201,288 research outputs found
Minh Anh Dang Trinh
No full text학위논문(석사)--아주대학교 일반대학원 :의학과,2009. 2TABLE OF CONTENTS
ABSTRACT................i
TABLE OF CONTENTS.................ii
LIST OF FIGURES.......v
LIST OF TABLE......... vi
I. INTRODUCTION...... 1
A. Morphology........... 1
B. Life cycle.............. 4
C. Diagnosis............. 7
D. Lactate dehydrogenase of parasites......... 7
E. Purpose of this study............. 8
II. MATERIALS AND METHODS... 9
A. Culture of myeloma cells........ 9
B. Antigen..9
C. Immunization of mice with TgLDH........... 9
D. Cell fusion.............10
E. ELISA screening.... 11
F. Selection of hybridoma cells....11
G. Large scale production of McAbs............ 12
H. Monoclonal antibody isotyping.12
III. RESULTS..............13
A. Antibody formation of immunized mice 13
B. Cell fusion.............15
C. Isolation of antibody-producing hybridoma cells........ 16
D. Producing large scale of antibodies..........17
E. The specificity of McAbs with T. gondii lysate...........18
F. Isotyping of monoclonal antibodies............20
IV. DISCUSSION........ 21
V. CONCLUSION........23
REFERENCES........... 24
국문요약....29
LIST OF FIGURES
Fig. 1. Morphology of T. gondii.... 3
Fig. 2. Life cycle of T. gondii ...... 6
Fig. 3. Polyclonal antibody titers from the TgLDH-5-immunized mice........ 14
Fig. 4. Antibody titers of the the hybridoma cell culture supernatant after cell fusion ........15
Fig. 5. Antibody titers of the hybridoma cell culture supernatant after third limiting dilution .......... 16
Fig. 6. Antibody titers of McAb-containing ascitic fluids.. 17
Fig. 7. Antibody titers of the hybridoma cell culture supernatant using T. gondii lysate as antigen................18
Fig. 8. Antibody titers of McAb-containing ascitic fluids using T. gondii lysate as antigen..19
LIST OF TABLE
Table 1. Amino acids sequence of synthetic peptide for TgLDH-5... 9
Table 2. Isotype profile of eight hybridoma clones producing monoclonal antibody...20MasterBackground/Purpose: Toxoplasma gondii is one of the most common parasitic infections of human and other warm blooded animals. Because of the geographical distribution of toxoplasmosis, there is a need to develop a sensitive, rapid, simple and an inexpensive test for the early screening of patiens. In this study, to create the dipstick kit that has high sensitivity against LDH (lactate dehydrogenase) of T. gondii, at the first of all, hybridoma cell lines producing monoclonal antibodies with high affinity and specificity against T. gondii LDH (TgLDH) were established.
Methods: After 6-8 week old BALB/c mice were immunized with synthetic peptides of TgLDH, splenocytes were harvested. To get the monoclonal antibodies (McAbs), splenocytes were fused with myeloma cells using PEG by a cell fusion technique. Hybridoma cells were selected by ELISA and subcloned through a limiting dilution method. McAbs were characterized by isotyping.
Results: Eight McAb-producing hybridoma cells were selected. After three times of limiting dilution, eight clones, 1A10, 1B12, 3B2, 3B6, 1C12, 3C8, 3G6 and 3H1, were selected. OD values of eight monoclonal antibodies were ranged from 0.938 to 1.269 by ELISA. All eight McAbs isotypes were IgM class.
Conclusion: These results indicated that eight hybridoma clones had strong reactivity to TgLDH and may be useful for diagnosis to T. gondii infection
The Hard Science Spoken Word List (Dang, 2018)
No full textA Hard Science Spoken Word List (HSWL) was developed and validated to help second language learners of hard sciences better comprehend academic speech at English-medium universities. It consists of the 1,595 most frequent and wide ranging word families in a 6.5-million running word hard science spoken corpus which represents 12 subjects across two equally-sized sub-corpora. Its coverage in different discourse types indicates that the HSWL truly reflects the language in hard science academic speech. The comparison between the HSWL with Dang, Coxhead, and Webb’s (2017) Academic Spoken Word List shows that the HSWL focuses more on specialized vocabulary in hard science speech. Depending on their vocabulary levels, learners may achieve 93%–96% coverage of hard science academic speech with knowledge of the HSWL words. Souce: Dang, T. N. Y. (2018). The hard science spoken word list. ITL – International Journal of Applied Linguistics, 169(1), 44–71. https://doi.org/10.1075/itl.00006.da
Charlie May Simon materials
No full textThis collection contains materials relating to Arkansas author Charlie May Simon
Lasianthus yaharae V. S. Dang, Tagane & H. Tran 2015
No full text<p> <b>74.</b> <i>Lasianthus yaharae</i> V.S.Dang, Tagane & H.Tran (2015: 352) — Fig. 107 (plate), Fig. 104 (map)</p> <p> <b>Type:</b> — VIETNAM. Khanh Hoa Province, Hon Ba Nature Reserve, 1521 m elev., 18 July 2014, <i>Tagane S., Kanemitsu H., Son V.D., Tran H. with Loi X.N., Thach N.D., Dinh N. & Hieu P.N.H. V1741</i> (holotype VNM! [VNM00042947]; isotypes FU!, KYO!, VNM! [VNM00042948, VNM00042949], Station of Hon Ba Nature Reserve).</p> <p> <b>Specimens examined:</b> — VIETNAM. <b>Central:</b> Khanh Hoa Province, Hon Ba Nature Reserve, 30 km west of Nha Trang City, 1521 m elev., 2 November 2012, <i>Son V.D., Tagane S., Toyama H., Fuse K., Nagamasu H., Hop T., Lam T.T. & Yahara T. VHB44</i> (VNM); in the same location, 1524 m elev., 28 November 2017, <i>Dang V.S. & Naiki A. N10114</i> (VNM, the herbarium of Iriomote Station (Japan)); in the same location, 1520 m elev., 26 July 2018, <i>Dang V.S. & Hoang N.T.B.N., Dang 198</i> (VNM, the herbarium of Iriomote Station (Japan)).</p> <p> <b>Distribution:</b> —Endemic to Vietnam (currently known as Hon Ba Nature Reserve of Khanh Hoa Province and Bidoup Nui Ba National Park of Lam Dong Province).</p> <p> <b>Habitat & Ecology:</b> —Growing in moist evergreen forest, dominated by members of the Fagaceae family at 1400–1600 m elev.</p> <p> <b>Phenology:</b> —Flowering and fruiting from May to January of the next year.</p> <p> <b>Vernacular name:</b> —Xú hưƠng yahara.</p> <p> <b>Notes:</b> — <i>Lasianthus yaharae</i> was described as new from a Vietnamese specimen (<i>V1741</i>) by Dang <i>et al.</i> (2015). This species morphologically resembles <i>L. dalatensis</i> Wernham (1921: 138) which is endemic to Vietnam, but differs from the latter in having secondary veins 4–5 on each side of midrib (vs. 7–9 on each side of midrib), flowers pinkish at anthesis (vs. white), and calyx 2–3 mm long (vs. 1 mm long), corolla tube 5–10 mm long (vs. 4 mm long) and corolla lobes 2–4 mm long (vs. 1.5 mm long).</p>Published as part of <i>Dang, Van-Son & Naiki, Akiyo, 2023, A revision of the genus Lasianthus Jack (Rubiaceae) from Vietnam, pp. 1921-1935 in Phytotaxa 581 (1)</i> on pages 1921-1935, DOI: 10.11646/phytotaxa.581.1.1, <a href="http://zenodo.org/record/7571550">http://zenodo.org/record/7571550</a>
Secondary Literacy Across the Curriculum
No full textThis paper discusses the challenges and possibilities attendant upon successfully implementing literacy across the curriculum initiatives – or ‘school language policies’ as they have come to be known – particularly at the secondary or high school level. It provides a theoretical background to these issues, exploring previous academic discussions of school language policies, and highlights key areas of concern as well as opportunity with respect to school implementation of such policies. As such, it provides a necessary conceptual background to the subsequent papers in this special issue, which focus upon the Secondary Schools’ Literacy Initiative (SSLI) – a New Zealand funded programme that aims to establish cross-curricular language and literacy policies in secondary schools
The construction of Karen Karnak: The multi-author-function
No full textThis thesis is situated within the comparatively recent developments of Web 2.0 and the emergence of interactive WikiMedia, and explores the mode of authorship within a Read/Write culture compared to that of a Read/Only tradition. The hypothesis of this study is that the role of the audience has become merged with the author, and as such, represents new functions and attributes, distinct from a more conventional concept of authorship, in which the roles of audience and author are more separate. Read/Write and participatory culture, as defined by this study, is focused on collaboration, and includes the influences of D.I.Y. culture, Open-Source practices and the production of text by multiple authors. Multi-authorship presents a re-thinking of several concepts which support the notion of the individual author, since the focus of multi-authorship is not on attribution and ownership of a finished text, but on the continued malleability of a text. Modes of multi-authorship, demonstrated in the use of the pseudonyms Alan Smithee and Karen Eliot, represent declarative authors whose names signify multiple origins, whilst concurrently indicating a distinct body of work. The function of these names form an important context to this study, since primary research involves the construction of an experimental mode of multi-authorship utilising WikiMedia technology and the interaction of thirty nine participants, who are invited to create a body of work under the collective pseudonym Karen Karnak. The data generated by this experiment is analysed using aspects of Michel Foucault's author-function to identify and determine power structures inherent in the WikiMedia context. The interplay of power structures, including concepts such as identity, ownership and the body of work, affect the resulting mode of authorship and contribute to the construction of Karen Karnak, suggesting further areas of research into the emerging multi-author
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
The relationship between the Dang Van criterion and the traditional bulk fatigue criteria
No full textA formal relationship is established between the traditional bulk fatigue criteria such as the Goodman rule and the Sines criterion, and the recent crack initiation criterion of Dang Van. The constants implied by the Dang Van procedure may be formally connected to the fatigue limit and mean stress dependence given by the Goodman rule, under conditions of uniaxial loading. The biaxial fatigue criterion of Sines is also compared with the Dang Van procedure. The similarity in the approaches may be further extended to permit finite initiation times to be estimated by the Dang Van criterion when the fatigue limit is exceeded
Modeling And Control Battery Aging in Energy Harvesting Systems
No full textEnergy storage is a fundamental component for the development of sustainable and environment-aware technologies. One of the critical challenges that needs to be overcome is preserving the State of Health (SoH) in energy harvesting systems, where bursty arrival of energy and load may severely degrade the battery. Tools from Markov process and Dynamic Programming theory are becoming an increasingly popular choice to control dynamics of these systems due to their ability to seamlessly incorporate heterogeneous components and support a wide range of applications. Mapping aging rate measures to fit within the boundaries of these tools is non-trivial. In this paper, a framework for modeling and controlling the aging rate of batteries based on Markov process theory is presented. Numerical results illustrate the tradeoff between battery degradation and task completion delay enabled by the proposed framework
4th International Conference on Laser, Optics, and Optoelectronic Technology (LOPET 2024)
No full textFront matter of the Proceedings of SPIE, 277-786X, Volume 13231, 4th International Conference on Laser, Optics, and Optoelectronic Technology (LOPET 2024), 17-19 May 2024, Chongqing, China. Suihu Dang, Manuel Filipe Costa (eds), SPIE, August 2024. https://doi.org/10.1117/12.304452
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