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Molecular insights of fungal endophyte co-inoculation with Trichoderma viride for the augmentation of forskolin biosynthesis in Coleus forskohlii
No full textMastan, Anthati, Rane, Digeshwar, Dastager, Syed G., Babu, C.S. Vivek (2021): Molecular insights of fungal endophyte co-inoculation with Trichoderma viride for the augmentation of forskolin biosynthesis in Coleus forskohlii. Phytochemistry (112654) 184: 1-10, DOI: 10.1016/j.phytochem.2021.112654, URL: http://dx.doi.org/10.1016/j.phytochem.2021.11265
Trichoderma viride
No full text5.3. Co-inoculation of endophytic fungi with Trichoderma viride Prior to co-inoculation studies, compatibility between each fungal endophyte and T. viride was tested using dual culture method and found no antagonism between endophytes and T. viride. Further, co-inoculation studies were carried out under field conditions at CSIR–CIMAP Research Centre, Bangalore in an experimental plot (3 × 3 m) of red-sandy loom soil (pH 6.2). Thirty-days-old nursery plants of C. forskohlii were transplanted into 10–12 cm deep holes with spacing of 60 × 60 cm between plants. Four rows were maintained in each treatment plot (total of 8 plots) and divided by a guard ridge (50 cm width) to avoid direct contact with adjacent plots. Each experimental plot was planted with 16 plants in four rows (hence, 4 plants were in the middle surrounded by 12 peripheral plants). The four central plants (four replicates) from each treatment were selected for the measurement of growth parameters. The fungal cultures were grown in 500 mL potato dextrose broth for 7 days at 28 ◦ C and the resulting mycelia were diluted with 90 mL of phosphate buffered saline (PBS) (K2HPO4 0.24 g L 1, Na2HPO4 1.44 g L 1, KCl 0.2 g L 1, NaCl 8 g L 1 and pH 7.40). Finally, fungal suspension was diluted to a density of 1 × 108 CFU mL 1. After 30 days of transplantation, each plant in the experimental plot was treated with 30 mL inoculum of SF1, SF2, RF 1 or TV1 and the untreated experimental plot (n = 16 plants) was considered as control. Co-inoculation treatments included RF 1+TV1, SF1+TV1, and SF2+TV1 at an inoculum of 1:1 ratio (Egamberdieva et al., 2017). For each treatment plot, plant growth measurements comprising number of branches and plant height were analyzed for every 30 days until harvest. After 150-days of transplantation, plants were harvested by manually uprooting each plant with utmost care so as to not damage the roots. Fresh weights of roots and shoots were measured at the time of harvest and dry weights of roots and shoots were measured after 4-weeks of drying period; root length and number of tuberous roots per plant were also recorded. The obtained values of endophyte treatments (alone and co-inoculum) were compared with endophyte uninoculated control plants.Published as part of Mastan, Anthati, Rane, Digeshwar, Dastager, Syed G. & Babu, C. S. Vivek, 2021, Molecular insights of fungal endophyte co-inoculation with Trichoderma viride for the augmentation of forskolin biosynthesis in Coleus forskohlii, pp. 1-10 in Phytochemistry (112654) (112654) 184 on page 8, DOI: 10.1016/j.phytochem.2021.112654, http://zenodo.org/record/829227
Trichoderma viride
No full text2.2. Synergistic effect of fungal endophytes and T. viride on plant growth of C. forskohlii Co-inoculation of RF 1, SF1, or SF2 fungal endophyte with T. viride modulates C. forskohlii plant growth. Significant enhancement in branch number and plant height was noticed in both individual (endophyte or T. viride) and co-inoculated treatments (endophyte +TV1) compared to the uninoculated control plants. However, more enhancements in plant height and branch numbers were noticed in co-inoculations compared to individual treatments (Fig. S2). C. forskohlii treated with RF 1, SF1, SF2, and TV1 showed 29–54% and 21–33% more branch number and height, respectively, compared to control plants. The plants co-inoculated with RF 1+TV1, SF1+TV1, and SF2+TV1 resulted in significant improvement of plant height (54, 41, and 52%, respectively) and number of branches (75, 67, and 70%, respectively) compared to control plants (Fig. 2a and b). Fresh and dry mass of root and shoot were significantly higher in co-inoculated plants compared to individual treatments and control plants. However, individual treatments showed more fresh and dry mass of root and shoot compared to control plants. The plants inoculated with RF 1+TV1, SF1+TV1, and SF2+TV1 resulted in significant increase of fresh shoot weight at 100, 70, and 97%, fresh root weight at 73, 44, and 55%, dry shoot weight at 67, 32, and 56%, and dry root weight at 94, 53, and 69%, respectively compared to control plants. Similarly, treatment of C. forskohlii with RF 1, SF2, and TV1 resulted in significant enhancement of root length (20, 27, and 37%, respectively) and number of tuberous roots (37, 27, 24, and 20%, respectively) compared to control plants (Fig. 2c–f). However, SF1 did not significantly enhance root length (7%). The plants treated with a consortium of RF 1+TV1, SF1+TV1, or SF2+TV1 resulted in significant increment of root length (41, 16, or 37%, respectively) and number of tuberous roots (74, 35, or 55%, respectively) compared to control plants (Fig. 2g and h). Overall, the effect of endophytes and TV 1 in different combinations (individual and co-inoculation) revealed that the inoculation of RF 1+TV1 combination showed significant improvement in all growth measurements, followed by combination of SF2+TV1 compared to other treatments and control plants.Published as part of Mastan, Anthati, Rane, Digeshwar, Dastager, Syed G. & Babu, C. S. Vivek, 2021, Molecular insights of fungal endophyte co-inoculation with Trichoderma viride for the augmentation of forskolin biosynthesis in Coleus forskohlii, pp. 1-10 in Phytochemistry (112654) (112654) 184 on page 2, DOI: 10.1016/j.phytochem.2021.112654, http://zenodo.org/record/829227
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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