for local chemical analysis with a lateral resolution in the micron range. We explored the details of the neuron system, demonstrated chemical contrast by scanning the photon energy, studied in real time the photoelectron emitting properties of the specimen's components, and made preliminary tests of the radiation damage. These results significantly enhance the potential role of photoemission techniques in the life sciences and specifically in neurobiology.LSELPRXUniv wisconsin,dept phys,milwaukee,wi 53211. univ wisconsin,ctr synchrotron radiat,stoughton,wi 53589. cnr,ist neurobiol,i-00100 rome,italy. ecole polytech fed lausanne,inst phys appl,ch-1015 lausanne,switzerland. Destasio, g, cnr,ist struttura mat,via enrico fermi 38,i-00044 frascati,italy.ISI Document Delivery No.: JK28
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