172 research outputs found
Supplemental Material - Effects of Gehl’s urban design guidelines on walkability: A virtual reality experiment in Singaporean public housing estates
No full textSupplemental Material for Effects of Gehl’s urban design guidelines on walkability: A virtual reality experiment in Singaporean public housing estates by Heidi Silvennoinen, Saskia Kuliga, Pieter Herthogs, Daniela Rodrigues Recchia and Bige Tunçer in Environment and Planning B: Urban Analytics and City Science</p
An efficient in vitro transposition method by a transcriptionally regulated sleeping beauty system packaged into an integration defective lentiviral vector
No full textThe Sleeping Beauty (SB) transposon is a non-viral integrating system with proven efficacy for gene transfer and functional genomics. To optimize the SB transposon machinery, a transcriptionally regulated hyperactive transposase (SB100X) and T2-based transposon are employed. Typically, the transposase and transposon are provided transiently by plasmid transfection and SB100X expression is driven by a constitutive promoter. Here, we describe an efficient method to deliver the SB components to human cells that are resistant to several physical and chemical transfection methods, to control SB100X expression and stably integrate a gene of interest (GOI) through a "cut and paste" SB mechanism. The expression of hyperactive transposase is tightly controlled by the Tet-ON system, widely used to control gene expression since 1992. The gene of interest is flanked by inverted repeats (IR) of the T2 transposon. Both SB components are packaged in integration defective lentiviral vectors transiently produced in HEK293T cells. Human cells, either cell lines or primary cells from human tissue, are in vitro transiently transduced with viral vectors. Upon addition of doxycycline (dox, tetracycline analog) into the culture medium, a fine-tuning of transposase expression is measured and results in a long-lasting integration of the gene of interest in the genome of the treated cells. This method is efficient and applicable to the cell line (e.g., HeLa cells) and primary cells (e.g., human primary keratinocytes), and thus represents a valuable tool for genetic engineering and therapeutic gene transfer
Una riconsiderazione sull’uso delle strutture protoappenniniche di Torre dei Passeri (PE)
No full textA few rock-cut structures, dated back to the first half of the second millennium BC, were recently excavated and published. They were especially found in Daunia and they are generally considered places of worship. Each of them has specific features, but it seems that they form part of a common “hypogeic worship”. Starting from these evidence, author deals with a new analysis of well known contexts (e.g. Torre dei Passeri, structure B), suggesting that they should take part in the same phenomenon
L'effettività del diritto antidiscriminatorio dentro e oltre il processo. Introduzione
No full textIl contributo introduce un numero monografico della Rivista, dedicato all’approfondimento sullo stato dell’arte e sulle tendenze delle tecniche di tutela nel contrasto alle discriminazioni, partendo dalla tradizionale distinzione tra rimedi giudiziali e vie alternative di prevenzione, attivazione e soluzione dei conflitti. Se il diritto antidiscriminatorio è divenuto negli ultimi anni oggetto di attenzioni particolari, se non inedite, certamente singolari per la loro scala, si tratta comunque di un diritto complesso e dinamico, di cui è necessario saggiare concretamente e a più riprese il grado di efficacia, affidato com’è non solo al suo solido impianto teorico, ma soprattutto alla sua interpretazione e alla sua pratica.The contribution introduces a monographic issue of the Journal, dedicated to an in-depth examination of the state of the art and trends in protection techniques in the fight against discrimination, starting from the traditional distinction between judicial remedies and alternative ways of preventing, activating and resolving conflicts. If anti-discrimination law has become the object of special attention in recent years, if not unprecedented, certainly singular in its scale, it is nonetheless a complex and dynamic law, whose degree of effectiveness must be concretely and repeatedly tested, entrusted as it is not only to its solid theoretical framework, but above all to its interpretation and practice
Antenati, “eroi”, nemici. Sepolture e resti umani in alcuni abitati dell’età del Bronzo nell’Italia peninsulare
No full textThe finding of human remains inside the Bronze Age settlements of the Italian peninsula is not so frequent,
but their presence in some of them is an element of great interest. It can be variously interpreted.
The ways of treatment of the human remains are different, also inside the same settlement: there are real
burials, also with grave goods (for example inside the posterns of the walls at Coppa Nevigata - Foggia),
whole skeletons to link to fortuitous death because of their context (for examples the individuals
unearthed inside a postern of the walls of Roca Vecchia - Lecce) and sporadic bones in specific areas of
the settlement. The differences are to relate to the conditions of death, to the funerary rituals and, first
of all, to the categories of individuals (as ancestors, «heroes», enemies) that were variously treated on
the occasion of their burial. Author aims at outlining the different patterns of behaviour (to link to specific
meanings) in the archaeological contexts taken into consideration
Le pari opportunità dopo il Jobs Act 2: organismi e azioni positive
No full textIl saggio affronta il tema dell'evoluzione della normativa in tema di pari opportunità, dalle origini al Jobs Ac
Che cos'è la verità? Il silenzio di Gesù, l'eloquenza del diritto, e le soluzioni delle democrazie contemporanee in tema di negazionismo
No full textSe la verità gode del lusso dell'immediatezza nei Vangeli, essa si fa invece problematica nel diritto, dove pretende di essere pensata ed espressa con maggiore profusione di argomenti. Se guardato dal punto di vista del diritto, quell'interrogativo non è né sciocco (secondo Kierkegaard, la domanda di Pilato “non poteva essere più sciocca: non perché egli chieda cos'è la verità, ma perché lo domanda a Cristo”), né giocoso (Francis Bacon liquiderà altrettanto decisamente la questione, parafrasando quel passo evangelico attraverso l'espressione “disse scherzando” -“said jesting Pilate”-).
Ai nostri fini, torna più utile isolare la riflessione di Kelsen, che commenta il dialogo tra Pilato e Gesù in questi termini : “Poiché egli (Pilato), relativista scettico, non sapeva cosa fosse la verità, la verità assoluta in cui quell'uomo credeva, agì in modo democratico -con assoluta coerenza- rimettendo la decisione del caso al voto del popolo
Specific knock-down of C-terminal dominant mutation in Rhodopsin gene by CRISPR/Cas9 system
No full text
La discriminazione per orientamento sessuale e identità di genere come “fondata sul sesso”: la Corte Suprema degli Stati Uniti rilegge il diritto del lavoro federale
No full textIn Bostock v. Clayton County, the Supreme Court held that Title VII of the Civil Rights Act of 1964, which prohibits sex discrimination in employment, covers discrimination on the basis of sexual orientation and gender identity. The Author tracks the judicial route that led to the decision, the textual approach adopted by the Court in the interpretation of the expression “because of sex” and offers some offers some remarks on the prospects for anti-discriminatory law, also at European level.Nella sentenza Bostock v. Clayton County, la Corte Suprema degli Stati Uniti ha stabilito che il Titolo VII del Civil Rights Act del 1964, che nei rapporti di lavoro vieta la discriminazione a causa del sesso, è invocabile anche nei casi di discriminazione per orientamento sessuale e identità di genere. L’A. ricostruisce il percorso giurisprudenziale che ha aperto le porte alla decisione, l’approccio testuale adottato dalla Corte nell’interpretazione dell’espressione “in ragione del sesso” e offre alcune spunti sulle prospettive, anche europee, del diritto antidiscriminatorio
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