49 research outputs found
Middle age has a significant impact on gene expression during skin wound healing in male mice
No full textThe vast majority of research on the impact of age on skin wound healing (WH) compares old animals to young ones. The middle age is often ignored in biogerontological research despite the fact that many functions that decline in an age-dependent manner have starting points in mid-life. With this in mind, we examined gene expression patterns during skin WH in late middle-aged versus young adult male mice, using the head and back punch models. The rationale behind this study was that the impact of age would first be detectable at the transcriptional level. We pinpointed several pathways which were over-activated in the middle-aged mice, both in the intact skin and during WH. Among them were various metabolic, immune-inflammatory and growth-promoting pathways. These transcriptional changes were much more pronounced in the head than in the back. In summary, the middle age has a significant impact on gene expression in intact and healing skin. It seems that the head punch model is more sensitive to the effect of age than the back model, and we suggest that it should be more widely applied in aging research on wound healing
Die Rolle des Kallikrein-Kinin-Systems bei der fokalen zerebralen Ischämie
No full textDer ischämische Schlaganfall ist eine der häufigsten Ursachen für Mortalität und Behinderungen in der westlichen Welt – entsprechend nimmt die gesundheitliche und volkswirtschaftliche Bedeutung dieser Erkrankung einen hohen Stellenwert ein.
Eine große Anzahl experimenteller und klinischer Studien befasste sich in den letzten Jahren mit verschiedenen Mechanismen zur Neuroprotektion, konnte aber die in der experimentellen Forschung erbrachten Ergebnisse in der Klinik nicht bestätigen.
Ziel dieser Arbeit war es daher, die therapeutische Wirkung der Blockade des Kallikrein-Kinin-Systems mittels Antagonisierung des Bradykinin B2-Rezeptors mit einem Nicht-Peptid Antagonisten in einem Tiermodell der temporären fokalen zerebralen Ischämie zu untersuchen und dabei seine prä- und postischämischen Effekte und möglichen Wirkmechanismen zu erforschen.
Unsere Ergebnisse bestätigen die durch B2-Rezeptor-Aktivierung vermittelte Mediatorfunktion des Kallikrein-Kinin-Systems bei der fokalen zerebralen Ischämie. Wir konnten erstmals nachweisen, dass die Blockade des B2-Rezeptors mit dem neu entwickelten Nicht-Peptid Antagonisten LF 16-0687 MS die Infarktbildung hemmt und die funktionelle neurologische Erholung nach temporärer fokaler zerebraler Ischämie bei Ratten verbessert, ohne Nebenwirkungen auf physiologische Kontrollparameter auszuüben.
Die dosisabhängige Wirkung von LF 16-0687 Ms konnte in unserem Modell der temporären fokalen zerebralen Ischämie bei der Ratte bestätigt werden.
Mit unseren Ergebnissen konnten wir quantitativ nachweisen, dass durch die B2-Rezeptor-Blockade mit LF 16-0687 Ms die postischämische Hirnschwellung nach fokaler zerebraler Ischämie bei Ratten gehemmt wird.
Die neuroprotektive Wirkung besteht auch bei Therapiebeginn nach Ischämie, was die weitere in vivo und in vitro Evaluierung des Antagonisten verdient.
Die Ergebnisse dieser Arbeit bilden die Grundlage für weitere Untersuchungen des Kallikrein-Kinin-Systems insbesondere der B1- und B2-Rezeptor-Interaktionen bei fokaler und globaler zerebraler Ischämie, bei Schädel-Hirn-Trauma und bei in vitro Untersuchungen
Dermal Replacements in General, Burn, and Plastic SurgeryTissue Engineering in Clinical Practice /
No full textVI, 227 p. 64 illus., 49 illus. in color.online r
Comparison of Meshed versus MEEK Micrografted Skin Expansion Rate: Claimed, Achieved, and Polled Results
No full textPendidikan Antropologi dan Pembangunan Indonesia
No full textThis article was written in response to a challenge put forth by two non-anthropologists over the role of anthropologists in Indonesian national development. The challenge was made by the late Dr. Y.B. Mangunwijaya (Kompas 24 January 1996) and Dr. Benjamin Lumenta (Kompas 29 January 1996). In fact, a response was given by Prof. Parsudi Suparlan (Kompas3 February 1996) and the author (Republika 2 May 1996). Also related to the matter is an article by Prof. S. Budhisantoso (Republika 24 May 1996).This article is an extension of the author's own article that appeared in Republika of May2, 1996. He finds that his ideas on the role of anthropologists in national development could not be covered adequately in the brief newspaper article, and requires an extended and serious discussion-even more so since the present articles touches upon the anthropological education system in Indonesia, specifically at the Department of Anthropology at the University of Indonesia. Thus, the article brings forth three main points, that is the role of anthropologists in Indonesia, the development of the anthropological education system in Indonesia, and Indonesian development
The surgical treatment of rhinophyma—Complete excision and single-step reconstruction by use of a collagen–elastin matrix and an autologous non-meshed split-thickness skin graft
No full textAbstractINTRODUCTIONRhinophyma is the most common clinical manifestation of phytamous rosacea. While conservative methods (e.g. topical or systemic antibiotics, retinoids) are effective for the treatment of acne rosacea, a surgical intervention is appropriate and required in established rhinophyma. A variety of surgical techniques to reduce proliferated tissue have been reported. However, a surgical “gold standard” for treating the distorting phymatous skin alterations has not yet been established.PRESENTATION OF CASEThis case report details a novel surgical approach: the combination of a bovine collagen–elastin with simultaneous autologous non-meshed split-thickness skin grafting.DISCUSSIONOur approach was based on the following considerations: deep excision facilitates complete removal of diseased tissue that ultimately reduces the risk of recurrence in contrast to commonly applied methods relying predominantly on superficial lesion removal with subsequent spontaneous re-epithelialization. The application of a dermal substitute to create a neodermis covered by split-thickness autologous skin grafting may serve as a functionally and aesthetically appropriate model without requiring the recruitment of donor sites for full-thickness skin grafts or even local flaps.CONCLUSIONThe combination of deep excision and single-step replacement of epidermal–dermal components may ultimately avoid the recurrence of rhinophyma and contribute to a full skin repair leading to satisfactory functional and aesthetic outcome
