76 research outputs found
Genetic Similarity Analysis in Lentil Using Cross-Genera Legume Sequence Tagged Microsatellite Site Markers
No full textMolecular markers have emerged as useful tools to assess the genetic diversity across crops. In lentil, molecular markers are limited. The objective of the study was to explore cross-genera transferability of sequence tagged microsatellite site (STMS) markers from related legumes and assess their utility in lentils. Thirty lentil (Lens culinaris Medik. subsp. culinaris) accessions were evaluated for genetic similarity analysis using cross-genera STMS markers. Thirty-nine STMS markers amplified 68 alleles with an average of 1.74 alleles per locus. Twenty lentil-specific STMS markers produced a total of 36 amplicons, of which 90% (18) markers were polymorphic. A maximum of four alleles were obtained with primers SSR13 and SSR19. Of 47 STMS markers from other legume genera, only 19 markers produced 32 scorable amplicons, and only 58% (11) of the amplified markers exhibited polymorphism. The polymorphism information content values observed with lentil specific markers ranged from 0.02 to 0.99, while for transferrable markers it ranged from 0.06 to 0.84. Maximum genetic similarity was observed between ‘NDL1’ and ‘LH84-8’ (0.942) and minimum between ‘PL234’ and ‘Precoz’ (0.709). The dendrogram based on Jaccard's similarity coefficients showed limited genetic variability among the cultivars included in the present study. A combination of lentil-specific and transferrable STMS markers was successfully used for identification of genetic similarity in lentil germplasm
Inheritance of Protruded Stigma in Black Gram [Vigna mungo (L.) Hepper]
No full textInheritance of protruded stigma in black gram [Vigna mungo (L.) Hepper] was investigated in the F(1), F(2), and F(3) generations of four crosses and their reciprocals involving a natural flower mutant with its parent genotype (IPU 99-167) and three other genotypes (PLU 710, Type 9, and Barabanki local). The outcrossing in the mutant was due to a flower mutant with protruded stigma and crumpled petals, thus presenting a physical condition for functional male sterility. The mutant plants appeared in the parent population at an average frequency of 3.05%. The F(1) plants showed normal flowers with normal pod and seed set. The segregation analysis of F(2) and F(3) populations indicated a single recessive gene conditioning protruded stigma and crumpled petals with pleiotropic effect. The gene symbol stg1stg1 is proposed for this mutation. Under natural conditions in the crossing block, 100% of the seeds obtained from the mutant plants were the result of cross pollination despite 93.6% pollen viability. However, the number of seeds produced by the mutant was 92% less than normal plants. Although the mutant genotype provides a mechanism for outcrossing, unless the poor seed set can be improved (e.g., by delayed planting), its use in exploiting heterosis and population improvement will be limited
A relatively light, highly bino-like dark matter in the -symmetric NMSSM and recent LHC searches
No full textA highly bino-like Dark Matter (DM), which is the Lightest Supersymmetric
Particle (LSP), could be motivated by the stringent upper bounds on the DM
direct detection rates. This is especially so when its mass is around or below
100 GeV for which such a bound tends to get most severe. Requiring not so large
a higgsino mass parameter, that would render the scenario reasonably natural,
prompts such a bino-like state to be relatively light. In the Minimal
Supersymmetric Standard Model (MSSM), in the absence of comparably light
scalars, such an excitation, if it has to be a thermal relic, is unable to meet
the stringent experimental upper bound on its abundance unless its
self-annihilation hits a funnel involving either the -boson or the Standard
Model (SM)-like Higgs boson. We demonstrate that, in such a realistic
situation, a highly bino-like DM of the popular -symmetric Next-to-Minimal
Supersymmetric Standard Model (NMSSM) is viable over an extended range of its
mass, from our targeted maximum in the vicinity of the mass of the top quark
down to about 30 GeV. This is facilitated by the presence of comparably light
singlet-like states that could serve as funnel (scalars) and/or coannihilating
(singlino) states even as the bino-like LSP receives a minimal (but optimal)
tempering triggered by suitably light higgsino states that, in the first place,
evade stringent lower bounds on their masses that can be derived from the Large
Hadron Collider (LHC) experiments only in the presence of a lighter
singlino-like state. An involved set of blind spot conditions is derived for
the DM direct detection rates by considering for the very first time the
augmented system of neutralinos comprising of the bino, the higgsinos and the
singlino which highlights the important roles played by the NMSSM parameters
and in delivering a richer phenomenology.Comment: 70 pages, 16 figures and 3 table
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No full textNot AvailableMorphologically normal and fertile transgenic chickpea plants have been regenerated through a standardized transformation protocols. This protocol is based on the infection of apical meristem explants (AME) with Agrobacterium strain EHA105. The stain, carrying pCAMBIA2301 vector contained β-glucuronidase (uidA) gene and neomycin phosphotransferase (nptII) genes. Different explants of chickpea and Agrobacterium specific conditions were standardized with the help of transient β-glucuronidase (uidA) gene expression to further optimize the transformation protocol. Pre-conditiong of the explants, vacuum infiltration and presence of acetosyringone significantly enhanced the frequency of gus expression. Positive transformants with nptII and gus genes were confirmed by PCR and histochemical gus analysis. An overall successful chickpea transformation frequency of 1.2 was achieved. This high efficiency and easy to use method may provide opportunities for the development of transgenic lines with different useful genes in chickpea in near future.Not Availabl
Development of EST derived microsatellite markers in chickpea and their validation in diversity analysis
No full text55-58Microsatellites are widely used as genetic
markers because they are co-dominant, multi-allelic, easily scorable and
highly polymorphic. In order to enhance availability of genomic resources, microsatellite
loci were identified from chickpea (Cicer
arietinum L.), the third most important grain legume in the world. A total
of 20 SSR markers were developed from EST clones of wilt resistant cultivar (JG
315) of chickpea. Chickpea varieties (15) were analyzed for genetic diversity
with these markers, which produced a total of 35 alleles with a mean of 1.5
alleles per primer. About 5 markers were polymorphic in the selected genotypes
and observed heterozygosity ranged from 0.12 to 0.87 with an average of 0.32.
These microsatellite markers will be useful in diversity analysis, mapping
agronomically important traits and marker assisted breeding in chickpea
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No full textNot AvailablePaucity of polymorphic molecular markers in pigeonpea, Cajanus cajan (L.) Millsp., has been a major limiting factor in application of molecular tools for its genetic improvement. As the development of microsatellite markers requires considerable time, expertise and research infrastructure, transfer of markers from other related genera offers an alternative option to increase the number of available markers. Since microsatellite sequences are conserved across Fabaceae taxa, transferability of 100 chickpea (Cicer arietinum L.)-specific SSR markers was studied in two genotypes each of five wild and one cultivated species of Cajanus. The results revealed a significant transferability (46%) of chickpea microsatellites to Cajanus. In cultivated pigeonpea, chickpea-specific SSRs showed 38–39% transferability, while among wild Cajanus species, it ranged from 26% in Cajanus sericeus ICP 15760 to 40% in C. sericeus ICP 15761. The transferable primers exhibited extensive polymorphism in Cajanus with an average number of 4.11 alleles per marker. High level of polymorphism exhibited by chickpea microsatellite markers in the present study indicates their usefulness in diversity analysis, mapping agronomically important traits and marker-assisted breeding in pigeonpea.Not Availabl
Development of an efficient Agrobacterium mediated transformation system for chickpea (Cicer arietinum)
No full textCritical considerations and computational tools in plant genome editing
No full textRecent advances in genome editing tools and CRISPR-Cas technologies have enabled plant genome engineering reach new heights. The current regulatory exemptions for certain categories of genome edited products, such as those derived from SDN-1 and SDN-2, which are free of any transgene, have significantly accelerated genome editing research in a number of agricultural crop plants in different countries. Although CRISPR-Cas technology is becoming increasingly popular, it is still important to carefully consider a number of factors before planning and carrying conducting CRISPR-Cas studies. To attempt genome editing in a plant, a high-quality genome sequence and a repeatable tissue culture protocol for in vitro regeneration are essential. One of the most important steps in plant genome editing is the designing of a CRISPR construct, which involves selecting the appropriate Cas protein, sgRNA sequence, and appropriate regulatory sequence to trigger expression. Computational tools and algorithms play a crucial role in construct design and gRNA selection to minimize off-target effects and also to optimize their delivery techniques. Researchers may need to select appropriate software tools capable of analyzing post-editing detection of mutation events and other DNA sequence abnormalities to identify off-target effects. To fully fulfill the potential of plant genome editing, continued advances in computational biology are essential to meet the challenges it faces today
Isolation and Characterization of Starch from Moth Bean (Vigna Aconitifolia)
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
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