1,721,020 research outputs found
15. De Santis (T). Sibaritide a ritroso nel tempo. Cosenza, 1960 ; en vente à la Libreria editrice L'Erma di Bretschneider, Rome
No full textLévêque Pierre. 15. De Santis (T). Sibaritide a ritroso nel tempo. Cosenza, 1960 ; en vente à la Libreria editrice L'Erma di Bretschneider, Rome. In: Revue des Études Grecques, tome 75, fascicule 354-355, Janvier-juin 1962. pp. 256-257
Ruolo dei peptidi oppioidi endogeni (EOP) come coregolatori ad azione locale della maturazione dell'ovocita e dell'embriogenesi in vitro negli equini
No full textSummary
In this study, we report the detection of the mu-opioid receptor in equine cumulus-oocyte complexes, both at the mRNA and the protein end product level. The effects of beta-endorphin and of the opioid antagonist naloxone (Nx) on the maturation rate (MR) and cytoskeletal organization (microtubules and microfila- ments) of oocytes recovered in different seasons of the year (spring transition, breeding season, autumnal transition and winter anestrous) and showing initial compact or expanded cumulus oophorus morphology have been examined by confocal laser scanning microscopy (CLSM). In parallel, modifications of intra- cellular calcium concentration ([ C a^ 2+ ] i ) in cumulus cells incubated in presence of Nx or beta-endorphin have been evaluated by microspectofluorimetry. Variable effects of Nx and beta-endorphin on MR, cytoskeletal organization and [ C a^ 2+ ] may be related to different levels of mu-opioid receptor expression or activation status that could underlay different meiotic competence of equine oocytes
Analisi della distribuzione mitocondriale di ovociti equini maturati in vitro in presenza di Naloxone
No full textMitochondrial distribution and activity in human mature oocytes: GnRH agonist vs antagonist for pituitary downregulation
No full textObjective: To analyze the effects of GnRH agonists versus antagonists on mitochondrial distribution and activity
in human mature oocytes.
Design: Randomized research experimental study.
Setting: Academic basic research laboratory and hospital-based fertility center.
Patient(s): Two hundred twenty-five supernumerary mature oocytes from 44 patients.
Intervention(s): Fluorescent staining and confocal laser scanning microscopy on oocytes after the use of either
GnRH agonist (group A) or GnRH antagonist (group B).
Main Outcome Measure(s): Oocyte mitochondrial distribution pattern and activity using MitoTracker Orange
CMTM Ros.
Result(s): More oocytes showing polarized mitochondrial distribution pattern were found in group A than in
group B (35% vs. 14%). In group B, hCG rather than GnRH agonist, for ovulation induction, resulted in more
oocytes showing heterogeneous (57% vs. 14%), in particular polarized (24% vs. 0) mitochondrial distribution.
In groups A and B, fluorescence intensity did not vary according to mitochondrial distribution pattern. However,
fluorescence intensity was higher in oocytes with polarized and large granules configurations in group B compared
to group A.
Conclusion(s): The GnRH agonist and antagonist may have different effects on oocyte mitochondrial distribution
pattern and activity. The GnRH antagonist may induce mitochondrial hyperactivity, which may be detrimental to
the oocyte. (Fertil Steril 2009;91:249–55. 2009 by American Society for Reproductive Medicine.
Analisi della distribuzione mitocondriale in ovocita bovini maturati in vitro. Analisys of mitochondrial distribution in in vitro matured bovine oocytes
No full textAnalisi della distribuzione mitocondriale di ovociti equini maturati in vitro in presenza di Naloxone. Analysis of mitochondrial distribution of equine. oocytes matured in vitro in presence of Naloxone
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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