62 research outputs found
Nuove prospettive su canone e commento dei canzonieri quattrocenteschi
No full textL’intervento fa seguito della pubblicazione dell’ “Atlante dei canzonieri in volgare del Quattrocento”, curato da Andrea Comboni e dal sottoscritto, uscito nel 2017 per i tipi della Sismel-Edizioni del Galluzzo di Firenze. L’ipotesi di partenza è che la pubblicazione dell’ “Atlante” abbia portato a delle novità in fatto di canone e di commento nella poesia quattrocentesca, nella fattispecie nella lirica quattrocentesca orga¬nizzata in canzonieri. Dalla posizione restrittiva e limitativa di Benedetto Croce, attraverso le antologie quattrocentesche di Contini e di Segre - Ossola, l’arrivo dell’ “Atlante” ha comportato un allargamento, ridefinizione e precisazione del canone della poesia di un secolo, a torto ritenuto privo di veri lirici
Strategie di ricomposizione macrotestuale nei canzonieri (o canzoniere?) di Gaspare Ambrogio Visconti
No full textThis paper examines the private poetic production of Gaspare Ambrogio Visconti, i.e. the two canzonieri dedicated to Beatrice d’Este and Bianca Maria Sforza, and explores the strategies that the author has implemented in transforming the former into the latter, which is basically an enlargement. It concludes by establishing the primacy of the canzoniere to Beatrice between the two
Rhabdomyosarcoma of the skin resembling carcinosarcoma: Report of a case and literature review
No full textRhabdomyosarcomas (RMSs) are soft tissue sarcomas with skeletal muscle differentiation. Cutaneous RMSs are exceedingly rare, and in most cases, they represent metastatic disease or the dermal involvement by a tumor arising in the underlying soft tissues. We herein report the case of a 41-year-old man who developed a cutaneous swelling of the right nasal orbital angle. An initial incisional biopsy showed cytokeratin-positive atypical spindle cells forming long and intersecting fascicles, thus the case was diagnosed as carcinosarcoma. In the subsequent excisional biopsy, the skeletal myogenic differentiation of the tumor cells infiltrating the dermis and subcutis was demonstrated by morphology (presence of rhabdomyoblasts), immunohistochemistry (positivity for desmin, myogenin, myoglobin, and actins), and electron microscopy (evidence of rudimentary sarcomeric structures). A final diagnosis of primary cutaneous RMS was made. The patient was subjected to postoperative radiation and chemotherapy, but after 4 months, the patient developed a tumor recurrence followed by distant metastases and death. Review of the literature reveals that RMSs of the skin are often underrecognized and display peculiar clinical features in comparison with their more common soft tissue counterpart. Copyright © 2012 by Lippincott Williams & Wilkins
Morphologic shift associated with aberrant cytokeratin expression in a GIST patient after tyrosine kinase inhibitors therapy. A case report with a brief review of the literature
No full textAfter an initial benefit from tyrosine kinase inhibitors (TKI), most gastrointestinal stromal tumors (GISTs) eventually develop disease progression or secondary resistance. An altered tumor (immune)phenotype with anaplasia and morphological changes secondary to therapy have occasionally been described in the literature.We present a 52-year old patient, diagnosed with high risk, spindle-cell, GIST (CD117 positive, Pankeratin negative) in 2003, showing a c-Kit exon 11 mutation. After TKI therapy, he developed drug resistance and disease progression. Pathological assessment of the last surgical specimen showed a pure epithelioid/clear cell histology, without evidence of cellular anaplasia. Tumor cells were CD117 positive, DOG1 positive but also E-cadherin positive and Pankeratin positive, whereas molecular analysis confirmed the presence of the c-Kit exon 11 mutation, with no additional mutations. We describe an unusual case of GIST showing peculiar (immuno)phenotypic changes under therapy, different from the vast majority of therapy-driven changes, which include marked cellular pleomorphisms and KIT immunonegativity. Possible molecular explanations to understand these phenomena and a brief review of the literature are also addressed. (C) 2015 Elsevier GmbH. All rights reserved
Primary effusion lymphoma cell lines harbouring human herpesvirus type-8
No full textPrimary effusion lymphoma (PEL) is a novel lymphoma entity consistently infected by HHV-8 that occurs predominantly in immunodeficient patients and is characterized by liquid growth in the serous body cavities. in order to facilitate the understanding of PEL pathogenesis and histogenesis, we have established three PEL cell lines termed CRO-AP/2, CRO-AP/3 and CRO-AP/5. All cell lines have been derived from HIV positive homosexual men affected by PEL with tin the case of CRO-AP/2 and CRO-AP/5) or without tin the case of CRO-AP/3) a previous history of Kaposi's sarcoma. The cell lines are representative of both virologic variants of PEL, i.e. HHV-8(+) EBV+ PEL (CRO-AP/2 and CRO-AP/5) and HHV-8(+) EBV- PEL (CRO-AP/3). Morphologic and phenotypic features of CRO-APR, CRO-AP/3 and CRO-AP/5 are typical of PEL, and include morphology bridging immunoblastic and anaplastic features as well as an indeterminate (non B- non T-cell) phenotype. The B-cell nature of the cell lines is documented by the presence of rearranged immunoglobulin genes. The detailed analysis of the molecular and phenotypic features of CRO-AP/2, CRO-AP/3 and CRO-AP/5 has allowed the identification of recurrent chromosomal abnormalities of PEL and has contributed to the definition of PEL as a lymphoma of post-germinal center, pre-terminally differentiated B-cells
DIFFERENTIAL EXPRESSION OF BCL-6, CD138/SYNDECAN-1 AND EBV-ENCODED LATENT MEMBRANE PROTEIN-1 IDENTIFIES DISTINCT HISTOGENETIC PATHWAYS OF AIDS-RELATED NON-HODGKIN's LYMPHOMAS
No full textThis study was aimed at defining the histogenesis of the pathologic spectrum of acquired immunodeficiency syndrome-related non-Hodgkin's lymphomas (AIDS-NHL), including AIDS-related small noncleaved cell lymphoma (AIDS-SNCCL), AIDS-related large noncleaved cell lymphoma (AIDS-LNCCL), AIDS-related large cell immunoblastic lymphoma plasmacytoid (AIDS-IBLP), and AIDS-related primary effusion lymphoma (AIDS-PEL). Forty-six cases of AIDS-NHL were investigated for the expression pattern of BCL-6, a protein specifically expressed by germinal center (GC) B-cells, and CD138/syndecan-1 (syn-1), a marker of post-GC B-cell differentiation. Expression of BCL-6 and syn-1 segregated two major phenotypic patterns among AIDS-NHL: (1) the BCL-6+/syn-1- pattern associated with AIDS-SNCCL and AIDS-LNCCL; (2) the BCL-6-/syn-1+ pattern associated with AIDS-IBLP and AIDS-PEL. Among systemic AIDS-NHL infected by Epstein-Barr virus (EBV), expression of the EBV-encoded latent membrane protein-1 (LMP-1) preferentially associated with the BCL-6-/syn-1+ profile. Analysis of nonneoplastic lymph nodes showed that the two phenotypic patterns detected in AIDS-NHL correspond to physiologic stages of B-cell development, i.e., GC B-cells (BCL-6+/syn-1-) and preterminally differentiated post-GC B-cells (BCL-6-/syn-1+). Thus, BCL-6+/syn-1- AIDS-NHL reflects a GC stage of differentiation, whereas AIDS-NHL which are BCL-6-/syn-1+, and LMP-1+ when infected by EBV, derive from B cells that have entered post-GC plasmacell differentiation. These findings are relevant for the pathogenesis and differential diagnosis of AIDS-NHL
Expression profile of MUM1/IRF4, BCL-6, and CD138/syndecan-1 defines novel histogenetic subsets of human immunodeficiency virus-related lymphomas
No full textThis study was aimed at defining the histogenesis of the pathologic spectrum of lymphoma arising in the context of human immunodeficiency virus (HIV) infection. Toward this aim, 87 AIDS-related non Hodgkin lymphomas (AIDS-NHL) and 16 Hodgkin lymphomas arising in HIV+ patients (HIV-HL) were comparatively analyzed for the expression pattern of several B-cell histogenetic markers, including BCL-6 (expressed by centroblasts and centrocytes), MUM1/IRF4 (expressed by late centrocytes and post-germinal center [GC] B cells), and CD138/syn-1 (expressed by post-cc B cells). Expression of MUM1, BCL-6, and syn-1 segregated 3 major phenotypic patterns among AIDS-NHL and HIV-HL: (1) the BCL-6(+)/MUM1(-)/ syn-1(-) pattern, selectively clustering with a large fraction of AIDS-Burkitt lymphoma (17 of 19) and of systemic AIDS-diffuse large cell lymphoma (12 of 16); (2) the BCL-6(-)/MUM1(+)/syn-1(-) pattern, associated with a fraction of AIDS-immunoblastic lymphoma (8 of 24); and (3) the BCL-6(-)/ MUM1(+)/syn-1(+) pattern, associated with systemic and primary central nervous system immunoblastic lymphoma (14 of 24) and with primary effusion lymphoma (10 of 10), plasmablastic lymphoma of the oral cavity (7 of 7), and HIV-HL (15 of 16), Analysis of nonneoplastic lymph nodes showed that the 3 phenotypic patterns detected in AIDS-NHL and HIV-HL correspond to distinct stages of physiologic B-cell developemtn-centroblasts (BCL-6(+)/MUM1(-)/syn1(-)), late GC/early post-cc B cells (BCL-6(-)/MUM1(+)/syn-1(-)), and post-GC B cells (BCL-6(-)/MUM1(+)/syn-1(+)). Expression of the Epstein-Barr virus-encoded latent membrane protein-1 clustered with the BCL-6(-)/MUM1(+)/syn-1(+) profile throughout the clinicopathologic spectrum of AIDS-NHL and HIV-HL, Overall, these results define novel histogenetic subsets of AIDS NHL and HIV-HL and may provide novel tools for refining the diagnosis of these disorders. (C) 2001 by The American Society of Hematology. RI Larocca, Luigi/A-1577-200
Genetic heterogeneity of AIDS-related small non-cleaved cell lymphoma
No full textAIDS-related small noncleaved cell lymphoma (AIDS-SNCCL) includes Burkitt's lymphoma (BL) and high-grade B-cell Burkitt-like lymphoma (BLL). Due to the marked polymorphism of AIDS-related non-Hodgkin's lymphomas (AIDS-NHL), the morphologic distinction between these two types of lymphomas is frequently controversial, although it may bear clinical relevance. Although the molecular features of AIDS-BL have been clarified to a certain extent, the genetic peculiarities of AIDS-BLL have not been investigated in detail. In this study we have compared morphologic and genetic features of AIDS-BL and AIDS-BLL in a blind coded fashion. Molecular studies were focused on the genetic lesions known to be implicated in AIDS-NHI, including alterations of c-MYC, BCL-6, p53, deletions of 69, as well as infection by EBV and HHV-8. Alterations of c-MYC occurred in 10/10 AIDS-BL, whereas they were restricted to 2/10 AIDS-BLL (P<0.01). Mutations of p53 were present in 5/10 AIDS-BL, whereas they were consistently absent among AIDS-BLL (n = 10; P < 0.05). Infection by EBV occurred in 30% of both AIDS-BL and AIDS-BLL. Rearrangements of BCL-6, deletions of 6q and infection by HHV-8 scored consistently negative in both AIDS-BL and AIDS-BLL. Based on the genetic lesions tested, the molecular profile of AIDS-BLL appears to be closer to that of AIDS-related diffuse large cell lymphoma (AIDS-DLCL) than to that of AIDS-BL. In contrast to AIDS-BLL, however, AIDS-DLCL carried rearrangements of BCL-6 in a fraction of cases (2/9). This study, the largest of its kind reported so far, suggests that AIDS-BL and AIDS-BLL have a different molecular pathogenesis and that characterization of genetic lesions may help to distinguish between these two lymphomas
Label-free electrochemical aptasensor for the detection of SARS-CoV-2 spike protein based on carbon cloth sputtered gold nanoparticles
No full textThe proliferation and transmission of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), or the (COVID-19) disease, has become a threat to worldwide biosecurity. Therefore, early diagnosis of COVID-19 is crucial to combat the ongoing infection spread. In this study we propose a flexible aptamer-based electrochemical sensor for the rapid, label-free detection of SARS-CoV-2 spike protein (SP). A platform made of a porous and flexible carbon cloth, coated with gold nanoparticles, to increase the conductivity and electrochemical performance of the material, was assembled with a thiol functionalized DNA aptamer via S–Au bonds, for the selective recognition of the SARS-CoV-2 SP. The various steps for the sensor preparation were followed by using scanning electron microscopy, cyclic voltammetry and differential pulse voltammetry (DPV). The proposed platform displayed good mechanical stability, revealing negligible changes on voltammetric responses to bending at various angles. Quantification of SARS-CoV-2 SP was performed by DPV and chronopotentiometry (CP), exploiting the changes of the electrical signals due the [Fe(CN)6]3-/4- redox probe, when SARS-CoV-2 SP binds to the aptamer immobilized on the electrode surface. Current density, in DPV, and square root of the transition time, in CP, varied linearly with the log[ SARS-CoV-2 SP], providing lower limits of detection (LOD) of 0.11 ng/mL and 37.8 ng/mL, respectively. The sensor displayed good selectivity, repeatability, and was tested in diluted human saliva, spiked with different SARS-CoV-2 SP concentrations, providing LODs of 0.167 ng/mL and 46.2 ng/mL for DPV and CP, respectively
Antologizzare il proprio passato. Macrostrutture liriche e temporalità del lutto
No full textPsychoanalytic Nachträglichkeit appears to be the temporality that structures the canzonieri in morte, that is to say, collections of poems devoted to the poet’s dead beloved. In fact, a similar process of (self)-interpretation that is both retrospective and progressive at the same time can be found much earlier in European poetry. The article starts from certain theoretical reflections and an overview of the two fundamental models provided by Dante’s Vita Nova and Petrarch’s Canzoniere in order to focus on some poems from two contemporary Italian canzonieri in morte: Eugenio Montale’s Xenia (in Satura, 1971) e Milo De Angelis’ Tema dell’addio (2005). The intent is to show how the complex temporality that Freud named Nachträglichkeit can perform different essential functions: to organize the lyric macro-text; to construct a new meaning for poems previously composed; to reconsider the poet’s own personal and poetic past; and to contribute to the creation of a new author. Certain features delineated in the canzonieri will finally lead to reflect upon the ‘self-anthology’ as a form, that is to say, an anthology of the poet’s work selected and organized by the poet him/herself
- …
